Application of silver and platinum nano-cluster in tumor targeted imaging

A tumor-targeted imaging, platinum nanotechnology, applied in the field of medical imaging, can solve the problem of no silver, platinum nanoclusters, etc., and achieve the effect of small particle size, favorable absorption, and strong targeting

Active Publication Date: 2013-05-08
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese patent CN102735752A discloses the application of gold nanoclusters in tumor-targeted in vivo imaging.

Method used

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  • Application of silver and platinum nano-cluster in tumor targeted imaging
  • Application of silver and platinum nano-cluster in tumor targeted imaging
  • Application of silver and platinum nano-cluster in tumor targeted imaging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Liver cancer cells (HepG2) were selected as the research object, and the experimental group divided the liver cancer cells (HepG2) in the logarithmic growth phase at a ratio of 1.6×10 5 The density of cells / well was inoculated in 6-well plates, and after 24 h of culture, sterilized and diluted with fresh sterile DMEM medium containing 1 μmol / L glutathione-silver ammonia solution was added.

[0027] In the control group, the liver cancer cells (HepG2) in the culture medium were divided into 1.6×10 5 Cells / well were seeded in 6-well plates and cultured for 24 h. After the incubation time was terminated, phosphate buffer solution (PBS, pH=7.4) was added to each well of the experimental group and the control group to wash 2-3 times. Then observe and detect with a confocal microscope. The experimental results of the experimental group are as follows: Figure 1a As shown, the aggregation of silver nanoparticles can be observed, and the experimental results of the control gr...

Embodiment 2

[0029] Construct nude mice subcutaneously inoculated breast cancer model, breast cancer cells were cultured for 24 hours, then added 0.1 μ mol / L glutathione silver ammonia solution that had been sterilized and diluted with fresh aseptic DMEM medium, in the cell culture incubator Silver nanoclusters were prepared by incubation for 24 hours. The solution containing silver nanoclusters was ultrasonically dispersed and injected into nude mice through tail vein injection. After 24 hours, live fluorescence imaging was performed on nude mice. Experiments have shown that silver nanoclusters can achieve targeted imaging of tumor sites. Experimental results such as figure 2 shown.

Embodiment 3

[0031] The orthotopic transplantation model of liver cancer in nude mice was constructed. After the liver cancer cells were cultured for 24 hours, 50 μmol / L cis-diaminodibromoplatin solution, which had been sterilized and diluted with fresh sterile DMEM medium, was added and incubated in a cell culture box for 48 hours. Platinum nanoclusters were prepared in 1 hour, and the solution containing platinum nanoclusters was ultrasonically dispersed and injected into nude mice by local subcutaneous injection. After 24 hours, nuclear magnetic imaging was performed on nude mice and the imaging results were qualitatively and quantitatively analyzed.

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Abstract

The invention discloses application of silver and platinum nano-cluster in tumor targeted imaging. The application comprises the following steps: establishing nude mice tumor model; locally injecting ultrasonically dispersed silver or platinum nano-cluster into the nude mice, or directly injecting 0.1-100mu mol/L silver- or platinum-containing solution; medically imaging the nude mice tumor part by using a medical imaging method and performing qualitative and quantitative analysis on the imaging result after injection within 24 hours. The prepared silver and platinum nano-cluster is small in particle size, high in permeability, high in fluorescence stability, high in sensitivity and high in targeting property, and the tumor can be accurately positioned; the silver and platinum nano-cluster is directly synthesized through organisms and has high biocompatibility; the silver and platinum nano-cluster is injected through ultrasonic dispersion, so that the nano particles are fine and uniform, and absorption is facilitated; and moreover, the application is convenient to operate and intuitive in result.

Description

technical field [0001] The invention relates to the application of silver and platinum nano-clusters as tumor imaging agents in tumor targeting imaging, and the invention is applicable to the field of medical imaging. Background technique [0002] In vivo bioimaging technology is a powerful means to detect molecular and cellular events in experimental animals. It is being more and more widely used in the field of medical and biological research. It has extremely high detection sensitivity and low experimental cost. However, the technology still has many limitations, especially the tissue autofluorescence and Poor tissue permeability under broken wave excitation light. [0003] In order to overcome these difficulties. Scientists have studied a series of inorganic luminescent nanomaterials. Some inorganic nanomaterials, such as silicon nanoparticles, are widely used in biological analysis and imaging due to their excellent properties, but there are still some problems, the m...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/06A61K49/04A61K49/22
CPCA61K49/04A61K49/22A61K49/00A61K49/06A61K49/0002A61K49/0065A61K49/0414A61K49/1818A61K49/225
Inventor 王雪梅高生平吴长宇李水红叶静
Owner SOUTHEAST UNIV
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