Application of Arabidopsis gene MYB73 on the aspect of plant sclerotinia sclerotiorum proof disease

Abstract

The sclerotinia sclerotiorum is an important disease of commercial crops, such as soybean and rape to cause the production reduction even total crop failure of the crop so as to cause severe economic loss. A sclerotinia sclerotiorum sensitive mutant myb73 for a crucifer plant disease is obtained by screening, the over-expression vector of a MYB73 gene (of which the gene number is AT4G37260) is built, and the over-expression mutant of the gene is obtained by agrobacterium conversion. An experiment shows that the MYB73 gene is induced and expressed by pathogenic bacteria to negatively regulate the expression of the in vivo anti-disease gene of the plant, the generation of the in vivo callose of the plant is regulated, and a theory and production practice foundation is laid for obtaining the new species of the sclerotinia sclerotiorum proof transgene plant by the analysis and the identification of the MYB73 gene function.

Description

technical field [0001] The present invention relates to the application of a kind of Arabidopsis gene MYB73 in anti-sclerotinia sclerotiorum, in particular to the application of the gene MYB73 in anti-sclerotinia sclerotiorum (Sclerotinia sclerotiorum), and also relates to the application of the gene MYB73 in cultivating anti-sclerotinia transgene The application in plant varieties belongs to the field of genetic engineering. Background technique [0002] For a long time, the infection of pathogenic bacteria has been the main reason for the huge loss of crop yield. Instead of passively waiting for the infection of pathogenic bacteria, plants will produce a series of disease-resistant defense responses, such as synthesizing phytodefensins, producing hydrolytic enzymes and some antibacterial proteins to resist the invasion of pathogenic bacteria. Using molecular biology theory and technology to study the interaction mechanism between plants and pathogenic bacteria at the mole...

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Problems solved by technology

[0004]Existing studies have shown that inducing callose deposition in plants can stimulate the basic disease resistance response in plants (Clay et al, 2009), MYB73 gene is the MYB gene in plants One of the members of the transcription factor family, when the myb73 mutant is infected with Pst DC3000, the production of callose is significantly higher than that of the wild type, indicating that MYB73 can regulate the production of callose in plants, but its detailed mechanism has not been reported, so , to analyze the function of the MYB73 gene, and to study its relationship with disease resistance, has important theoretical basis and practical application value

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