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Maackia amurensis total flavone extract, as well as preparation method and application thereof

A technology of extract and total flavonoids, applied in the field of medicine

Inactive Publication Date: 2013-02-20
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the fact that there is no commercially available total flavonoid extract of Sophora japonica, so developing a simple and feasible method to obtain the total flavonoid extract of Sophora japonica for the preparation of estrogenic drugs or health food can fill the gaps in the art about Sophora japonica Blanks in extraction and application of total flavonoids

Method used

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  • Maackia amurensis total flavone extract, as well as preparation method and application thereof
  • Maackia amurensis total flavone extract, as well as preparation method and application thereof
  • Maackia amurensis total flavone extract, as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0023] Weigh about 1.0 kg of dry locust bark powder (passed through a 24-mesh sieve), add 30L of 60% ethanol and soak for 30 minutes, then reflux and extract 3 times, each for 1 hour. The extracts were combined, filtered, and concentrated under reduced pressure. The concentrated solution is dissolved in water, filtered, passed through D-101 macroporous adsorption resin, washed with 5 times the column bed volume of water, 4 times the column bed volume is eluted with 10% ethanol, and 6 times the column bed volume is eluted with 60% ethanol. , Combine the 60% ethanol eluate, concentrate to dryness, and get it. With the main ingredient Saikoisoflavonoside A as the reference substance, the content of total flavonoids in the extracted extract was calculated to be 53% by UV spectrophotometry, and the content of orrisin and Saikoisoflavonoside A in the extract detected by HPLC were the extracts. Of 4.5% and 24.2%.

Embodiment 2

[0025] Weigh about 1.0 kg of dry locust bark powder (passed through a 50-mesh sieve), add 15L of 50% ethanol and soak for 30 minutes, then reflux and extract 4 times, each for 1.5 hours. The extracts were combined, filtered, and concentrated under reduced pressure. The concentrated solution is dissolved in water, filtered, passed through ADS-7 macroporous adsorption resin, washed with 10 times the column bed volume of water, 8 times the column bed volume is eluted with 50% ethanol, combined the 50% ethanol eluate, and concentrated to dryness. Immediately. With the main ingredient Saikoisoflavonoside A as the reference substance, the content of total flavonoids in the extract was calculated to be 38% by UV spectrophotometry, and the content of irisin and Saikoisoflavonoside A in the extract detected by HPLC were the extracts. 3.4% and 16.7% of the total.

Embodiment 3

[0027] Weigh about 1.0 kg of dry locust bark powder (passed through a 65-mesh sieve), add 20L of 70% ethanol and soak for 30 minutes, reflux and extract 3 times, each for 1.5 hours. The extracts were combined, filtered, and concentrated under reduced pressure. The concentrated solution is dissolved in water, filtered, added to the AB-8 macroporous resin column for saturated adsorption, washed with 5 times the column bed volume of water, and then eluted with 4 times the column bed body 20% ethanol to remove impurities, and then used 5 Elute with 70% ethanol from the double column bed, combine the 70% ethanol eluate, and concentrate to dryness to obtain. Taking the main ingredient Saikoisoflavonoside A as a reference substance, the content of total flavonoids in the extract was calculated to be 82% by UV spectrophotometry, and the content of irisin and Saikoisoflavonoside A in the extract detected by HPLC was the same as that of the extract. 9.4% and 54.5%.

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Abstract

The invention relates to a maackia amurensis total flavone extract as well as a preparation method and an application thereof. The maackia amurensis total flavone extract is prepared through the method which comprises the following steps: extracting the heartwood, velamen, bark, twig bark, leaves, stalks, flowers, fruits and seeds of maackia amurensis by utilizing an extraction solvent; filtering and concentrating the extracting solution; passing the concentrated solution through the macroporous adsorption resin, and eluting by utilizing methanol or ethanol water of different concentrations; and collecting and concentrating the eluant until the solution is dry, so that the maackia amurensis total flavone extract is obtained. The maackia amurensis total flavone extract which is obtained through the method contains the total flavone content of 15%- 90%, tectoridin of 1-15% and SaikoisoflavonosideA of 6-57%. The maackia amurensis total flavone extract has an estrogen effect, can be used for preparing estrogen drugs or health-care food and is used for preventing or treating breast cancer, prostate cancer, colon cancer, osteoporosis and climacteric syndrome.

Description

Technical field [0001] The invention belongs to the field of medical technology. The invention relates to a preparation method and application of total flavonoids of Korean locust, in particular to a preparation method of total flavonoids of Korean locust and application in the preparation of estrogen drugs or health foods. Background technique [0002] Korean Sophora ( Maackia amurensis Rupr. et Maxim.), also known as locust tree, Korean locust tree, and Huai locust tree, belong to the leguminous saddle tree. The whole plant of Pseudoacacia koraiensis contains a variety of flavonoid compounds formononetin (formoononetin), genistein (genistein), afromosin (afromosin), daidzein (afromosin), orris aglycone, kudzu flower palatine, etc. Its glycosides are one of the few plants that contain 4 kinds of isoflavones (phytoestrogens). [0003] Epidemiological studies have found that the incidence of breast cancer in women is related to the content of phytoestrogens in their diet. Reside...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P15/12A61P19/10A61P35/00A61K125/00A61K127/00A61K131/00A61K133/00A61K135/00
Inventor 王东尹涛吕重宁刘东春夏明钰崔征代英辉
Owner SHENYANG PHARMA UNIVERSITY
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