Method for preparing salvianolic acid A

A salvianolic acid and salvianolic acid-containing technology, applied in the field of preparation of salvianolic acid A, can solve the problems of high cost, serious organic residues, difficulty in storage and use, etc.

Active Publication Date: 2013-04-17
JIANGXI KEWEI COLLABORATIVE INNOVATION PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost is extremely high, the organic residue is serious, and it is extremely difficult to store and use
[0...

Method used

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  • Method for preparing salvianolic acid A
  • Method for preparing salvianolic acid A
  • Method for preparing salvianolic acid A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Take Danshen medicinal material, crush it into 6-mesh granules, add 7 times the amount of 92°C water each time, warm soak and extract 3 times, and stir at a speed of 25 rpm, and warm soak and extract for 3 hours each time; the extract is concentrated under reduced pressure to relative Density 1.20 (60°C), add ethanol to make the alcohol content at 70%, let it stand, filter, the filtrate is decompressed to recover ethanol and concentrate until it has no alcohol smell; add water to dilute to contain salvianolic acid B20mg per 1ml, use 10% for aqueous solution Adjust the pH to 4.0 with sodium hydroxide, add 0.5% ZnCl 2 As a catalyst, heat and transform at 120°C for 4 hours, adjust the pH value of the transformation liquid to 2.5 with 20% phosphoric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 3 mg of salvianolic acid A per 1 ml, and perform HPD-100 macroporous resin column chromatography For separation, the ratio of the loading amount of ...

Embodiment 2

[0058] Take Salvia miltiorrhiza, cut into decoction pieces, add 8 times the amount each time, soak in water at 85°C for 3 times, stir at a speed of 20 rpm, and extract with warm soaking for 2.5 hours each time; the extract is concentrated under reduced pressure to a relative density of 1.20 (60 ℃), add ethanol to make the alcohol content at 75%, let it stand, filter, and the filtrate reclaims ethanol under reduced pressure and concentrates to no alcohol smell; add water to dilute to contain salvianolic acid B15mg per 1ml, adjust the aqueous solution with 10% potassium hydroxide pH to 4.0, add 0.6% ZnCl 2 As a catalyst, heat conversion at 120°C for 3.5 hours, adjust the pH value of the conversion liquid to 2.5 with 15% hydrochloric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 5 mg of salvianolic acid A per 1 ml, pass through HPD-100 macroporous resin column layer Analysis and separation, the ratio of the loading amount of salvianolic acid A to...

Embodiment 3

[0061] Take the Danshen medicinal material, crush it into particles with a diameter of about 2mm, add 9 times the amount each time, soak in water at 85°C for 2 times, and stir at a speed of 30 rpm, and extract with warm soaking for 3.5 hours each time; the extract is concentrated under reduced pressure to a relative density 1.15 (60°C), add ethanol to make the alcohol content at 75%, let it stand, filter, the filtrate is decompressed to recover ethanol and concentrate until it has no alcohol smell; add water to dilute to 18mg per 1ml of salvianolic acid B, and add 10% carbonic acid to the aqueous solution Adjust pH to 4.2 with sodium, add 0.6% ZnCl 2 As a catalyst, heat conversion at 123°C for 4.5 hours, adjust the pH value of the conversion liquid to 2.8 with 15% nitric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 6 mg of salvianolic acid A per 1 ml, and pass through HPD-100 macroporous resin column layer Analysis and separation, the ratio o...

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PUM

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Abstract

The invention relates to a method for preparing salvianolic acid A. According to the method, salvia miltiorrhiza extract is prepared from salvia miltiorrhiza medicinal materials and is subjected to microwave vacuum drying after the salvia miltiorrhiza extract is subjected to catalytic conversion and the steps of separation, elution and the like. The method for preparing the salvianolic acid A, provided by the invention, is very applicable to industrial application, lower in production cost and novel.

Description

technical field [0001] The invention relates to a preparation method of salvianolic acid A. technical background [0002] Salvia miltiorrhiza preparation is the basic treatment drug for cardiovascular and cerebrovascular diseases in my country. Because of its definite curative effect, Danshen has become one of the traditional Chinese medicines with the largest consumption, the highest sales, the most preparation factories and the most complete clinical dosage forms in China. There are two main types of effective chemical components in Danshen: fat-soluble tanshinone compounds and water-soluble phenolic compounds. Studies have shown that salvianolic acids have significant activities in anti-liver damage, anti-atherosclerosis and apoptosis, and improvement of memory dysfunction. Among them, salvianolic acid A (Salvianolic acid A) has the strongest antioxidant activity, and the structure of salvianolic acid A is as follows: [0003] [0004] However, the natural content of...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/32
Inventor 吕武清杨小玲欧阳婷蔡元魁钟阳桂崔刚
Owner JIANGXI KEWEI COLLABORATIVE INNOVATION PHARM CO LTD
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