Modification method for improving bacteria heavy metal adsorption capacity, adsorbent and application thereof

A technology of adsorption capacity and heavy metals, applied in the field of heavy metal water pollution remediation, can solve the problem of high cost, achieve the effects of simple operation, outstanding heavy metal removal effect, and simple modification method

Inactive Publication Date: 2013-03-13
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some traditional treatment methods such as chemical precipitation, ion exchange, membrane technology, electrochemical treatment, activated carbon adsorption, etc., but these methods have problems in the pro

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  • Modification method for improving bacteria heavy metal adsorption capacity, adsorbent and application thereof
  • Modification method for improving bacteria heavy metal adsorption capacity, adsorbent and application thereof
  • Modification method for improving bacteria heavy metal adsorption capacity, adsorbent and application thereof

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Embodiment 1

[0025] 1, the preparation of adsorbent of the present invention:

[0026] (1) Prepare unmodified plant endophyte Pseudomonas sp.LK9 cells

[0027] First, a large amount of endophyte Pseudomonas sp.LK912h was cultivated with LB medium, the cells were collected by centrifugation, washed 2-3 times with deionized water, and freeze-dried powder of endophyte Pseudomonas sp.LK9 was obtained by freeze-drying.

[0028] (2) Modified endophyte Pseudomonas sp.LK9 cells

[0029] Firstly, the influence of glutaraldehyde concentration on the effect of PAA modification on LK9 was discussed: 5g / L PAA / HCl solution was prepared, its pH was adjusted to 9-12, and the lyophilized powder of endophyte Pseudomonas sp. Add propylamine hydrochloride solution to PAA / HCl alkaline solution, stir for 2 hours, add 25% glutaraldehyde in different volumes to make the glutaraldehyde content 0.25%, 0.375%, 0.5%, 1%, 1.5% %, 2%, continue to stir for 2h, wash with deionized water twice, freeze-dry to constant we...

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Abstract

The invention provides a modification method for improving bacteria heavy metal adsorption capacity, an adsorbent and the application of the method. The method takes glutaraldehyde as a cross-linking agent, and comprises the step of: connecting poly allyamine hydrochloride (PAA/ HCl) to the surface of plant endophyte Pseudom onas sp. LK9 thallus so as to cause the surface of the thallus to have a great deal of amino groups and further improve the adsorption effect of the thallus for heavy metals. The maximum adsorbing capacities of the obtained adsorbent for the heavy metals Pb<2+>, Cd<2+> and Cu<2+> are respectively 269.9mg/ g, 145.0mg/ g and 148.0mg/ g; and compared with an LK9 adsorbent which is not modified, the adsorbing capacities are respectively increased by 192.98mg/ g, 121.85mg/ g and 128.35mg/ g. The invention has the characteristics of being simple in method, simple and convenient to operate, remarkable and rapid in adsorption effect, environment-friendly and the like, thus having great application potential in the aspect of heavy metal pollution water treatment.

Description

Technical field: [0001] The invention relates to the field of remediation of heavy metal water pollution, in particular to a method for repairing functional groups on the surface of bacterial cells. The bacterial adsorbent prepared from the modified cells can quickly and efficiently remove heavy metals in water. Background technique: [0002] As we all know, water resources are one of the most important resources for biological survival. However, with the rapid development of industry, water pollution is becoming more and more serious. Among them, heavy metal pollution is one of the most serious problems of water pollution. Heavy metal water pollution mainly comes from the discharge of various industrial wastewater wastes, such as mining, metallurgy, smelting, electroplating, and ceramics, electrical appliances, pesticide production, etc. During these industrial production processes, a large amount of water is discharged into the water body Toxic and harmful heavy metal sub...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C02F3/34C12R1/38C02F101/20
Inventor 罗胜联李晓洁陈亮万勇陈觉梁刘承斌
Owner HUNAN UNIV
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