Heart-type fatty acid binding protein detection reagent kit and preparation method thereof

A fatty acid binding and protein detection technology, applied in the field of biomedicine, can solve the problems of being unsuitable for routine testing, wasting resources, and taking a long time

Inactive Publication Date: 2012-11-21
乐普(北京)诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods have the disadvantages of cumbersome operation, long time cons...

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The kit of the present invention is exemplified as a double reagent, wherein:

[0034] Reagent R1:

[0035] Tris buffer 200mmol / L

[0036] Polyethylene glycol 8 mmol / L

[0037] Sodium azide 5 mmol / L

[0038] Sodium edetate 5mmol / L

[0039] Bovine serum albumin 5mmol / L;

[0040] Reagent R2:

[0041] Tris buffer 200mmol / L

[0042] Latex particles conjugated with heart fatty acid binding protein monoclonal antibody 5% (v / v)

[0043] Tween-20 5mmol / L

[0044] Sodium azide 3 mmol / L

[0045] Sodium edetate 5mmol / L

[0046] Bovine serum albumin 5mmol / L.

Embodiment 2

[0047] Embodiment 2: Kit usage method.

[0048] 1. Reagent preparation, the reagent is a liquid double reagent, ready to use after opening the bottle, of which:

[0049] Reagent R1:

[0050] Tris buffer 200mmol / L

[0051] Polyethylene glycol 8 mmol / L

[0052] Sodium azide 5 mmol / L

[0053] Sodium edetate 5mmol / L

[0054] Bovine serum albumin 5mmol / L;

[0055] Reagent R2:

[0056] Tris buffer 200mmol / L

[0057] Latex particles conjugated with heart fatty acid binding protein monoclonal antibody 5% (v / v)

[0058] Tween-20 5mmol / L

[0059] Sodium azide 3 mmol / L

[0060] Sodium edetate 5mmol / L

[0061] Bovine serum albumin 5mmol / L.

[0062] 2. Automatic biochemical analyzer parameter setting

[0063] (a) Detection temperature: 37°C

[0064] (b) Detection wavelength: main wavelength is 500nm; secondary wavelength is 700nm;

[0065] (c) Reaction time: 8 minutes and 20 seconds, wherein the incubation time is 3 minutes, the reaction time is 100 seco...

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Abstract

A heart-type fatty acid binding protein detection reagent kit, the kit body of the reagent kit comprises two reagents inside: reagent R1: buffer solution, polyethylene glycol, sodium azide, sodium ethylene diamine tetracetate, and bovine serum albumin; reagent R2: buffer solution, latex particles bound with heart-type fatty acid binding protein monoclonal antibody, Tween-20, sodium azide, sodium ethylene diamine tetracetate, and bovine serum albumin. Mixing a sample with the reagents in a specific volumetric ratio to conduct a series of reactions, placing the reactant under a semi/full automatic biochemical analyzer, and detecting the change speed of light absorbance at the position of 500nm dominant wavelength, thus the concentration of heart-type fatty acid binding protein can be figured out. The reagent kit has the advantages of accuracy, stability and convenience.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a detection kit for detecting human-shaped fatty acid binding protein. Background technique [0002] Heart-type fatty acid binding protein (H-FABP) is a soluble cytoplasmic protein with a molecular weight of 14-15kd, which is mainly distributed in myocardial tissue, accounting for about 4%-8% of the soluble protein in cardiomyocytes, in skeletal muscle, renal tubules, brain There is also a small amount of distribution in tissues, mammary glands, and placenta tissues. H-FABP is an acidic protein composed of 132 amino acid residues, its isoelectric point (PI) is 5, and its gene is located on chromosome 1. [0003] Under normal conditions, long-chain fatty acids are the main source of energy for cardiomyocytes. As a carrier protein of fatty acids, H-FABP combines with the long-chain fatty acids in the myocardium, transports them from the plasma membrane to the mito...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 谢兵
Owner 乐普(北京)诊断技术股份有限公司
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