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Porcine complex probiotics preparations and preparation method thereof

A technology of compound probiotics and preparations, applied in the fields of application, animal feed, animal feed, etc., can solve the problems of drug residues, animal product quality decline, animal disease resistance reduction, etc.

Inactive Publication Date: 2013-01-09
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Since the 20th century, antibiotics and antimicrobials have been widely used in livestock production, but drug residues, drug resistance, reduced animal disease resistance and reduced quality of animal products caused by long-term use have become a serious and urgent problem to be solved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation of the liquid dosage form of compound probiotic preparation for pigs, the steps are as follows:

[0051] (1) Preparation of Lactobacillus rhamnosus preparations: take Lactobacillus rhamnosus LT22, activate and culture at 36±1°C for 36 hours, then expand and culture at 35±1°C for 60 hours, during which the culture medium is shaken every 12 hours, The cell concentration obtained was 2.6 x 10 12 CFU / mL liquid Lactobacillus rhamnosus preparation;

[0052] (2) Preparation of Enterococcus faecalis preparation: take Enterococcus faecalis CH13, activate and culture at 36±1°C for 36 hours, then expand and culture at 35±1°C for 48 hours, during which the medium is shaken every 12 hours to obtain the bacterial concentration 3.5 x 10 11 CFU / mL liquid Enterococcus faecalis preparation;

[0053] (3) Preparation of Lactobacillus preparation: take Bacillus spp. SC27, activate it at 36±1°C for 24 hours, then expand it at 36±1°C and 150r / min on a shaker for 60 hours,...

Embodiment 2

[0059] The preparation of the solid dosage form of compound probiotic preparation for pigs, the steps are as follows:

[0060] The preparation method of liquid Lactobacillus rhamnosus preparation, liquid Enterococcus faecalis preparation, liquid Lactobacillus preparation, liquid Bacillus cereus preparation, liquid Bacillus subtilis preparation and liquid brewer's yeast preparation is the same as in Example 1;

[0061] (1) Preparation of Lactobacillus rhamnosus preparation: mix the liquid Lactobacillus rhamnosus preparation with a freeze-drying protectant at a weight ratio of 1:1, and freeze-dry at low temperature to obtain Lactobacillus rhamnosus powder; Lactobacillus rhamnosus bacteria powder is mixed with bran, and the weight ratio of the two is 1:10 to obtain a solid Lactobacillus rhamnosus preparation, and the number of bacteria is 3.2 x 10 10 CFU / g;

[0062] (2) Preparation of Enterococcus faecalis preparation: Mix the liquid Enterococcus faecalis preparation with the l...

Embodiment 3

[0069] Effects of complex probiotic preparations for pigs on the performance of pregnant sows:

[0070] 18 binary hybrid sows with basically the same gestation time were selected and divided into three groups, with 2 replicates in each group and 3 sows in each replicate; the sows in the control group were fed with basic diet; the experimental group and the experimental group 2 The sows started to be fed the basal ration supplemented with liquid formulations of complex probiotic preparations for pigs one month before farrowing, with a dose of 2mL / head and 4mL / head;

[0071] Compared with the control group, the test group 1 and test group 2 had basically the same total litter size, but the total number of stillborn, deformed, mummified piglets and weak piglets decreased by 36.36% and 54.55% compared with the control group, and the average weight of piglets born was lower than that of the control group. group increased by 5.38% and 10%, the results are shown in Table 1;

[0072]...

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PUM

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Abstract

The invention relates to porcine complex probiotics preparations and preparation method thereof. The porcine complex probiotics preparations comprise the components, by weight, of 20-30 parts of lactobacillus casei rhamnosus preparation, 15-25 parts of enterococcus faecalis preparations, 8-18 parts of bacillus coagulans preparations, 7-17 parts of bacillus cereus preparations, 10-20 parts of bacillus subtilis preparations, and 10-20 parts of saccharomyces cerevisiae preparations. Bacterial strains for preparing the components are lactobacillus casei rhamnosus LT22, enterococcus faecalis CH13, bacillus SC27, bacillus cereus SL1, bacillus subtilis and saccharomyces cerevisiae. The components are prepared through the technologies of activating cultivation, expanding cultivation, freeze drying, bran absorption and the like, and then the components are evenly mixed in proportion to obtain the porcine complex probiotics preparation, and the porcine complex probiotics preparations are divided into liquid form and solid form. The preparations contain high-activity polystrain probiotics, the probiotics has high cellular immunity and rich metabolic products, the porcine complex probiotics preparations have nutrition, immunization and probiotic effects to pigs, in particular pregnant sows and weanling pigs.

Description

technical field [0001] The invention relates to a microbial feed additive, in particular to a compound probiotic preparation for pigs and a preparation method thereof, belonging to the technical field of microecological preparations and feed additives. Background technique [0002] Since the 20th century, antibiotics and antibacterial drugs have been widely used in animal husbandry, but drug residues, drug resistance, reduced animal disease resistance and reduced quality of animal products caused by long-term use have become a serious and urgent problem to be solved. Countries around the world have adopted official compulsory measures to strictly limit the types, methods of use, and doses of antibiotics used in feed. Many countries have even banned the use of antibiotics in animal breeding and feed. [0003] Probiotic preparation is a kind of microbial preparation that has risen rapidly at home and abroad in recent years. As a new type of feed additive, it has no toxic and s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/00
Inventor 徐春厚刘颖谢为天赵生才邝雪梅
Owner GUANGDONG OCEAN UNIVERSITY
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