Method for detecting hypochlorite

A technology for detecting hypochlorous acid and solution, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of difficult synthesis, complex reagent structure, etc., and achieve cost The effect of low cost, high sensitivity and selectivity, and simple detection means
CN102818802AInactive Publication Date: 2012-12-12SHANXI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANXI UNIV
Publication Date
2012-12-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for detecting hypochlorite. The method can quantitatively detect hypochlorite based on 2-pyridylaldehyde fluorescein hydrazone (FHP) and Cu<+>. The method is concretely characterized in that the Cu<+> is oxidized into Cu<2+> by the hypochlorite in an HEPES buffer solution with the pH of 7, and a complex is prepared from the Cu<2+> and the FHP, so a change in an ultraviolet-visible spectrum appears, thereby the detection of the hypochlorite is realized. The detection method has the advantages of high sensitivity and selectivity to the hypochlorite, simple, sensitive and rapid detection process, and accurate detection result.
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Description

technical field

[0001] The invention relates to a hypochlorite detection technology, in particular to a method for rapidly and quantitatively detecting hypochlorite. Background technique

[0002] NaOCl is widely used as a bleach and disinfectant in a concentration range of 10 -5 ~10 -2 mol / L. Its protonated form (hypochlorous acid, HClO), is a biologically important reactive oxygen species (ROS), used as an antimicrobial in organisms and in water treatment. However, excess HClO can cause tissue damage and diseases such as: hepatic ischemia-reperfusion injury, atherosclerosis, lung injury, rheumatoid and cardiovascular diseases, neuronal degeneration, arthritis and cancer.

[0003] Currently reported methods for detecting hypochlorite include: colorimetric method (S.M.Chen, J.X.Lu, C.D.Sun, H.M.Ma.Analyst 135(2010) 577–582), luminescence method (J.Ballesta-Claver, M.C.Valencia- Miron, L.F.Capitán-Vallvey.Anal.Chim.Acta.522(2004)267–273), electrochemical method (A.P.Soldat...

Claims

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