The invention belongs to the technical field of nanometer material preparation, and relates to a preparation method of
chitosan nanoparticles, in particular to a preparation method and an application of the
chitosan nanoparticles with pore structures. According to the preparation method and the application of the
chitosan nanoparticles with the pore structures, first, chitosan is dissolved into
acetic acid solution, and chitosan solution is obtained; second, span-80 is dissolved into
organic solvent to obtain
oil phase, third, the chitosan solution and the
oil phase is
cut and emulsified in a high speed, and opposite phase
miniemulsion is obtained; the opposite phase
miniemulsion is stirred and
cross linker solution is dropped into the opposite phase
miniemulsion to react; and then a
system is decompressed, dehydrated, centrifugated, washed and vacuum dryed, so that the
chitosan nanoparticles with the pore structures are obtained. According to the preparation method and the application of the
chitosan nanoparticles with the pore structures, the grain diameters of the
chitosan nanoparticles with the pore structures are ranged from 100 nm - 500 nm, and the chitisan nanoparticles with the pore structures are good carriers of medicines, and can be used for
medicine absorption. Meanwhile, in preparation process, water phase and the
oil phase inside the opposite phase miniemulsion are similar, so that the production efficiency of nanoparticles is high.