Method for solid liquid mixed culture of medical hericium erinaceus mycelia
A liquid mixing and solid medium technology, applied in the field of microbial cultivation, can solve the problems of short cycle, high efficiency, difficult monitoring and control of fermentation parameters, and difficult stirring, etc., and achieve the effect of high immune activity
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Embodiment 1
[0026] A method for solid-liquid mixed culture of medicinal Hericium erinaceus mycelium, comprising the following steps: the percentages of the following substances are all percentages by weight,
[0027] (1). Preparation of solid medium
[0028]
[0029] Add the rest of the water, the pH is natural, mix evenly, and sterilize at 121°C for 30 minutes;
[0030] (2).Optimized screening of strains and isolation of fruiting bodies:
[0031] Pick the fruiting bodies of wild Hericium erinaceus in the primeval forest of Zhongtiao Mountain, which are strong, well-developed, free from diseases and insect pests, and whose seeds are not scattered;
[0032] In the ultra-clean workbench above grade 100 in the inoculation room, immerse the fruiting body of Hericium erinaceus in 75% alcohol for 2 minutes, take it out, rinse it with distilled water, dry it with sterile gauze, and put the fruiting body on a petri dish , cover it with a bell jar or similar utensils, keep the room temperatur...
Embodiment 2
[0045] A method for solid-liquid mixed culture of medicinal Hericium erinaceus mycelium, comprising the following steps: the percentages of the following substances are all percentages by weight,
[0046] (1). Preparation of solid medium
[0047]
[0048] Add the remaining amount of water, the pH value is 7.5, mix well, and sterilize at 118°C for 30 minutes;
[0049] (2).Optimized screening of strains and isolation of fruiting bodies:
[0050] Pick the fruiting bodies of wild Hericium erinaceus in the primeval forest of Zhongtiao Mountain, which are strong, well-developed, free from diseases and insect pests, and whose seeds are not scattered;
[0051] In the ultra-clean workbench above grade 100 in the inoculation room, immerse the fruiting body of Hericium erinaceus in 75% alcohol to disinfect for 1 minute, take it out, rinse it with distilled water, dry it with sterile gauze, and put the fruiting body on a petri dish , cover it with a bell jar or similar utensils, keep t...
Embodiment 3
[0064] A method for solid-liquid mixed culture of medicinal Hericium erinaceus mycelium, comprising the following steps: the percentages of the following substances are all percentages by weight,
[0065] (1). Preparation of solid medium
[0066]
[0067] Add the remaining amount of water, the pH value is 6, mix well, and sterilize at 121°C for 25 minutes;
[0068] (2).Optimized screening of strains and isolation of fruiting bodies:
[0069] Pick the fruiting bodies of wild Hericium erinaceus in the primeval forest of Zhongtiao Mountain, which are strong, well-developed, free from diseases and insect pests, and whose seeds are not scattered;
[0070] In the ultra-clean workbench above grade 100 in the inoculation room, immerse the fruiting body of Hericium erinaceus in 75% alcohol for 2 minutes, take it out, rinse it with distilled water, dry it with sterile gauze, and put the fruiting body on a petri dish , cover it with a bell jar or similar utensils, keep the room temp...
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