Preparation method of tubular porous bacterial cellulose

A bacterial cellulose and tubular technology is applied in the field of preparation of tubular porous bacterial cellulose, which can solve the problems of poor suture performance, insufficient compliance, and intimal hyperplasia, and achieve the effect of improving production efficiency.

Inactive Publication Date: 2013-03-20
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tubular bacterial cellulose prepared only by a single-layer oxygen-permeable material has an asymmetric structure with a dense inner cavity and a loose outer wall.
The loose outer wall makes the m

Method used

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Examples

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Effect test

preparation example Construction

[0054] The invention relates to a preparation method of tubular bacterial cellulose. Bacterial cellulose is cultivated by nesting double-layer oxygen-permeable materials to form a hollow oxygen-permeable mold. On the basis of the existing disclosed technology, the inner tube and the outer tube of the hollow oxygen permeable mold can shrink radially / diastolic periodic alternation. Since the magnitude of radial deformation is very small, no more than 5%, the cultivation process is a "quasi-static" process. The invention doubles the "effective area" inside the bacterial cellulose, shortens the cultivation period of the tubular bacterial cellulose, and at the same time makes the outer wall of the tubular bacterial cellulose denser, improves the sutureability of the material, and reduces the number of wounds at the suture after being implanted into the human body. Probability of intimal hyperplasia and thrombosis.

[0055] A method for preparing tubular porous bacterial cellulo...

Embodiment 1

[0063] 1) Preparation of fermentation medium;

[0064] Components of the fermentation medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, and amount of water

[0065] The pH of the fermentation broth is 4.0;

[0066] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0067] 2) Bacteria expansion;

[0068] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 5 pieces / ml.

[0069] 3) Static cultivation;

[0070] After the expanded bacterial liquid is evenly mixed with the prepared fermentation culture liquid, it is poured into the area between the outer wall of the inner tube and the inner wall of the outer ...

Embodiment 2

[0080] 1) Preparation of fermentation medium;

[0081] Components of the fermentation medium, in mass percent, in wt%: 5 glucose, fructose, sucrose or mannitol, 0.5 peptone, 0.5 yeast extract, 0.1 citric acid, 0.2 disodium hydrogen phosphate, 0.1 potassium dihydrogen phosphate, and The amount is water; the pH of the fermentation broth is 6.0;

[0082] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0083] 2) Bacteria expansion;

[0084] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 pieces / ml.

[0085] 3) Static cultivation;

[0086] After the expanded bacterial liquid is evenly mixed with the prepared fermentation culture liquid, it is poured into the area between the outer wall of the inner tube of the hollow oxygen-permeable mold and th...

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PUM

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Abstract

The invention relates to a preparation method of tubular porous bacterial cellulose. According to the preparation method, double layers of oxygen-permeating materials are nested to form a hollow oxygen-permeating mold for culturing bacterial cellulose, an inner pipe and an outer pipe of the mold can shrink/relax along the radial direction in a periodical alternation manner by accurately adjusting the pressure and the oxygen volume concentration of the internal pressure gas of the inner pipe and the external gas of the outer pipe of the hollow oxygen-permeating mold; and simultaneously sustained release microspheres which can release oxygen or a sugar source slowly are added into a culture solution so as to prepare the tubular porous bacterial cellulose. According to the preparation method, the effective area in the bacterial cellulose is expanded by more than one time, the culture period is shortened, the outer wall of the tubular bacterial cellulose is compact, the suturing performance of materials is improved, the probability of intimal hyperplasia and thrombus at the suturing position after the tubular porous bacterial cellulose is planted into a human body is lowered, and a formed porous structure is beneficial to the entering of cells and nutrient substances into the materials; and the material preparation period is short, the preparation process is environment-friendly, simple and fast, and the preparation cost is low.

Description

technical field [0001] The invention relates to a method for preparing tubular porous bacterial cellulose, in particular to a method for preparing tubular porous bacterial cellulose with a porous structure. Background technique [0002] Bacterial cellulose refers to microorganisms that can biosynthesize cellulose under different conditions, including: Acetobacter xylinum, Acetobacter, Acetobacter, Acetobacter pasteurian, Glucobacterium, Agrobacterium, Rhizobium, Sarcina, A collective term for cellulose synthesized by one or more microorganisms in Pseudomonas cepacia, Pseudomonas coco or Campylobacter jejuni. Acetobacter xylinum in the genus Acetobacter is widely used as a template microorganism for basic and applied research on bacterial cellulose because of its highest cellulose production efficiency. [0003] The preparation methods of bacterial cellulose are mainly static culture and dynamic culture. Due to the oxygen-dependent survival properties of the Gram-negative p...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/01C12R1/02C12R1/38C12R1/41
Inventor 杨敬轩李喆郑羿王利群王华平陈仕艳张云
Owner DONGHUA UNIV
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