Method for culturing and detecting high selenium-enriched spirulina platensis

A technique for spirulina platensis, cultivation method, applied in the directions of microorganism-based methods, biochemical equipment and methods, unicellular algae, etc.

Inactive Publication Date: 2013-01-16
ANHUI UNIVERSITY +1
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] However, the sources of organic selenium in the market are limited. At present, selenium-enriched yeast with high selenium content has been developed relatively maturely. Researches by Chen Fusheng and others have shown that selenium-enriched yeast The total selenium content is 1.895mg / g, but the nutritional value of the yeast itself is low, and the spirulina itself is very nutritious and has a strong tolerance to inorganic selenium, while selenium-enriched rice and selenium-enriched tea have reported selenium content It is 20-300μg / kg. At the same time, the price of health care products such as ganoderma lucidum spores on the market is relatively expensive, so it is urgent to develop low-cost, high nutritional value, high immunity and high selenium-enriched spirulina health products

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  • Method for culturing and detecting high selenium-enriched spirulina platensis

Examples

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Effect test

Embodiment 1

[0028] Example 1 : The cultivation method of high selenium-enriched Spirulina platensis: Spirulina platensis is at temperature 25 ℃, pH 8, optimal light intensity 2500 lx, 24h light every day, adopts Zarrouk culture medium, is placed in the 250ml triangular culture flask, Seeding density is OD 560 = 0.35, the inoculation volume is 150ml, placed in the light incubator, when OD 560 When the algae liquid OD is 0.7, add 13.5 mg of sodium selenite for the first time, 560 Add Na for the second time when it is 0.8 2 SeO 3 15mg, when algae liquid OD 560 Add Na for the third time when it is 0.9 2 SeO 3 15mg continued to culture until OD 560 At 1.2, collect selenium-enriched spirulina, culture period is 11d, wash spirulina 3 times with distilled water, and freeze-dry to obtain selenium-enriched spirulina powder.

Embodiment 2

[0029] Example 2 : The cultivation method of high selenium-enriched Spirulina platensis: Spirulina platensis is at temperature 20 ℃, pH 10, optimal light intensity 2000 lx, 24h light every day, adopts Zarrouk culture medium, is placed in the 250ml triangular culture flask, Seeding density is OD 560 = 0.25, the inoculation volume is 150ml, placed in the light incubator, when OD 560 Add Na for the first time when it is 0.8 2 SeO 3 13.5mg, when algae liquid OD 560 Add Na for the second time when it is 0.9 2 SeO 3 13.5mg, when algae liquid OD 560 Add Na for the third time when it is 1.0 2 SeO 3 15mg continued to culture until OD 560 Selenium-enriched spirulina was collected at 1.5, the culture period was 13 days, the spirulina was washed 3 times with distilled water, and freeze-dried to obtain selenium-enriched spirulina powder.

Embodiment 3

[0030] Example 3 : Total selenium detection method of high selenium-enriched Spirulina platensis: Take 0.5g of selenium-enriched Spirulina, add digestive juice (concentrated nitric acid: high hypochlorous acid = 4:1), shake well, leave it overnight, and place it on the electric heating plate Heat until the organic matter decomposes or the color deepens, add 1ml of digestive juice to continue digestion, control the temperature below 200 degrees, and repeat this until the digestive juice is clear and contains HClO 4White smoke comes out, continue to heat until the solution reaches 1ml, dilute it in a 25ml volumetric flask with 60% concentrated HCL, then add 0.5%NaBH 4 , total selenium content was measured by atomic absorption method.

[0031] The organic selenium detection method of high-selenium-enriched Spirulina platensis: take 0.5g of selenium-enriched Spirulina, add 10ml of distilled water, put it on a heating plate and heat it to boil for 20min, cool and centrifuge, save...

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Abstract

The invention discloses a method for culturing and detecting high selenium-enriched spirulina platensis. The culture method comprises the following steps of: illuminating and culturing at temperature of between 20 and 25 DEG C for 24 hours every day under the conditions that the pH value is 8 to 10, the illumination intensity is 2,000 to 2,500 lx; culturing in a triangular culture flask by using a Zarrouk culture solution under the condition that the inoculum density OD560 is 0.25 to 0.35; adding Na2SeO3 sequentially until the total selenium amount is 270 to 300 mg / L; and when the OD 560 is 1.2 to 1.5, collecting spirulina platensis, and freeze-drying to prepare the high selenium-enriched spirulina platensis. According to the method, the culture condition is easy to control; and the spirulina platensis is high in reproduction speed, short in growth period and low in cost, the total selenium content is 2.0 to 2.5 mg / g, the content of organic selenium is 68 to 80 percent, and the content of essential amino acid and immunity are improved greatly.

Description

Technical field [0001] The present invention involves the cultivation and methods of high -rich and blunt spiral alkoabol in the field of health food and medicine. Background technique [0002] Gravoleum is the most nutritious and comprehensive green food found so far, especially the high protein content, amino acid balance, and rich essential fatty acids (such as R a linolenic acid, etc.).One of the biological active substances can achieve about 20 % of the quality of algae, and has good antioxidant and anti -tumor effects. [0003] Selenium is one of the essential trace elements of the human body, and its physiological functions are mainly manifested in the antioxidant effects of selenium and selenase.my country has successfully controlled various diseases through the selenium supplementation of the crowd, such as Keshan disease, macromioxida, cardiovascular disease, liver disease and tumor.The use of biological transformation pathways to produce organic selenium with higher bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12G01N21/64C12R1/89
Inventor 黄蓓王鑫焱沈敬连武子涵刘亚华
Owner ANHUI UNIVERSITY
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