Ready-to-use adjuvant of livestock vaccines, preparation and applications thereof

A vaccine and adjuvant technology, applied in the field of compound adjuvant preparation, can solve the problems of inability to meet the needs of epidemic disease prevention and control in aquaculture industry, inability to continuously stimulate the immune response of the body, and uncommon application of vaccines, so as to prolong the protection period and improve the retention. and presentation ability, the effect of simple process

Active Publication Date: 2013-05-01
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the vaccine application of this dosage form is not common, and it is only commercialized in the application of human vaccines. For example, the MF59 adjuvant is used in the production of influenza vaccines, and has not been widely used in animal vaccines.
The reason is that the oil-in-water (O / W) formulation vaccine has weak immunogenicity and only induces a weak immune response in animals, which cannot meet the needs of epidemic prevention and control in the aquaculture industry.
At the same time, the existing oil-in-water (O / W) dosage form vaccine has no slow-control and slow-release effect, and one immunization cannot continuously stimulate the body's immune response, and the duration of immunity is very short

Method used

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  • Ready-to-use adjuvant of livestock vaccines, preparation and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Adjuvant preparation

[0032] Recipe 1:

[0033] White oil 10 g,

[0034] Tween 80 0.5g,

[0035] Sibon 85 0.5g,

[0036] Chitosan 2.0g,

[0037] Carbomer 971P 0.1g,

[0038] Water 83.9g.

[0039] Recipe 2:

[0040] Squalane 10 g,

[0041] Pluronic 121 0.5g,

[0042] Sibon 80 0.5g,

[0043] Carbomer 971P 3.0g,

[0044] Water 83.0g.

[0045] Recipe 3:

[0046] Squalane 10g, Squalene 10g

[0047] Pluronic 121 5g, Tween 80 0.3g,

[0048] Siben 80 5g, Siben 85 0.3g,

[0049] Hydroxymethyl Cellulose 1.0g, Dimethyl Dioctadecyl Ammonium Bromide 1.0g,

[0050] Water 67.4g.

[0051] Recipe 4:

[0052] Squalane 15g, white oil 15g,

[0053] Pluronic 121 0.2g, Tween 80 8.8g,

[0054] Siben 80 0.2g, Siben 85 8.8g,

[0055] Hydroxymethyl Cellulose 2.0g, Carbomer 971P 3.0g,

[0056] Water 47.0g.

[0057] Recipe 5:

[0058] Squalane 5 g,

[0059] Pluronic 121 0.1g,

[0060] Siben 80 0.1g,

[0061] Carbomer 971P 1.0g, Dimethyl Dioctadecyl Ammonium Br...

Embodiment 2

[0071] Example 2 Preparation and properties of oil-in-water vaccine against porcine foot-and-mouth disease

[0072] (1) Preparation of ready-to-use adjuvants

[0073] The ready-to-use adjuvants 1-6 were prepared according to the method in Example 1. ISA206 adjuvant was purchased from SEPPIC Company.

[0074] (2) Prepare porcine foot-and-mouth disease inactivated virus liquid

[0075]Inactivated porcine foot-and-mouth disease virus liquid (type O, II) was provided by Jinyu Baoling Biological Pharmaceutical Co., Ltd., and the virus content in every 0.2 ml of virus liquid before inactivation was 10 7.0 LD 50 / 0.2ml. The porcine foot-and-mouth disease inactivated virus liquid is prepared in accordance with the relevant requirements of "Compilation of Quality Standards for Veterinary Biological Quality" (2006-2008). Pig foot-and-mouth disease inactivated virus liquid (type O, II) is referred to as pig foot-and-mouth disease inactivated virus liquid.

[0076] (3) Preparati...

Embodiment 3

[0089] Example 3 Application of oil-in-water porcine circovirus vaccine

[0090] (1) Preparation of porcine circovirus type 2 inactivated virus solution

[0091] Porcine circovirus type 2 inactivated virus liquid (DBN-SX07 strain) was provided by Chengdu Tianbang Biotechnology Co., Ltd., and the virus content per ml of virus liquid before inactivation was 10 5.5 TCID 50 . The inactivated virus liquid complies with the relevant requirements of the "Compilation of Quality Standards for Veterinary Biological Quality" (2006-2008).

[0092] (2) Preparation of oil-in-water porcine circovirus vaccine

[0093] The ready-to-use adjuvants 1-6 were mixed with the porcine circovirus type 2 inactivated virus solution respectively, and the oil-in-water porcine circovirus vaccines 1-6 were prepared evenly by hand shaking. The mass ratio of the ready-to-use adjuvant 1 in the oil-in-water porcine circovirus vaccine 1 to the porcine circovirus type 2 inactivated virus liquid is 1:0.5. In...

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Abstract

The invention provides a ready-to-use adjuvant of livestock vaccines, preparation and applications thereof, belonging to the field of the adjuvant of livestock vaccines. The ready-to-use adjuvant is prepared from the following substances: 5-30 percent of oil, 0.1-10 percent of hydrophilic surfactant, 0.1-10 percent of oleophilic surfactant, 0.1-10 percent of polymeric micelle substance and 40-90 percent of water. The invention also provides a preparation method of the ready-to-use adjuvant, and the method comprises the following steps: respectively weighing the substances, mixing and then emulsifying, and degerming to obtain the ready-to-use adjuvant of the livestock vaccines. The invention also provides an oil-in-water type vaccine with the ready-to-use adjuvant. The ready-to-use adjuvant is an oil-in-water type adjuvant and has the advantages of simple ingredients, stable dosage form, convenience in use and easiness in injection. The preparation method of the ready-to-use adjuvant has a simple process and is low in cost, and the obtained dosage form is stable. The oil-in-water type vaccine has the advantages of long stable period, easiness in storage, good immune effect, protection period prolonging, easiness in injection and small side reaction.

Description

technical field [0001] The invention relates to the field of veterinary vaccine adjuvants, in particular to a method for preparing a compound adjuvant and a method for preparing a vaccine containing the compound adjuvant. Background technique [0002] Oil-based adjuvant vaccines are known to be effective in strengthening immunity. They are one of the most popular types of vaccines in the domestic and foreign vaccine market. There are three types of oil-containing adjuvant vaccines: water-in-oil (W / O), water-in-oil Oil (O / W) type and water-in-oil-in-water (W / O / W) type. [0003] The water-in-oil (W / O) type vaccine is the most commonly used. The significant advantage of this dosage form is that it can obtain a high level of long-lasting immune enhancement effect; the disadvantage is that it is thicker and difficult to inject. At the same time, due to the relatively high content of white oil in the vaccine , often leading to severe inoculation side effects, formation of sterile...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K9/107A61P37/04
Inventor 张金秋卢宇邓碧华吕芳侯继波
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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