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Method for preparing immobilized glucose oxidase by taking PDDA-Fe3O4 nano particles as carriers

A technology of glucose oxidase and nanoparticles, which is applied in the direction of immobilizing on or in inorganic carriers, or in or on organic carriers, which can solve the problems of hindering the industrial application and promotion of enzyme preparations and the poor stability of glucose oxidase , Enzyme preparations can no longer be used and other problems, to achieve the effect of easy recycling, simple technology, high thermal stability and pH stability of the enzyme

Inactive Publication Date: 2012-11-28
SHAOXING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the free glucose oxidase has disadvantages such as poor stability in use and inability to recover after enzyme treatment, which leads to the inability of the enzyme preparation to be reused, which is not conducive to the actual development and application. These problems hinder the industrial application and promotion of the enzyme preparation

Method used

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  • Method for preparing immobilized glucose oxidase by taking PDDA-Fe3O4 nano particles as carriers
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  • Method for preparing immobilized glucose oxidase by taking PDDA-Fe3O4 nano particles as carriers

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Embodiment 1

[0021] A kind of PDDA-Fe in this embodiment 3 o 4 A method for preparing immobilized glucose oxidase with nanoparticles as a carrier, comprising the following steps: 1) using polydiallyldimethylamine hydrochloride (PDDA) solution, FeCl 3 ·6H 2 O solution and FeCl 2 4H 2 O as raw material to prepare PDDA-Fe 3 o 4 Magnetic nanoparticles; as described in step 1) using diallyldimethylamine hydrochloride (PDDA) solution, FeCl 3 ·6H 2 O solution and FeCl 2 4H 2 O is the raw material in a three-necked flask, in which the mass concentration of diallyldimethylamine hydrochloride (PDDA) solution is 4%; FeCl 3 ·6H 2 The molar concentration of O solution is 0.2mol / L; FeCl 2 4H 2 The molar concentration of O is 0.1mol / L; and the diallyldimethylamine hydrochloride (PDDA) solution, FeCl 3 ·6H 2 O solution and FeCl 2 4H 2 The volume ratio of O is 1:4:4. Then, the NaOH solution was slowly added dropwise under the stirring of a precision booster electric stirrer, the reaction t...

Embodiment 2

[0026] A kind of PDDA-Fe in this embodiment 3 o 4 A method for preparing immobilized glucose oxidase with nanoparticles as a carrier, comprising the following steps: 1) using polydiallyldimethylamine hydrochloride (PDDA) solution, FeCl 3 ·6H 2 O solution and FeCl 2 4H 2 O as raw material to prepare PDDA-Fe 3 o 4 Magnetic nanoparticles; as described in step 1) using diallyldimethylamine hydrochloride (PDDA) solution, FeCl 3 ·6H 2 O solution and FeCl 2 4H 2 O is the raw material in a three-necked flask, in which the mass concentration of diallyldimethylamine hydrochloride (PDDA) solution is 4%; FeCl 3 ·6H 2 The molar concentration of O solution is 0.2mol / L; FeCl 2 4H 2 The molar concentration of O is 0.1mol / L; and the diallyldimethylamine hydrochloride (PDDA) solution, FeCl 3 ·6H 2 O solution and FeCl 2 4H 2 The volume ratio of O is 1:4:4. Then, the NaOH solution was slowly added dropwise under the stirring of a precision booster electric stirrer, the reaction t...

Embodiment 3

[0031] A kind of PDDA-Fe in this embodiment 3 o 4 A method for preparing immobilized glucose oxidase with nanoparticles as a carrier, comprising the following steps: 1) using polydiallyldimethylamine hydrochloride (PDDA) solution, FeCl 3 ·6H 2 O solution and FeCl 2 4H 2 O as raw material to prepare PDDA-Fe 3 o 4 Magnetic nanoparticles; as described in step 1) using diallyldimethylamine hydrochloride (PDDA) solution, FeCl 3 ·6H 2 O solution and FeCl 2 4H 2 O is the raw material in a three-necked flask, in which the mass concentration of diallyldimethylamine hydrochloride (PDDA) solution is 4%; FeCl 3 ·6H 2 The molar concentration of O solution is 0.2mol / L; FeCl 2 4H 2 The molar concentration of O is 0.1mol / L; and the diallyldimethylamine hydrochloride (PDDA) solution, FeCl 3 ·6H 2 O solution and FeCl 2 4H 2 The volume ratio of O is 1:4:4. Then, under the stirring of a precision booster electric stirrer, NaOH solution was slowly added dropwise, the reaction temp...

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Abstract

The invention discloses a method for preparing immobilized glucose oxidase by taking PDDA-Fe3O4 nano particles as carriers. The method comprises the following steps of: 1) preparing PDDA-Fe3O4 magnetic nano particles by taking a poly(diallyldimethylammonium chloride) solution, a FeCl3.6H2O solution and FeCl2.4H2O as raw materials; and 2) preparing the immobilized glucose oxidase by taking the PDDA-Fe3O4 magnetic nano particles as the carriers. The method has the characteristics that a technology is simple and practicable, the prepared carriers are high in specific surface area, biocompatibility, enzyme load capacity, enzymatic activity and heat stability, and pH stability, and the immobilized enzyme is easy to recycle under the action of an external magnetic field.

Description

technical field [0001] The present invention relates to a kind of PDDA-Fe 3 o 4 Magnetic nanoparticles immobilized glucose oxidase and its preparation method. Background technique [0002] With the development of biochemical engineering technology and the improvement of textile green processing requirements, textile bio-enzyme pretreatment has been recognized as a printing and dyeing processing method that meets environmental protection requirements. Among them, glucose oxidase can catalyze glucose to generate hydrogen peroxide for textile bleaching. At the same time, the generated gluconolactone can be further hydrolyzed to generate gluconic acid, which can chelate metal ions that can catalyze the decomposition of hydrogen peroxide in water, so that no bleaching process occurs. Additional stabilizers need to be added, which reduces the amount of chemical reagents and environmental pollution, and is a promising biological bleaching technology. [0003] However, free gluco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/08
Inventor 白刚刘艳春钱红飞
Owner SHAOXING UNIVERSITY