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Immobilized algae cell bioelectrode, and usage and test method thereof

A bio-electrode, green algae technology, applied in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of unreported, reduced electron transfer efficiency, etc., to achieve convenient operation, improve electron transfer efficiency, and reduce recycle The effect of combined risk

Inactive Publication Date: 2013-04-17
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Looking at these similar studies, the key issue is how to realize the efficient transfer of electrons between the biochemical reaction center and the electrode of the battery. The enhancement of mediator recombination with oxygen will greatly reduce the electron transfer efficiency
Immobilizing microalgae cells on the electrode material can effectively solve this problem. Although there are a lot of reports on the immobilization of algae cells in the existing research, the preparation of green algae on the electrode material by fixing algae cells with silica sol-gel method can effectively solve this problem. The use of bioelectrodes combined with mediators in the study of light-induced electron transfer has not been reported at home and abroad

Method used

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  • Immobilized algae cell bioelectrode, and usage and test method thereof
  • Immobilized algae cell bioelectrode, and usage and test method thereof
  • Immobilized algae cell bioelectrode, and usage and test method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The response of the sub-heart-type Tetraphyllum bioelectrode to light

[0036] (1) Preparation of silica sol: slowly drip 10mL tetraethoxysilane into a stirring system of 60mL water and 30mL 0.01M HCl. After reacting for 50h, adjust the pH to 7.50 with 1M NaOH solution, and then react for 48h. The pH is 7.81, and the prepared silica sol is ready for use.

[0037] (2) Take the late growth logarithm, the concentration is about 3.50×10 6 57 mL of subcardiotype Tetraphyllum cells / mL was concentrated by centrifugation at 2000 r / min for 1 min, and the supernatant was discarded to obtain the algal cells. The algae cells obtained by centrifugation were resuspended in 2 mL of silica sol, and the volume of the algae-silica sol mixture was 50 μL in a pipette, and dried in the air to form a green algae bioelectrode.

[0038] (3) In the three-electrode system, the working electrode is a green algae bioelectrode, the counter electrode is a platinum electrode or a titanium electrode, the re...

Embodiment 2

[0041] Light response under different light intensities

[0042] (1) Preparation of silica sol: slowly drop 10mL tetraethoxysilane into a stirring system of 60mL water and 30mL 0.01M HCl. After reacting for 48h, adjust the pH to 7.49 with 1M NaOH solution, and then react for 4 days. The pH is 8.0, and the prepared silica sol is ready for use.

[0043] (2) Take the concentration of about 2.64×10 6 The cells / mL of the subcardiotype Tetraphyllum was 38 mL, the Fv / Fm of the algal cells was 0.754, and the yield was 0.606. After centrifugation at 2000 r / min for 1 min, the supernatant was discarded to obtain the algal cells. The algae cells obtained by centrifugation were resuspended in 1 mL of silica sol, and the volume of the algae-silica sol mixture was 50 μL in a pipette and dried in the air to form a green algae bioelectrode.

[0044] (3) In the three-electrode system, the working electrode is a green algae bioelectrode, the counter electrode is a platinum electrode or a titanium elec...

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Abstract

The invention relates to an immobilized algae cell bioelectrode and a usage method thereof. The bioelectrode is prepared by immobilizing algae cells on a planar porous electrode material by using a sol-gel method. The prepared bioelectrode is used as a work electrode and constitutes a three-electrode system with a counter electrode and a reference electrode, an electronic mediator is added in electrolyte and an external voltage is applied so as to perform light response test on the electrode. Electron transfer between an algae photosynthetic system and an electrochemical system can be realized by using the prepared bioelectrode, a light current of 94.2 microamperes which is 25 times that of an algae cell suspension system can be obtained. No technology used for light current researches of preparing the bioelectrode by using silica gel immobilized microalgaes is reported internationally. The bioelectrode can realize high-efficiency couple between the biological photosynthetic system and the electrochemical system, characterizes a photoinduced electron transfer process of a biosystem, realizes direct photoelectric or light energy-hydrogen energy transfer, and has the advantage of high efficiency with low cost, and is simple and practicable.

Description

Technical field [0001] The invention relates to a bioelectrode for immobilizing green algae cells and a method of use thereof, that is, using a silica sol-gel method to fix the green algae cells on a planar porous electrode material to construct a green algae bioelectrode. An electron mediator is added to the electrolyte to realize the electron transfer between the photoreaction center of the green algae cell and the electrode, and the electrode is tested with a three-electrode system and an external voltage. Background technique [0002] Hydrogen production from green algae is currently a research hotspot in the field of hydrogen production. Chlorella can decompose water into oxygen, protons and electrons in the process of photosynthesis II. The electrons pass through the electron transport chain of photosynthetic system I and ferredoxin to hydrogenase, which reduces protons to hydrogen. In the process of hydrogen production by green algae, the sources of hydrogen-producing ele...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 陈兆安吕艳霞陆洪斌周建男
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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