Method for processing papermaking wastewater and producing microbial oil, and its special bacterial strain

A technology of papermaking wastewater and microbial strains, applied in the method and its special strains, in the field of treating papermaking wastewater and producing microbial oil, can solve the problems that raw materials cannot meet the needs, the quality of different sources is difficult to control, and there are potential safety hazards, etc., so as to reduce pollution Emission problems, realization of resource utilization, and high practical value

Inactive Publication Date: 2012-11-21
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES +1
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  • Application Information

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Problems solved by technology

However, the cost of raw materials is high, and there is a problem of "competition with farmers for grain (oil) and land with grain (oil)"
Woody oil crops have stable quality and low cost, but they are mainly tropical plants. Due to geographical restrictions, the raw materials are difficult to meet

Method used

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  • Method for processing papermaking wastewater and producing microbial oil, and its special bacterial strain
  • Method for processing papermaking wastewater and producing microbial oil, and its special bacterial strain
  • Method for processing papermaking wastewater and producing microbial oil, and its special bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, preparation and identification of Rhodococcus sp. LDS5

[0045] Rhodococcus sp. (Rhodococcus sp.) RHA1 (on December 8, 2010 was preserved in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures, and its address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the People's Republic of China, The Institute of Microbiology, Chinese Academy of Sciences, classified as Rhodococcus sp., and the registration number of the collection center: CGMCC No. 4423) knocked out the LDS5 gene to obtain Rhodococcus sp. (Rhodococcus sp.) LDS5. Rhodococcus sp. LDS5 was deposited on April 12, 2011 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the registration number of the collection center is: CGMCC No.4762.

[0046] Rhodococcus sp. LDS5CGMCC No.4762 rod-shaped bacteria ( figure 1 ), do not form conidia or endospores. The colonies are pale yellow opaque,...

Embodiment 2

[0047] Example 2. A method for producing triglycerides by cultivating Rhodococcus sp. LDS5CGMCC No.4762 in papermaking wastewater.

[0048] 1. Cultivate triglyceride detection in different wastewater

[0049] In order to find low-cost culture conditions, which are beneficial to large-scale culture. Low nitrogen and high carbon have become the main conditions for finding low-cost cultivation. Pulping and papermaking wastewater contains a large amount of organic matter, and it is rich in carbon sources and low in nitrogen. Using wastewater to cultivate strains, if they can grow, can not only obtain the supply of biodiesel raw materials, but also may promote the development of wastewater treatment industry. Therefore, this experiment selects the seriously polluted pulping wastewater CTMP wastewater and APMP wastewater in the paper industry, and checks the oil production of bacteria. The experimental steps are as follows:

[0050] Bacterial culture: Cultivate Rhodococcus sp. LD...

Embodiment 3

[0057] Embodiment 3, use Rhodococcus (Rhodococcus sp.) LDS5CGMCC No.4762 to treat papermaking wastewater

[0058] Bacteria with concentration gradient and time gradient are cultivated, and some wastewater indicators are detected for these bacteria. The cultivation method is as follows:

[0059] Concentration gradient: single clones of Rhodococcus sp. LDS5CGMCC No.4762 and Rhodococcus sp. RHA1CGMCC No.4423 were respectively inserted into 300ml NB medium and cultured at 30°C for 36h. Take 50ml, 20ml, and 10ml of 5000g culture solution, centrifuge at 4°C for 10 minutes, discard the supernatant, and transfer the centrifuged bacteria to 100ml of CTMP waste water (COD Cr 10053mg / L, BOD 5 9210mg / L), that is, the transfer concentrations were 1:2, 1:5, and 1:10, respectively. At the same time, 100ml of the same batch of CTMP wastewater was taken as a control. 30°C, 200 rpm shaking culture for 48 hours, centrifuge the liquid at 4000g, 4°C for 10 minutes, take the supernatant to dete...

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Abstract

The invention discloses a method for processing papermaking wastewater and producing biological oil, and its special bacterial strain Rhodococcus sp. LDS5. According to the method, the Rhodococcus sp. utilizes the papermaking wastewater as a culture medium to produce the biological oil, and simultaneously organic matters in the papermaking wastewater are degraded. The method comprises the following steps: inoculating the Rhodococcus sp. LDS5 or a bacterial agent treating the Rhodococcus sp. LDS5 as an active component into the papermaking wastewater, and culturing to obtain Rhodococcus sp. LDS5 thalli; and extracting from thalli to obtain triglyceride. According to the method, the COD removal rate and the BOD removal rate of the papermaking wastewater reach above 55% and above 75% respectively. The method for producing the microbial oil has the advantages of simple operation, low cost and high practical value, can effectively solve a papermaking wastewater pollution discharge problem, enables conversion of wastes into biological oil as biodiesel raw material, and realizes the resource utilization of the papermaking waste.

Description

technical field [0001] The invention relates to a method for treating papermaking wastewater, in particular to a method for treating papermaking wastewater and producing microbial oil and special strains thereof. Background technique [0002] The pulp and paper industry is an important basic raw material industry in my country's national economy, but it is also a major producer of "three wastes" pollution, especially water pollution. billion tons, accounting for 18.76% of the total national industrial wastewater discharge of 21.738 billion tons. The chemical oxygen demand (COD) in the discharged wastewater is 1.288 million tons, accounting for 31.82% of the total national industrial COD discharge of 4.048 million tons. [0003] At present, the methods of pulp and paper industry wastewater treatment mainly include physical method, biochemical method, chemical method and the combination of the three methods. The removal of insoluble pollutants; the biochemical method mainly u...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/64C02F3/34C12R1/01C02F103/28
CPCY02E50/13Y02E50/10
Inventor 刘平生杜雅兰丁云峰张淑妍冯文英苏振华徐明曹春昱
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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