Method for separating and purifying high-purity sisomicin

Abstract

The invention discloses a method for separating and purifying high-purity sisomicin, which belongs to the technical field of membrane separation combined continuous chromatographic separation and purification of organic compounds. The high-purity sisomicin is separated from a sisomicin strippant by using a continuous chromatographic separation technology and purifying. A system combining ultra and nano-filtration membrane technologies with continuous chromatographic separation is adopted, so that a reasonable temperature condition is controlled in a membrane separation production process, the generation of degradation products is reduced, the production cost can be reduced simultaneously, the production period is shortened, the product quality is enhanced, and the discharge of waste water is reduced; and a sisomicin feed liquid can be separated and purified more effectively through a continuous chromatographic separation system. According to measurement with high performance liquid chromatography (HPLC), the sisomicin purity of a separated and purified product is more than or equal to 95 percent.

Description

technical field [0001] The present invention relates to a separation and purification method of high-purity sisomycin, which mainly uses ultra- and nanofiltration membrane technology to reduce the generation of degraded substances of sisomycin by controlling the temperature conditions in the membrane separation process; The chromatographic separation technique is a method for separating and purifying the by-product produced during the extraction process of sisomycin to obtain high-purity sisomycin. The invention belongs to the technical field of membrane separation combined with continuous chromatographic separation and purification of organic compounds. Background technique [0002] Sithomycin is an aminoglycoside antibiotic that has strong antibacterial activity against Gram-positive and Gram-negative bacteria. The clinical efficacy is similar to that of kanamycin, but the toxicity to the kidney and the eighth cranial nerve is significantly less than that of kanamycin. It...

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Problems solved by technology

This production process has the disadvantages of low production capacity, low output, long production cycle, huge fixed investment, and large floor area.

[0006] 2) Due to the large amount of resin used, the amount of acid and alkali required to activate the resin is also large (including water). The unit acid consumption per kg of sisomycin is 25 kg of concentrated hydrochloric acid, 10 kg of liquid alkali, and 5 kg of liquid ammonia, and the amount of wastewater discharged is large. , especially waste water containing ammonia, the environmental protection pressure is high

[0007] 3) The properties of sisomycin and the main impurity are very similar, and the separation effect is poor. In the resin desorption and absorption collection liquid, the mixture of sisomycin and impurities accounts for a large proportion; the purity of sisomycin collection liquid is low, and the first pass rate is low , needs repeated column separation, the yield is only 45%-60%

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