Electrochemical luminescence immune sensor built by mesoporous material loading quantum dots and coated by nanogold, and detection method of HIV (human immunodeficiency virus)

A luminescence immunity, sensor technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, scientific instruments, etc., can solve the problems of low background signal of excitation spectrum, narrow emission spectrum, etc., to achieve wide application prospects, High sensitivity, simple detection effect

Inactive Publication Date: 2013-04-17
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a new type of electrochemiluminescent source material, quantum dots have the advantages of controllable particle size, narrow emission spectrum, wide excitation spectrum and low background signal, and are widely used in the construction of biological immunosensors. There are a large number of literatures reporting the application of quantum dots in bioimaging and bioimmune analysis, but the preparation of high-efficiency and signal-based immunosensors based on the huge specific surface area and pore volume of mesoporous materials has not yet been reported.

Method used

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  • Electrochemical luminescence immune sensor built by mesoporous material loading quantum dots and coated by nanogold, and detection method of HIV (human immunodeficiency virus)
  • Electrochemical luminescence immune sensor built by mesoporous material loading quantum dots and coated by nanogold, and detection method of HIV (human immunodeficiency virus)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Preparation of mesoporous silica loaded with quantum dots and coated with gold nanoparticles

[0025] Quantum dots used in the present invention, mesoporous silicon oxide, and nano-gold are respectively synthesized according to the method reported in the literature, and the specific method is referred to in the literature (Talanta, 80, 1737-1743 (2010), Langmuir, 27, 6099-6106 (2011), Anal .Chem., 77,3324-3329(2005).The specific preparation scheme of the mesoporous silica loaded with quantum dots and coated with nano gold is as follows:

[0026] (1) Disperse 50mg of mesoporous silica in 10mL of anhydrous toluene, add the same volume of water-soluble CdTe quantum dots and stir evenly at 40°C for 24 hours, centrifuge, wash with distilled water and absolute ethanol at least three times each, vacuum Dry to obtain mercapto-modified mesoporous silica.

[0027] (2) Disperse 20 mg of mercapto-modified mesoporous silica in 10 mL of tetrahydrofuran, add the same volum...

Embodiment 2

[0029] Example 2: Construction of Electrochemiluminescence Immunosensor

[0030] The present invention constructs an electrochemiluminescent immunosensor by using the above-mentioned mesoporous silicon oxide loaded with quantum dots and coated with nano-gold on the surface together with magnetic beads, and the specific embodiments are as follows:

[0031] (1) Preparation of magnetic capture probe: 10mg mL -1 Ultrasonic dispersion of magnetic beads in PBS buffer at pH 7.4, pipette 1mL, 50μg mL -1 Goat anti-human anti-antibody anti-IgG was added, mixed and stirred at 4°C for 6 hours, and the active sites of other unbound antibodies were blocked with 1 mL of 1% bovine serum albumin (BSA), and the anti-anti-body that was not fixed by magnetic beads was washed by magnetic separation. -IgG, after washing, redisperse in 1mL PBS solution of pH7.4 to obtain a magnetic capture probe (NMP / anti-IgG); put the magnetic capture probe (NMP / anti-IgG) prepared above into the solution Add a ce...

Embodiment 3

[0034] Example 3 Electrochemiluminescence response of several different modified electrodes

[0035] Using different materials to modify the electrode, compare its electrochemiluminescent response, the results are as follows figure 1 shown. bare electrode ( figure 1 , curve a) contains 0.1mol / L PBS (pH=7.4) and 0.1mol / L K in 0.1mol / L KCl 2 S 2 o 8 Electrochemiluminescence behavior in solution, the electrochemiluminescence response signal is very weak; when the electrode surface modified NMP / anti-IgG ( figure 1 , after curve b), its electrochemiluminescent signal is also very weak, this is because the electrode surface is covered with a layer of insulating protein layer, and there is no electrochemiluminescent source material, so its electrochemiluminescent signal is almost non-existent; the same Principle, when the electrode surface is modified with NMP / anti-IgG / Ab( figure 1 , after curve c), the electrochemiluminescence signal also remains unchanged; when the electrode ...

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Abstract

The invention relates to the field of analysis tests, and discloses an electrochemical luminescence immune sensor built on the basis of a mesoporous material which loads quantum dots and is coated by nanogold, and a sandwich immunoassay method for detecting an HIV (human immunodeficiency virus) antibody. The electrochemical luminescence immune sensor takes the mesoporous material which loads the quantum dots and is coated by the nanogold as a signal label; and immune complex is formed by a biological immune method and decorated to the surface of an electrode, so as to form the electrochemical luminescence immune sensor. By adopting the mesoporous material which loads the quantum dots and is coated by the nanogold, a lot of quantum dots are loaded on the basis of the mesoporous material; a substance of a signal source is increased; the coated nanogold can be used for a method signal, and also can increase the biocompatibility; and the detection method is simple in sample pretreatment, and can adapt to high-sensitivity detection and analysis of the content of the HIV antibody in a serum sample.

Description

technical field [0001] The present invention relates to the detection and analysis of AIDS virus (HIV) antibody, and its basic method is based on a mesoporous material loaded with quantum dots and coated with nano-gold as a signal label to construct an HIV immune sensor, and through electrochemiluminescence analysis technology, Establish a highly sensitive and selective quantitative detection and analysis method for HIV antibodies. Background technique [0002] Acquired Immunodeficiency Syndrome, also known as AIDS (AIDS), is caused by infection with the Human Immunodeficiency Virus (HIV). HIV is very harmful and has a wide range of transmission. Currently there is no specific drug to treat AIDS. Therefore, early diagnosis and monitoring of potential HIV infections and AIDS patients are strengthened to achieve the purpose of controlling the epidemic of AIDS. An important topic and goal of treatment and prevention work. At present, the clinical diagnosis of HIV infection ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/571
Inventor 干宁周汉坤李天华曾少林周靖熊萍杜晓雯
Owner NINGBO UNIV
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