The invention discloses a cobweb-shaped flexible strain sensor capable of identifying a strain direction and a preparation method. The method comprises the following steps of mixing a conductive filler, a hot-melt adhesive and an N, N-dimethylformamide solvent, and carrying out ultrasonic dispersion to obtain a dispersion liquid, putting the granular thermoplastic polymer into a screw extruder, and extruding a pure thermoplastic polymer wire, drawing the wire material to pass through the dispersion liquid at a constant speed by utilizing a wire winding roller traction device, forming a uniformand thin conductive layer on the surface of the wire material, and printing the wire material into a cobweb-shaped array by utilizing a 3D printer after the wire material is completely dried, and fixing the wire at the center of the cobweb and the tail end of the longitudinal wire by using conductive silver adhesive to prepare the cobweb-shaped flexible strain sensor. The cobweb-shaped customizedstructure is rapidly prepared, and the sensor also has the function of identifying the strain direction, has the advantages of high linearity, high sensitivity, wide strain range, rapid response, lowhysteresis, good stability and the like, and has great potential in the application of wearable equipment.