Fingerprint constructing method of total flavonoid components and total alkaloids components in loranthus parasiticus-kudzuvine root preparation and quality detecting method

A technology of fingerprint spectrum and total alkaloids, which is applied in the field of quality inspection of mulberry preparations, can solve the problems that the quality of mulberry preparations cannot be controlled, cannot fully reflect the content of total flavonoids and total alkaloids active ingredients, etc. Safety, good reproducibility, and the effect of protecting rights and interests

Active Publication Date: 2013-01-23
BEIJING WBL PEKING UNIV BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the above-mentioned existing detection methods of Sangge preparations cannot fully reflect the content of total flavonoids and total alkaloids active ingredients, and ca

Method used

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  • Fingerprint constructing method of total flavonoid components and total alkaloids components in loranthus parasiticus-kudzuvine root preparation and quality detecting method
  • Fingerprint constructing method of total flavonoid components and total alkaloids components in loranthus parasiticus-kudzuvine root preparation and quality detecting method
  • Fingerprint constructing method of total flavonoid components and total alkaloids components in loranthus parasiticus-kudzuvine root preparation and quality detecting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1 Construction of Sangge Preparation Total Flavonoids Control Fingerprint

[0090] The 10 batches of Morus alba medicinal materials and Pueraria radiata used in Example 1 are mainly produced in Henan, Hebei, Anhui, Guangxi, and Hunan. After identification, the above-mentioned varieties meet the pharmacopoeia standards and have no obvious biological differences. For details, please refer to Table 1:

[0091] Table 1

[0092]

[0093] Morus alba

[0094] Table 2 shows the specific batch numbers of Cortex Mori and Radix Puerariae in 10 batches of Sangge preparations.

[0095] Table 2

[0096] Sangge preparation number

[0097] Instruments used: SHIMADZU HPLC including LC-20AT chromatographic pump, SPD-M20A UV detector, CBM-20A controller, DGU-20A5 degasser, SIL-20A autosampler, CTO-10AS column thermostat , class-vp workstation; chromatographic column: Apollo-C18 (250×4.6mm, 5μm) analytical column.

[0098] Preparation of reference s...

Embodiment 2

[0124] Embodiment 2 is to the quality detection method of total flavonoids composition in the Sangge preparation

[0125] The source of the Sangge preparation to be tested:

[0126] From Morus alba extract S20100721 ​​(extracted from Morus alba medicinal material with batch number Y070609HN) and kudzu root extract G20100813 (extracted from kudzu root medicinal material with batch number G110118HN) according to the ratio of medicinal materials 2:1, 4:1, 1 :2, 1:4 mixed.

[0127] The preparation and chromatographic conditions of the instrument used, the reference substance solution, the Sangge preparation solution to be tested are all the same as in Example 1.

[0128] Precisely draw 10 μl each of the reference substance solution and the Sangge preparation solution to be tested, inject into the liquid chromatograph, measure, and record the chromatogram for 70 minutes. For the fingerprints of the total flavonoid components of the Sangge preparation to be tested, please see the ...

Embodiment 3

[0130] Example 3 Construction of fingerprints of total alkaloid components in Sangge preparations

[0131] Instruments used: SHIMADZU HPLC including LC-20AT chromatographic pump, RF-20A fluorescence detector, CBM-20A controller, DGU-20A5 degasser, SIL-20A autosampler, CTO-10AS column thermostat , class-vp workstation; chromatographic column: CosmosilC18 (250×4.6mm, 5μm) analytical column

[0132] Preparation of reference solution: Accurately weigh an appropriate amount of 1-deoxynojirimycin hydrochloride reference substance (Sigma Company, batch number 078K4062), add water to make a solution containing 4 μg per 1 ml;

[0133] 1) Preparation of Sangge preparation solution: each batch of Sangge preparations was prepared according to the mass ratio of Morus alba and Pueraria 2:1, 4:1, 1:2, and 1:4 to prepare four Sangge preparations . Take by weighing about 0.05g of each of the above Sangge preparations, accurately weigh, put in a 100ml conical flask with a stopper, accurately ad...

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Abstract

The invention provides a fingerprint constructing method of total flavonoid components and total alkaloids components in loranthus parasiticus-kudzuvine root preparation and a quality detecting method of the loranthus parasiticus-kudzuvine root preparation. The fingerprint constructing method comprises the steps as follows: a) preparing loranthus parasiticus-kudzuvine root preparation solution; b) constructing a fingerprint of total flavonoid components; and c) constructing a fingerprint of total alkaloids components. With the adoption of the constructing method provided by the invention, the fingerprints of the total flavonoid components and total alkaloids components can be obtained and are used for controlling the quality of the loranthus parasiticus-kudzuvine root preparation. With the adoption of the constructing method and the detecting method, the standard in quality control of the loranthus parasiticus-kudzuvine root preparation is improved, the content conditions of the total flavonoid components and the total alkaloids components are relatively comprehensively shown, and moreover, the advantages of being high in stability and high in repeatability are realized.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicinal materials, in particular to a method for constructing fingerprints of total flavonoid components and total alkaloid components in a mulberry preparation and a quality detection method for a mulberry preparation. Background technique [0002] Morus alba L. is the dry root bark of Morusalba L., a plant of the Moraceae family. It is sweet and cold in nature and returns to the lung meridian. It has the functions of purging the lungs, relieving asthma, diuresis and reducing swelling. edema. Pueraria lobata (Willd) Ohwi is the dried root of the perennial vine of the leguminous family Pueraria lobata (Willd) Ohwi. It is a commonly used medicine in traditional Chinese medicine. [0003] In the prior art, people mixed the two medicinal materials of Cortex Mori and Pueraria lobata to prepare a Sangge preparation, and applied it to the preparation of traditional Chinese medi...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 段震文郭树仁薛岚
Owner BEIJING WBL PEKING UNIV BIOTECH
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