Solid fermentation method of straw fermentation bio-additive and application

A technology for fermenting organisms and solid fermentation, applied in the field of environmental microorganisms and microbial fermentation, can solve the problems of high production cost, poor production stability, affecting the industrialization process, etc., to improve quality and effectiveness, reduce pH value, and simple fermentation method. easy effect

Inactive Publication Date: 2012-12-26
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in practical application, the bacterial agent has the disadvantages of consuming a lot of manpower and material resources in mass production, poor production stability, and high production cost, which

Method used

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  • Solid fermentation method of straw fermentation bio-additive and application
  • Solid fermentation method of straw fermentation bio-additive and application
  • Solid fermentation method of straw fermentation bio-additive and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of inoculum liquid:

[0021] (1) Take 1ml of each of Streptococcus faecalis, Pediococcus cerevisiae, Lactobacillus plantarum, Lactobacillus casei, Lactobacillus daimeri and Bacillus licheniformis respectively inoculated in 100ml culture medium that has been sterilized by high temperature (20min, 121℃) cultured at 30°C for 48 hours. The formula of the culture solution is 4-6g of yeast extract, 8-15g of glucose, 5-10g of peptone, 1-3g of potassium dihydrogen phosphate, 1000ml of water, and pH 6.0-7.0.

[0022] (2) Take a total of 600ml of six individually cultured bacterial solutions, add them to 11.4 L of the same high-temperature sterilized (20min, 121°C) culture solution (same formula as above) for mixed culture, and incubate at 30°C for 48h. Checked with a microscope, the number of bacteria in the culture solution reached 10 8 1 / ml is qualified, and 12L of mixed bacterial solution is obtained, which is the required liquid bacterial species.

Embodiment 2

[0024] Comparison of bacterial counts between liquid fermentation and solid fermentation of "Straw Fermentation Biological Additives"

[0025] (1) Liquid fermentation

[0026] Prepare 3L of culture medium (same formula as above), put it into a 5L fermenter, sterilize at 121°C for 20 minutes, add 300ml of mixed culture liquid strains, and put it at a temperature of 30°C, pH 5.0, stirring speed 300 r / min, ventilation volume 4 Under the optimal fermentation condition of L / min, the mixed bacterial liquid reached the end of fermentation in 24 hours, and the maximum concentration of bacterial liquid at the end of fermentation could reach 27.5×10 8 more than one / ml.

[0027] (2) Solid fermentation

[0028] Mix water: soybean meal powder: wheat bran powder: sucrose: urea at a ratio of 400:200:200:10:5, mix evenly and distribute, each bottle is 200-400g, sterilized at 121°C for 15 minutes, and each bottle is filled with 20mL of liquid Bacteria were cultured statically at 35°C for 48...

Embodiment 3

[0038] (1) Mix the solid medium and put it into jars, and sterilize it at 121°C for 15 minutes. The weight-to-number ratio of the solid medium is water: soybean meal powder: wheat bran powder: sucrose: urea Respectively 400:200:200:10:5;

[0039](2) Inoculate the liquid strains of the mixed culture according to 20% of the inoculum, and ferment and culture them in a constant temperature incubator at 35°C for 72 hours;

[0040] (3) After fermentation and natural drying at 30°C for 5 days, the number of bacteria reached 700×10 8 pcs / g; the liquid strain mentioned therein refers to: straw fermentation biological additive.

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Abstract

The invention discloses a solid fermentation method of a straw fermentation bio-additive. The method is characterized by comprising the following steps of: mixing solid media according to the ratio of water to bean pulp powder to wheat bran powder to cane sugar to urea being 400:200:200:10:5; split charging into wild-mouth bottles after uniformly mixing; sterilizing for 20 minutes at the temperature of 121 DEG C; inoculating a straw fermentation bio-additive liquid strain into each bottle according to the proportion of 10-20 percent; and carrying out static cultivation for 48-72h at the temperature of 35 DEG C, wherein after natural airdrying, the microbial content in fungicide reaches 700*108/g. According to the method, the production cost can be reduced, the product quality and the effectiveness are improved; and when the method is applied to water body purification, the nitrite in a water body can be degraded, a pH value of the water body is reduced, the water body is purified, and wide application prospect is realized.

Description

technical field [0001] The invention relates to the technical field research of environmental microorganisms and microbial fermentation technology, and more specifically relates to a solid fermentation method of straw fermentation biological additives and its application in water body purification. Background technique [0002] This patent is based on the patent "Straw Fermentation Biological Additive and Its Configuration Method" (Patent No. ZL97119176.X) to improve the production technology and application range. "Straw Fermentation Biological Additive" consists of 6 L-type lactic acid homogeneous lactic acid bacteria of Streptococcus faecalis, Pediococcus cerevisiae, Lactobacillus plantarum, Lactobacillus casei, Lactobacillus daimeri and Bacillus licheniformis according to the ratio of 1:1:1:1: The ratio of 1:1 is scientifically compatible, and it is fermented through bioengineering technology. It can make wheat straw, rice straw and yellow dry corn straw into a kind of ...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F1/66C02F3/34C12R1/46C12R1/01C12R1/25C12R1/225C12R1/10
Inventor 张峰峰谢凤行周可赵玉洁
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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