Method for producing ethanol by using wood fiber raw material
A technology for lignocellulosic raw materials and ethanol, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low yield of total sugar, etc., and achieves the improvement of ethanol concentration, utilization rate of total sugar, and residence time. short effect
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Embodiment 1
[0024] In the first stage of the three-stage screw extruder, 500 grams of corn stover was pulverized, and then continuously impregnated with 1.5% dilute sulfuric acid at 60°C for a residence time of 7 minutes, extruded, dehydrated and preliminarily expanded. In the second stage of the three-stage screw extruder, the dehydrated corn stover was maintained at 160° C. and 0.7 MPa for 20 minutes. In the third stage of the three-stage screw extruder, the cooked material is extruded, steamed and exploded by the screw, and instantly decompressed and puffed to obtain a porous, fluffy and wet material. After adding water to the above-mentioned porous fluffy wet material (the dry matter concentration ratio is 17%), the concentration of penicillin added is 3×10 -4 g / L, the temperature is controlled at 55°C, and under the condition of pH=5.0, cellulase is added in an amount of 0.14 g / g cellulose, and enzymatic hydrolysis obtains an enzymatic hydrolysis solution containing pentose, hexose a...
Embodiment 2
[0027] In the first stage of the three-stage screw extruder, 500 grams of wheat straw was pulverized, and then continuously impregnated with 1.5% dilute sulfuric acid at 60°C. After a residence time of 9 minutes, it was extruded, dehydrated and preliminarily expanded. In the second stage of the three-stage screw extruder, the dehydrated wheat straw was maintained at 170° C. and 0.8 MPa for 18 minutes. In the third stage of the three-stage screw extruder, the cooked material is extruded, steamed and exploded by the screw, and instantly decompressed and puffed to obtain a porous, fluffy and wet material. After the above-mentioned porous fluffy wet material is added with water (the dry matter concentration ratio is 26%), the concentration of penicillin added is 3×10 -4 g / L, the temperature is controlled at 55°C, and under the condition of pH=4.5, cellulase is added in an amount of 0.15 g / g cellulose, and enzymatic hydrolysis obtains an enzymatic hydrolysis solution containing pen...
Embodiment 3
[0030] In the first stage of the three-stage screw extruder, 500 grams of rice straw was pulverized, then continuously impregnated with 1.5% dilute sulfuric acid at 60°C, and after a residence time of 8 minutes, extruded, dehydrated and preliminarily expanded. The dehydrated rice straw in the second stage of the three-stage screw extruder was maintained at 170° C. and 0.8 MPa for 25 minutes. In the third stage of the three-stage screw extruder, the cooked material is extruded, steamed and exploded by the screw, and instantly decompressed and puffed to obtain a porous, fluffy and wet material. After the above-mentioned porous fluffy wet material is added with water (the dry matter concentration ratio is 25%), the concentration of penicillin added is 3×10 -4 g / L, the temperature is controlled at 55°C, and under the condition of pH=4.5, cellulase is added in an amount of 0.14 g / g cellulose, and enzymatic hydrolysis obtains an enzymatic hydrolysis solution containing pentose, hexo...
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