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Method for measuring cordycepin, adenosine and mannitol in cordyceps sinensis mycelium powder

A technology of Trichosporium and cordycepin, applied in adenosine, determination of cordycepin and mannitol in the mycelial powder of Trichospora bat moth, can solve the problem of low sensitivity, long time, and inability to detect cordycepin, Adenosine, mannitol and other problems, to achieve the effect of fast analysis time, low detection limit and strong separation ability

Inactive Publication Date: 2013-03-13
黄宏南
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Problems solved by technology

Huang Guangyu et al. used LC / ESI-MS method to measure the content of adenosine in Cordyceps sinensis with 2-chloroadenosine as internal standard (Huang LF, Liang YZ, Guo FQ, Zhou ZF, Cheng BM. Simultaneous separation and determination of active components in Cordyceps sinensis and Cordyceps militarris by LC / ESI-MS. J Pharm Biomed Anal 2003 Dec 4;33(5):1155-62.), but this method can only measure one component at a time, not simultaneously Detection of cordycepin, adenosine and mannitol takes a long time and the sensitivity is not high

Method used

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  • Method for measuring cordycepin, adenosine and mannitol in cordyceps sinensis mycelium powder
  • Method for measuring cordycepin, adenosine and mannitol in cordyceps sinensis mycelium powder
  • Method for measuring cordycepin, adenosine and mannitol in cordyceps sinensis mycelium powder

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Embodiment Construction

[0025] 1. Experimental instruments and materials

[0026] (1) Instrument: High Performance Liquid Chromatography-Ion Trap Mass Spectrometer produced by Thermo Electric Corporation, model LCQ-Deca-XP, equipped with an LC system (Thermo Finnigan, San Jose, CA, U.S.A.), including quaternary Pump, online degasser, column heater section, autosampler and PDA (photodiode array) detector and multi-stage ion trap mass spectrometer (Thermo Finnigan), which consists of an ESI interface and an ion trap mass spectrometer, data Process and obtain through Xcalibur 1.3 workstation; Milli-Q ultrapure water system ((Millipore Inc, Billerica, MA, U.S.A.); electronic balance, high-speed centrifuge, ultrasonic instrument;

[0027] (2) Reagents: methanol (HPLC grade, Merck, Darmstadt, Germany); formic acid (analytical grade, Merck, Darmstadt, Germany); cordycepin standard (purity ≥ 99%), adenosine standard (purity ≥ 99%) , Mannitol standard product (purity ≥ 99%), Cordyceps sinensis mycelium powde...

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Abstract

The invention relates to a method for measuring cordycepin, adenosine and mannitol in cordyceps sinensis mycelium powder, and in particular relates to a method for simultaneously measuring cordycepin, adenosine and mannitol in cordyceps sinensis mycelium powder by using an efficient liquid chromatograph-mass spectrometer. The method comprises the steps of: pretreating a sample and a standard product; optimizing conditions of the efficient liquid chromatograph-mass spectrometer; measuring the sample; analyzing data; and finally determining a mobile phase as a formic acid-methanol mixture (94:6, v / v), wherein a chromatographic column is Thermo BioBasic-18PIONEER (150mm*2.1mm, i.d. 5mu m), a selected ion monitoring (SIM) mode is adopted for qualitative and quantitative analyses on the three active components. The method integrates the advantages of chromatogram and mass spectrum, is simple and quick in measuring process, accurate in measuring result, high in flexibility, and suitable for analysis and detection on the cordycepin, the adenosine and the mannitol in the cordyceps sinensis mycelium powder.

Description

technical field [0001] The invention relates to a method for determining cordycepin, adenosine and mannitol in the mycelia powder of Trichosporum spp. Methods for the content of cordycepin, adenosine, and mannitol. Background technique [0002] Cordyceps sinensis is a parasitic fungus, scientifically known as Trichosporum batata, parasitic on the larvae of the order Lepidoptera that grow at high altitudes, and has been used as food and herbal medicine in China to treat various diseases. It can not only be used as a precious medicine to treat many diseases, such as cough, lung and kidney dysfunction, but also as an anti-aging health tonic according to recent reports. Cordyceps sinensis has been proven to contain major bioactive components such as cordycepin, adenosine, mannitol, inosine, adenine, and cordycepin polysaccharide, which have the effects of anti-oxidation, enhancing immunity, and regulating the human endocrine system, especially the three most important Active i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 黄宏南
Owner 黄宏南
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