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Polylactic-co-glycolic acid (PLGA)/calcium carbonate compound microsphere with porous shell and preparation method for compound microsphere

A technology of composite microspheres and calcium carbonate, which is applied in the field of preparation of degradable composite microspheres, can solve problems such as patient trauma, achieve the effects of avoiding residues, facilitating growth, and improving preparation methods

Active Publication Date: 2012-11-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preformed stents have good mechanical properties and structural diversity, but they need to be implanted by surgery, which often causes greater trauma to patients

Method used

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  • Polylactic-co-glycolic acid (PLGA)/calcium carbonate compound microsphere with porous shell and preparation method for compound microsphere
  • Polylactic-co-glycolic acid (PLGA)/calcium carbonate compound microsphere with porous shell and preparation method for compound microsphere
  • Polylactic-co-glycolic acid (PLGA)/calcium carbonate compound microsphere with porous shell and preparation method for compound microsphere

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 1g of PLGA (50 / 50, Mw=30kDa) and dissolve it in 20ml of dichloromethane to obtain 20ml of PLGA organic solution. Weigh 0.5 g of calcium carbonate powder and add it into the PLGA organic solution, and stir for 10 min at 300 rpm and 300 w ultrasonic power to obtain a PLGA / calcium carbonate mixed solution. Weigh 5g of PVA and add it to 500ml of deionized water, stir for 15min, then heat to 90°C to dissolve the PVA, and obtain a PVA aqueous solution after cooling. Weigh 0.5 g of gluconolactone and dissolve in 500 ml of PVA aqueous solution to obtain an acidic PVA aqueous solution. The PLGA / calcium carbonate mixed solution was added dropwise into the acidic PVA aqueous solution under the stirring condition of 350rpm to obtain an oil-in-water single emulsion. Stir the emulsion continuously (350rpm) in a fume hood for 20 hours to volatilize the dichloromethane in the emulsion and solidify the oil droplets into balls. The obtained microspheres were collected, washed 3 t...

Embodiment 2

[0031] Weigh 2g of PLGA (50 / 50, Mw=30kDa) and dissolve it in 10ml of dichloromethane to obtain 10ml of PLGA organic solution. Weigh 0.2 g of calcium carbonate powder and add it into the PLGA organic solution, and stir for 10 min at 300 rpm and 300 w ultrasonic power to obtain a PLGA / calcium carbonate mixed solution. Weigh 2g of PVA and add it to 250ml of deionized water, stir for 15min, then heat to 90°C to dissolve the PVA, and obtain a PVA aqueous solution after cooling. Weigh 0.3 g of gluconolactone and dissolve in PVA aqueous solution to obtain acidic PVA aqueous solution. The PLGA / calcium carbonate mixed solution was added dropwise into the acidic PVA aqueous solution under the stirring condition of 400rpm to obtain an oil-in-water single emulsion. Keep stirring (400rpm) the emulsion in a fume hood for 20 hours to volatilize the dichloromethane in the emulsion and solidify the oil droplets into balls. The obtained microspheres were collected, washed 3 times with deioniz...

Embodiment 3

[0033] Weigh 1g of PLGA (50 / 50, Mw=30kDa) and dissolve it in 10ml of dichloromethane to obtain 10ml of PLGA organic solution. Weigh 0.05g of calcium carbonate powder and add it to the PLGA organic solution, and stir for 10min at a maximum speed of 300rpm and 300w ultrasonic power to obtain a mixed solution of PLGA / calcium carbonate. Weigh 2g of PVA and add it to 250ml of deionized water, stir for 15min, then heat to 90°C to dissolve the PVA, and obtain a PVA aqueous solution after cooling. Weigh 0.4g of gluconolactone and dissolve in PVA aqueous solution to obtain acidic PVA aqueous solution. The PLGA / calcium carbonate mixed solution was added dropwise into the acidic PVA aqueous solution under the stirring condition of 400rpm to obtain an oil-in-water single emulsion. Keep stirring (250rpm) the emulsion in a fume hood for 20 hours to volatilize the dichloromethane in the emulsion and solidify the oil droplets into balls. The obtained microspheres were collected, washed 3 ti...

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Abstract

The invention discloses a polylactic-co-glycolic acid (PLGA) / calcium carbonate compound microsphere with a porous shell and a preparation method for the compound microsphere. The preparation method comprises the following steps of: a, dissolving PLGA in a methylene dichloride organic solvent to obtain a PLGA oil phase; adding calcium carbonate powder to the PLGA oil phase; stirring and performing ultrasonic treatment to obtain organic / inorganic uniform mixed liquid; b, dissolving glucolactone in polyvinyl alcohol (PVA) aqueous solution to obtain acid PVA aqueous solution; c, dispersing the mixed liquid obtained in the step a into the acid PVA aqueous solution obtained in the step b under the condition of stirring to obtain oil-in-water single emulsion; d, continue stirring under the condition of reduced pressure to enable methylene dichloride in an oil phase drop to volatilize to obtain solidified compound microspheres; and e, collecting the microspheres in the step d; washing by using deionized water; and performing freeze drying. A pore-forming agent is not introduced by the method disclosed by the invention, thus, a porous structure exists on the surface of the prepared compound microsphere, and the compound microsphere is in favor of cell growth.

Description

technical field [0001] The invention relates to a preparation method of degradable composite microspheres, in particular to a preparation method of PLGA / calcium carbonate composite microspheres with a porous shell. Background technique [0002] Polylactic-glycolic acid (PLGA) is an FDA-approved biomaterial with good biocompatibility and degradability. PLGA microspheres are good carriers of various drugs and genes, and can effectively realize the sustained release of drugs. However, the degradation products of PLGA itself are acidic, which may inactivate the loaded drugs and cause immune rejection in the body. In order to overcome this problem, people often compound calcium phosphate substances such as HA and β-TCP in the PLGA matrix. Adding calcium phosphate substances can not only neutralize the acidic degradation products of PLGA, but also improve the mechanical properties and osteoconductivity of PLGA matrix. However, calcium phosphate substances tend to degrade slowly...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/18A61L27/02A61L27/50A61L27/56B01J13/04C08J9/26C08J3/12C08L67/04C08K3/26
CPCB01J13/125B01J13/04C08J2367/04A61L27/46A61L27/56A61L27/18C08J3/212C08J3/12A61L27/50A61L27/02C08J9/26
Inventor 王迎军程德林曹晓东
Owner SOUTH CHINA UNIV OF TECH
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