Method for extracting cellulase from microorganism fermentation broth
A microbial fermentation liquid and microbial fermentation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost, complicated operation, and short time-consuming cellulase, and achieve short time-consuming and simple operation , the effect of small energy consumption
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Embodiment 1
[0039] The fermented liquid submerged by Trichoderma reesei for 200 hours was first clarified through a plate and frame filter press equipped with 5 layers of gauze for primary clarification, and then through a 10 μm filter cloth for secondary clarification. .
[0040] Adjust the pH of the clarified fermentation broth to 4.0, and adjust the rotation speed to 200r / min. Weigh 3g / L tannin and fully dissolve it with pH4.0 citric acid buffer solution, then add it to the mixing tank at one time, stir it evenly and let it stand for 1h. The fermented broth after tannin precipitation was collected by press filtration with a 10 μm filter cloth plate frame, and the precipitate was collected by vacuum freeze dryer at a vacuum degree of 11 Pa and at -50°C for 30 hours to obtain a powdered cellulase product. The enzyme activity of the fermentation broth was measured by the international standard method recommended by the International Union of Theoretical and Applied Chemistry, and the yie...
Embodiment 2
[0042]The fermented liquid submerged by Trichoderma reesei for 200 hours was first clarified through a plate and frame filter press equipped with 5 layers of gauze for primary clarification, and then through a 10 μm filter cloth for secondary clarification.
[0043] Adjust the pH of the clarified fermentation broth to 4.5, and adjust the rotation speed to 150 r / min. Weigh 5g / L tannin and fully dissolve it with pH4.5 citric acid buffer solution, then add it to the stirring tank at one time, and keep stirring for 2h. The fermented broth after tannin precipitation was collected by press filtration with a 10 μm filter cloth plate frame, and the precipitate was collected by vacuum freeze dryer at a vacuum degree of 11 Pa and at -50°C for 30 hours to obtain a powdered cellulase product. The enzyme activity of the fermentation broth was measured by the international standard method recommended by the International Union of Theoretical and Applied Chemistry, and the yield of the enzym...
Embodiment 3
[0045] The fermented liquid submerged by Trichoderma reesei for 200 hours was first clarified through a plate and frame filter press equipped with 5 layers of gauze for primary clarification, and then through a 10 μm filter cloth for secondary clarification.
[0046] Adjust the pH of the clarified fermentation broth to 5.0, and adjust the rotation speed to 100 r / min. Weigh 7g / L tannin and fully dissolve it with pH5.0 citric acid buffer, then slowly and evenly add it to the stirring tank, and continue stirring for 4h. The fermented broth after tannin precipitation was collected by press filtration with a 10 μm filter cloth plate frame, and the precipitate was collected by vacuum freeze dryer at a vacuum degree of 11 Pa and at -50°C for 30 hours to obtain a powdered cellulase product. The enzyme activity of the fermentation broth was measured by the international standard method recommended by the International Union of Theoretical and Applied Chemistry, and the yield of the enz...
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