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Quality control method of Sijunzi (Chinese name) decoction

A quality control method, the technology of Sijunzi Decoction, is applied in the field of quality control of Sijunzi Decoction to achieve good reproducibility, simple quality standards and controllable quality

Inactive Publication Date: 2012-12-26
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Aiming at the deficiencies in the prior art, the present invention adopts new means to control the quality of Sijunzi Decoction, especially adopts dual-wavelength high-performance liquid chromatography to simultaneously measure ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and glycyrrhizic acid in the prescription. The content of the four index components solves the problem of quality control of Sijunzi Decoction, and the present invention provides a quality control method for Sijunzi Decoction

Method used

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  • Quality control method of Sijunzi (Chinese name) decoction

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Embodiment 1

[0019] The content determination method for simultaneous determination of four components is:

[0020] Medicinal materials, instruments and reagents:

[0021] Ginseng (Panax ginseng C.A.Mey.; produced in Jilin, batch number 110710), Atractylodes macrocephala (Atractylodes macrocephala Koidz.; produced in Zhejiang, batch number 110306), Poria cocos (Poria cocos (Schw.) Wolf; produced in Anhui, batch number 110513) in Sijunzi Decoction , Glycyrrhizae Radix et Rhizoma Praeparata cum Melle (Glycyrrhizae Radix et Rhizoma Praeparata cum Melle; produced in Inner Mongolia, batch number 110519) were purchased from Nanjing Haiyuan Chinese Herbal Pieces Co., Ltd.

[0022] Waters2695 high-performance liquid chromatography; Waters2589 detector; UNIQUE-S15 ultrapure water generator; BP211D electronic balance (Germany Sartorius company); KQ-500DE medical numerical control ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0023] Ginsenoside Rg1 reference substance (National Ins...

Embodiment 2

[0073] The multi-index simultaneous determination method of four components in Sijunzi Decoction is as follows:

[0074] Chromatographic conditions Chromatographic column: octadecyl bonded silica gel as filler; mobile phase: acetonitrile-0.03% formic acid solution; flow rate: 0.9mL / min; column temperature: 30°C; injection volume: 20μL. Dual wavelength detection: 202nm, 250nm. Gradient elution program: 0~14min, 19% acetonitrile; 14~15min, 20.7% acetonitrile; 15~28min, 20.7% acetonitrile; 28~33min, 29% acetonitrile; 33~47min, 41% acetonitrile; 47~52min, 41 % acetonitrile.

[0075] Preparation of Reference Substance Solution Accurately weigh appropriate amounts of ginsenoside Re, ginsenoside Rg1, ginsenoside Rb1, and ammonium glycyrrhizinate reference substance, add methanol to dissolve, and make ginsenoside Rb1, ginsenoside Re, and ginsenoside Rg1 with concentrations of 0.7995 mg respectively / mL, 0.3420mg / mL, 0.3805mg / mL mixed standard solution and ammonium glycyrrhizinate re...

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Abstract

The invention discloses a quality control method of Sijunzi (Chinese name) decoction. The content of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and glycyrrhizic acid is measured at the same time by the high performance liquid chromatography; the content measurement method of the four ingredients comprises the following steps of: preparation of a reference solution, preparation of a test solution and content measurement, wherein the ginsenoside Re, the ginsenoside Rg1, the ginsenoside Rb1, and glycyrrhizic acid in the ammonium glycyrrhetate content in set values respectively are 12.0mg, 10.0mg, 18.0mg and 28.0mg. The quality standard is simple, and the quality of a drug can be better controlled. The quality control method disclosed by the invention is strong in specificity and good in reproducibility, so that the security, effectiveness and quality control of the drug are really reflected.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicine preparations, in particular to a quality control method for Sijunzi Decoction. Background technique [0002] Sijunzi Decoction is derived from "Taiping Huimin Mixture Bureau Prescription", which is used to treat spleen and stomach qi deficiency. It is the basic prescription for treating spleen and stomach qi deficiency. In the prescription, ginseng is the king, which is sweet, warm and invigorating, invigorating the spleen and nourishing the stomach. Atractylodes macrocephala is bitter and warm, invigorates the spleen and dries dampness, strengthens the power of invigorating qi and assisting transportation, and is a ministerial drug. Poria cocos is sweet and light, invigorating the spleen and expelling dampness, and it is an adjuvant drug; cocoons and rhizomes are used together to invigorate the spleen and eliminate dampness. Roasted licorice is sweet and warm, nour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 吴皓郁红礼徐秋香陆兔林宫乐
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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