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Universal nanoscale electrospining regionalization method

An electrospinning, regionalized technology, applied in biochemical equipment and methods, textiles and papermaking, filament/thread forming, etc., can solve problems such as low flux, inability to regionalize electrospinning, and single research conditions. Achieve the effect of controllable area, reduced screening cost and simple operation

Active Publication Date: 2013-03-20
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a general-purpose method for regionalization of nano-electrospinning, which solves the problems of traditional electrospinning that cannot be regionalized, single research conditions, and low flux, and provides research fields such as biology and regenerative medicine. a new platform

Method used

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  • Universal nanoscale electrospining regionalization method
  • Universal nanoscale electrospining regionalization method
  • Universal nanoscale electrospining regionalization method

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The electrospinning method can be used to fabricate electrospun arrays. according to figure 1 The process shown is for regionalization of electrospinning, such as using a clean substrate material glass or polymer, coating a layer of SU8 photoresist on a homogenizer, and baking on a hot plate at 85 degrees for 20 minutes; the coated photoresist The glued substrate is placed in a photolithography machine, the surface of the substrate is covered with a patterned mask, and exposed under ultraviolet light with an exposure intensity of 100~500mW / cm 2 , the exposure time is between 10 and 200s, and the pattern is transferred to SU8 glue to form a polymerized area and an unpolymerized area; the substrate coated with photoresist is placed on the aluminum block connected to the ground wire of the high-voltage power supply, and the tip of the syringe needle is ground level and connect the positive pole of the high voltage power supply. Use a syringe pump to push the syringe, so ...

Embodiment 2

[0035] By designing with different patterns and making mask plates, follow figure 1 The flow shown performs compartmentalization of electrospinning to prepare compartmentalized electrospinning with different patterns, such as image 3 shown.

Embodiment 3

[0037] Electrospinning arrays were prepared for three-dimensional cell culture by regionalized electrospinning. Using the method mentioned in Example 1, PS electrospinning arrays were prepared for three-dimensional cell culture. From Figure 4 It can be seen that the cells can grow for a long time on the regionalized electrospinning, and present a typical three-dimensional growth state. Therefore, multi-parameter and multi-indicator cell culture can be realized.

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Abstract

The invention provides a universal nanoscale electrospining regionalization method. The universal nanoscale electrospining regionalization method comprises that according to thermal melting properties of an unpolymerized SU8 photoresist, through exposure under ultraviolet exposure conditions, a SU8 photoresist coated on a glass support plate so that polymerization and non-polymerization zones are formed; the treated SU8 photoresist is arranged under an electrostatic spinning device so that an electrically spun layer is formed on the surface of the SU8 photoresist; the treated SU8 photoresist is arranged in an oven and then is heated so that the unpolymerized SU8 photoresist are melted into a liquid and the volume of the molten SU8 photoresist is increased and the molten SU8 photoresist wraps electrically spun threads; and the treated SU8 photoresist is cooled at a room temperature and is subjected to ultraviolet exposure so that the unpolymerized SU8 photoresist is polymerized and electrospining regionalization is realized. The universal nanoscale electrospining regionalization method has the advantages that nanoscale electrospining patterns obtained by the universal nanoscale electrospining regionalization method can provide an application platform having a potential for tissue engineering and regenerative medicine engineering; processes are simple; controllability is strong; and the universal nanoscale electrospining regionalization method can combine with novel technologies such as microfluidic easily.

Description

technical field [0001] The invention belongs to the fields of microfluidic chip technology, nano-electrospinning technology, polymer material science, and cytology application thereof, and specifically relates to a general-purpose nano-electrospinning regionalization method. Background technique [0002] Due to the high specific surface area, porous structure and surface chemical properties of various prepared materials, nano-electrospinning has shown broad application prospects in the field of biology. Its application fields mainly include the following aspects: 1. Electrospinning is used as the substrate for three-dimensional culture of cells to study the behavior of cells in a three-dimensional environment; 2. Electrospinning is used as a drug or gene carrier for cell drug screening or Research on exogenous gene transfection; 3. Electrospinning is used as a scaffold in vivo to promote the repair of damaged tissues; 4. Using electrospinning to reconstruct tissues and organ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00D01D5/00
Inventor 秦建华姜雷张旭
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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