Preparation method and application of chitosan nanoparticles with pore structures

A technology of chitosan nanoparticles and pore structure, which is applied in the direction of medical preparations of non-active ingredients, pharmaceutical formulas, powder transportation, etc., and can solve product processing difficulties, low concentration of chitosan solution, low production efficiency of nanoparticles, etc. problem, to achieve the effect of high production efficiency

Inactive Publication Date: 2013-02-13
JIANGSU UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] At present, the methods for preparing chitosan nanoparticles mainly include ion gel method and reverse microemulsion method, wherein the preparation conditions of ion gel method are mild, but the chitosan used The sugar solution concentration must be very low, resulting in a low yield of nanoparticles, and the stability of the obtained chitosan nanoparticles is also poor; while the inverse microemulsion method requires a large amount of surfactants and co-surfactants, the carrier material Concentration and dispersed phase / continuous phase volume ratio must also be very low to form a microemulsion, resulting in difficulties in product post-processing and low production efficiency of nanoparticles
There are also very few chitosan nanoparticles prepared by the water-in-oil miniemulsion method, but the volume ratio of the oil phase to the water phase is still as high as 9:1 (Yong-Zhong Dua, et al. International Journal of Pharmaceutics. 392: 164–169 , 2010)

Method used

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  • Preparation method and application of chitosan nanoparticles with pore structures
  • Preparation method and application of chitosan nanoparticles with pore structures
  • Preparation method and application of chitosan nanoparticles with pore structures

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Weigh 770 mg of chitosan with a molecular weight of 55,000 Daltons and a degree of deacetylation of 90%, dissolve it in 30 mL of 1.5% acetic acid solution to obtain a 2.5% chitosan solution; dissolve 1.0 g of Span-80 in 38.6 g liquid paraffin and n-hexane mixed solvent (V 液体石蜡 :V 正己烷 = 4:1) to obtain the oil phase; add the chitosan solution to the oil phase, shear emulsify at 8000rpm for 15min, transfer the obtained miniemulsion into a three-necked flask, stir at 400rpm, and slowly add pentadiol dropwise Aldehyde ether solution (V 25%戊二醛溶液 :V 乙醚 =1:2) 3mL, react at 40°C for 4h, keep the stirring speed at 400rpm, use a circulating water vacuum pump to depressurize and dehydrate, and at the same time increase the system temperature from 40→70°C at a speed of 1°C / min, when there is no water in the condenser tube When flowing out, the obtained chitosan suspension was centrifuged at 8000 rpm for 30 minutes to separate the nanoparticles, the obtained nanoparticles were was...

Embodiment 2

[0033] Weigh 460 mg of chitosan with a molecular weight of 150,000 Daltons and a degree of deacetylation of 78%, dissolve it in 30 mL of 1.0% acetic acid solution to obtain a 1.5% chitosan solution; dissolve 1.2 g of Span-80 in 39.0 g liquid paraffin and n-hexane mixed solvent (V 液体石蜡 :V 正己烷 = 4:1) to obtain the oil phase; add the chitosan solution into the oil phase, shear and emulsify at a rate of 10000rpm for 10min, transfer the resulting miniemulsion into a three-necked flask, stir at 500rpm, and slowly add pentadiene Aldehyde ether solution (V 25%戊二醛溶液 :V 乙醚 =1:2) 2mL, react at 50°C for 3h, lower the temperature of the system to 40°C, keep the stirring speed at 500rpm, depressurize and dehydrate the system with a circulating water vacuum pump, and at the same time increase the system temperature from 40→70°C at a speed of 1°C / min , when no water flows out from the condenser tube, the resulting chitosan suspension is centrifuged at a rate of 10,000 rpm for 40 min to sep...

Embodiment 3

[0035] Weigh 300 mg of chitosan with a molecular weight of 210,000 Daltons and a degree of deacetylation of 95%, dissolve it in 30 mL of 1.0% acetic acid solution to obtain a 1.0% chitosan solution; dissolve 1.3 g of Span-80 in 38.8 g liquid paraffin and n-hexane mixed solvent (V 液体石蜡 :V 正己烷 =4:1) to obtain the oil phase; add the chitosan solution into the oil phase, shear emulsify at 12000rpm for 12min, transfer the resulting miniemulsion into a three-necked flask, stir at 500rpm, and slowly add pentadiene Aldehyde ether solution (V 25%戊二醛溶液 :V 乙醚 =1:2) 2mL, react at 60°C for 2h, lower the temperature of the system to 40°C, keep the stirring speed at 500rpm, depressurize and dehydrate the system with a circulating water vacuum pump, and at the same time increase the system temperature from 40→70°C at a speed of 2°C / min , when no water flows out from the condenser tube, the resulting chitosan suspension is centrifuged at a rate of 12000 rpm for 40 min to separate the nanopa...

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Abstract

The invention belongs to the technical field of nanometer material preparation, and relates to a preparation method of chitosan nanoparticles, in particular to a preparation method and an application of the chitosan nanoparticles with pore structures. According to the preparation method and the application of the chitosan nanoparticles with the pore structures, first, chitosan is dissolved into acetic acid solution, and chitosan solution is obtained; second, span-80 is dissolved into organic solvent to obtain oil phase, third, the chitosan solution and the oil phase is cut and emulsified in a high speed, and opposite phase miniemulsion is obtained; the opposite phase miniemulsion is stirred and cross linker solution is dropped into the opposite phase miniemulsion to react; and then a system is decompressed, dehydrated, centrifugated, washed and vacuum dryed, so that the chitosan nanoparticles with the pore structures are obtained. According to the preparation method and the application of the chitosan nanoparticles with the pore structures, the grain diameters of the chitosan nanoparticles with the pore structures are ranged from 100 nm - 500 nm, and the chitisan nanoparticles with the pore structures are good carriers of medicines, and can be used for medicine absorption. Meanwhile, in preparation process, water phase and the oil phase inside the opposite phase miniemulsion are similar, so that the production efficiency of nanoparticles is high.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation, relates to a preparation method of chitosan nanoparticles, in particular to a preparation method and application of chitosan nanoparticles with a pore structure. Background technique [0002] Chitosan is the product of N-deacetylation of chitin. Generally speaking, chitosan can be called chitosan if more than 60% of the N-acetyl group has been removed. As a positively charged natural polysaccharide, chitosan itself has properties such as non-toxic, non-irritating, non-allergenic, and non-mutagenic. The degradation products are low-molecular chitosan and glucosamine, which have good biological properties. Compatibility and biodegradability. Chitosan is prepared into nano-microspheres or microparticles as a carrier for drug delivery and controlled release, which has the characteristics of high drug encapsulation efficiency and strong stability. [0003] At present, the methods fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K9/14C08J3/12
Inventor 李夜平程晓农黄立英徐云
Owner JIANGSU UNIV
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