Fluid device for controlling microtubule movement direction, its preparation method and application

A micropipe movement and fluid technology, applied in the manufacture of microstructure devices, microstructure devices, decorative art, etc., can solve the problems of increased research and development costs, high cost, no directionality, etc., to achieve easy control, low cost, and easy movement direction effect

Inactive Publication Date: 2015-07-22
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the movement between microtubules and motor kinesins presents a corresponding relationship, in the absence of external control, microtubules exhibit disordered and non-directional movement characteristics on the surface of motor kinesin-modified substrates, so , in order to efficiently use the microtubule-motor protein system in micro-nano devices, it is necessary to artificially control the direction of microtubule movement
At present, the commonly used technology is to directly use silicon wafers with micro-nano arrays to prepare microfluidic devices, but the cost of this method is too expensive, which greatly increases the cost of research and development

Method used

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  • Fluid device for controlling microtubule movement direction, its preparation method and application
  • Fluid device for controlling microtubule movement direction, its preparation method and application
  • Fluid device for controlling microtubule movement direction, its preparation method and application

Examples

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Embodiment 1

[0035] This embodiment is used to illustrate the fluid device and its preparation method for controlling the movement direction of microtubules provided by the present invention, and the specific details are as follows:

[0036] (1) Preparation of polydimethylsiloxane (PDMS) stamp:

[0037] The silicon template with the micro-nano array in the present invention comes from the Institute of Physics, Chinese Academy of Sciences, and is prepared by a traditional photolithography process. Using the silicon template as a bottom mold, a polydimethylsiloxane stamp was prepared by soft etching. The silicon template has a generally flat surface with a series of parallel grooves that are used to mold the protrusions on the polydimethylsiloxane stamp.

[0038] Silanize and hydrophobicize the silicon template, that is, immerse the silicon template in a toluene solution of 1mM octadecyltrichlorosilane for 2h, rinse with toluene, blow dry with nitrogen, and bake at 120°C for 2h to make the ...

Embodiment 2

[0044] This embodiment is used to illustrate the fluidic device and its preparation method for controlling the movement direction of microtubules provided by the present invention.

[0045] The experimental steps and parameters of this example are basically the same as those in Example 1, the difference is that a casein solution with a concentration of 1 mg / mL is passed into each microfluidic channel for pre-modification, and then a motor with a concentration of 50 nM is passed through. A kinesin solution, the motor kinesin in the motor kinesin solution is prepared by transforming and expressing an exogenous plasmid in Escherichia coli.

[0046] The fluid device for controlling the movement direction of microtubules prepared by the above steps is as follows: figure 1 shown.

Embodiment 3

[0048] This example is used to illustrate the method of using the fluid device for controlling the direction of movement of microtubules provided by the present invention, or using the fluid device prepared by the preparation method provided by the present invention, for controlling the direction of movement of microtubules. The specific details are as follows :

[0049] (1) Using the preparation method provided in Example 1, a fluidic device for controlling the movement direction of microtubules was prepared.

[0050] (2) Prepare a microtube solution containing oxygen scavenger and ATP. The concentration of the microtube is 4 μg / mL. The oxygen scavenger contains 10 mM glucose, 10 μg / mL glucose oxidase, 4 μg / mL catalase and 0.5 wt% β - Mercaptoethanol, the concentration of ATP is 0.5 mM. In order to make microtubules produce fluorescence for easy observation, microtubules were polymerized by mixing rhodamine-labeled tubulin and unlabeled tubulin at a ratio of 1:4.

[0051] (...

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Abstract

The invention provides a micro-nanofluid device, its preparation method and application. The device provided by the invention comprises a glass layer and a polydimethylsiloxane seal. The polydimethylsiloxane seal is composed of a substrate and a plurality of protrusions formed with the substrate integrally. The glass layer is attached to the top surfaces of the plurality of protrusions of the polydimethylsiloxane seal so as to form multiple microfluidic channels, wherein preferably, the plurality of protrusions are parallel or not parallel to each other in a longitudinal extending direction. Meanwhile, the invention provides a preparation method of the micro-nanofluid device. The method consists of: (1) preparing the polydimethylsiloxane seal; and (2) attaching the glass layer to the top surfaces of the plurality of protrusions of the polydimethylsiloxane seal prepared in step (1), thus forming the multiple microfluidic channels. The device can be used to control the microtubule movement direction.

Description

technical field [0001] The invention relates to a fluid device, in particular to a fluid device which can be used to control the movement direction of microtubes, its preparation method and application. Background technique [0002] Motor kinesin (kinesin) is a kind of protein distributed inside or on the cell surface. Its conformation will change with the alternating combination with ATP and ADP. The energy of ATP hydrolysis is converted into mechanical energy, causing the deformation of motor kinesin, or with it. The combined molecules move. [0003] As the self-assembly building block of the bionic system, the high energy conversion efficiency and complex movement mode of the motor kinesin indicate that its evolution has reached a very high level. It can convert the hydrolysis energy of ATP into mechanical energy, and the conversion rate has reached 50%. , twice the conversion rate of human-designed machines (see Kural, C., et al. Kinesin and Dynein Move a Peroxisome in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B81C1/00
Inventor 李峻柏宋卫星
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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