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Method for extracting DEHP (di(2-ethylhexyl)phthalate) from fermentation product of ocean paenibacillus polymyxa L1-9 and application

A technology of Paenibacillus polymyxa and fermentation products, which is applied in the field of extracting active ingredients and can solve problems such as no research reports on Paenibacillus polymyxa

Inactive Publication Date: 2013-04-03
HUAIHAI INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The Paenibacillus polymyxa that has been studied is mainly isolated from the soil, and a few are plant endophytes, all of which are in terrestrial environments. There are no research reports on Paenibacillus polymyxa from the ocean

Method used

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  • Method for extracting DEHP (di(2-ethylhexyl)phthalate) from fermentation product of ocean paenibacillus polymyxa L1-9 and application
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  • Method for extracting DEHP (di(2-ethylhexyl)phthalate) from fermentation product of ocean paenibacillus polymyxa L1-9 and application

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Embodiment 1

[0072] Embodiment 1, a method for extracting DEHP from a marine Paenibacillus polymyxa L1-9 fermentation product, the steps are as follows:

[0073] (1) L 1The preparation of -9 bacterial strain fermented liquid: use fermentor to L 1 -9 strains were fermented in batches, and the fermentation conditions were: the bacterial age of the seed liquid was 24h, and the concentration was 10 9 cells / mL, inoculum size 8%, temperature 28°C, pH 6.3, initial stirring speed 250r / min, ventilation rate 3L / min, fermentation time 36h; 1 -9 strain fermentation broth; L 1 After centrifugation at 10,000r / min, the fermentation broth of -9 strains was tested for antibacterial activity against Fusarium graminearum with 50 μl / well, and the L 1 -9 strain fermentation broth was initially extracted;

[0074] (2) Preliminary extraction of antibacterial active substances: L 1 The fermented liquid of strain -9 was extracted twice with an equal volume of ethyl acetate, and after standing for 12 hours, th...

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Abstract

The invention discloses a method for extracting DEHP (di(2-ethylhexyl)phthalate) from a fermentation product of ocean paenibacillus polymyxa L1-9. The method takes fusarium graminearum as an indicator and performs separation and purification of the antibacterial-activity components generated by the strain of paenibacillus polymyxa L1-9 by means of column chromatography on silica gel, SephadexLH-20 column chromatography, HPLC (high performance liquid chromatography) and the like to obtain an antifungal compound B2; and according to the structural identification by the spectrum technologies such as ESI-MS, <1>HNMR, <13>CNMR and the like, B2 is DEHP. The method disclosed by the invention separates DEHP from the metabolic product of paenibacillus polymyxa; and experiments prove that DEHP has an obvious inhibition effect on fusarium graminearum. The bacteria have the advantages that the growth period is short, large-scale culture is easy to implement in a laboratory, the fermentation product is easier to purity and the like, and are favorable for large-scale fermentation to realize industrial production.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from marine bacteria fermentation products, especially a marine Paenibacillus polymyxa L 1 -9 A method for extracting DEHP from a fermentation product, the present invention also relates to the use of the aforementioned DEHP. Background technique [0002] Paenibacillus polymyxa ( Paenibacillus polymyxa ) as important plant disease biocontrol bacteria and plant rhizosphere growth-promoting bacteria have been widely used in the agricultural field. Paenibacillus polymyxa can produce a variety of antibacterial substances such as peptides, proteins, nucleosides, pyrazines and phenols. For example, the protein produced by Paenibacillus polymyxa VLB16 can inhibit the rice blast fungus ( Magnaporthe oryzae ) and Rhizoctonia solani ( Rhizoctonia solani ) growth, its molecular weight is 37KDa, and it can withstand the high temperature of 121 ℃. Beatty et al. isolated a cluster of antibacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C07C69/80C07C67/56A01N37/10A01P3/00C12R1/01
Inventor 暴增海马桂珍吴少杰王淑芳付泓润葛平华
Owner HUAIHAI INST OF TECH
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