Bacterium for preventing and controlling banana wilt and application thereof

A technology for fusarium wilt and banana, which is applied to bacteria for preventing and treating banana fusarium wilt and its application field, can solve the problems of banana fusarium wilt resistance transgenic breeding has not yet started, and the yield and quality of resistant varieties are not ideal, and achieves remarkable control effect, The effect of promoting rapid development

Inactive Publication Date: 2013-05-08
海南业勤香蕉产业技术开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the resistant varieties bred are often unsatisfactory in terms of yield and quality (Huang Bingzhi, Tang Xiaolang, Xu Linbing, etc. Introduction test of banana fusarium wilt-resistant variety Kangku No. 5. Chinese Fruit Tree, 2009, 6: 17- 19)
So far, banana genetically modified breeding for Fusarium wilt resistance has not started

Method used

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  • Bacterium for preventing and controlling banana wilt and application thereof
  • Bacterium for preventing and controlling banana wilt and application thereof
  • Bacterium for preventing and controlling banana wilt and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1. Isolation and Identification of Bacillus sp. Bsp.6

[0066] 1. Isolation of Bacillus sp. Bsp.6

[0067] 1. Using the tissue isolation method to obtain the strains in the plant tissue

[0068] Collect 1-2g of roots (0.2-0.5cm in diameter) from a healthy field banana plant in Hainan, China, wash with tap water for 10-30min, and then use 70% ethanol and mass percentage in turn Disinfect with 3.25% sodium hypochlorite for 1 min and 3 min, then rinse with sterile water 3 times. Put the roots in a mortar, add sterile water and grind thoroughly. After standing still for 15min, suck the supernatant and carry out 10 -1 、10 -2 、10 -3 Gradient dilution. Take 100 μL of the diluted solution and spread it on the NA medium plate, and apply 100 μL of the third sterile water rinse solution for the control. After culturing at 28°C for 3-5 days, pick single colonies with complete morphology and obvious differences, and streak culture on NA medium plates to obtain differe...

Embodiment 2

[0099] Example 2, Detection of antibacterial activity of Bacillus sp. Bsp.6CGMCC No.6459

[0100] In this example, the inhibitory effect of Bacillus sp. Bsp.6CGMCC No.6459 obtained in Example 1 on Fusantan oxysporum f.sp.cubense (Fusantan oxysporum f.sp.cubense) race 1 and race 4 was carried out. detected. Meanwhile, Bacillus sp. BEB2 was used as the control. The groups are shown in Table 4.

[0101] Table 4 Bacillus sp. (Bacillus sp.) Bsp.6CGMCC No.6459 antibacterial activity test group

[0102] group

Antagonist

pathogenic bacteria

1

Bacillus sp. Bsp.6CGMCC No.6459

Fusarium oxysporum race 1

2

Bacillus sp. BEB2

Fusarium oxysporum race 1

3

-

Fusarium oxysporum race 1

4

Bacillus sp. Bsp.6CGMCC No.6459

Fusarium oxysporum race 4

5

Bacillus sp. BEB2

Fusarium oxysporum race 4

6

-

Fusarium oxysporum race 4

[0103] Note: "-" indicates that the corresponding strain is n...

Embodiment 3

[0106] Example 3. Detection of the Control Effect of Bacillus sp. Bsp.6CGMCC No.6459 on Banana Fusarium Wilt

[0107] In this example, Bacillus sp. Bsp.6CGMCC No.6459 obtained in Example 1 is caused by Fusantan oxysporum f.sp.cubense No. 1 race and / or No. 4 race The control effect of banana fusarium wilt was tested in pot experiments. At the same time, Bacillus sp. BEB2 was used as the control strain. The experimental groups and strain inoculation methods are shown in Table 5.

[0108] Table 5 Bacillus sp. (Bacillus sp.) Bsp.6CGMCC No.6459 on the control effect of banana fusarium wilt pot experiment grouping and inoculation methods

[0109] group

First inoculation (0d)

Second inoculation (30d)

1

-

Fusarium oxysporum race 1

2

-

Fusarium oxysporum race 4

3

Bacillus sp. Bsp.6CGMCC No.6459

Fusarium oxysporum race 1

4

Bacillus sp. Bsp.6CGMCC No.6459

Fusarium oxysporum race 4

5

Bacillus sp. BEB...

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Abstract

The invention discloses a bacterium for preventing and controlling banana wilt and application thereof. The bacterium disclosed by the invention concretely is Bacillus sp. Bsp.6; and the preservation number in the China General Microbiological Culture Collection Center is CGMCC No.6459. The test shows that the Bacillus sp. Bsp.6 CGMCC No.6459 disclosed by the invention can restrain 1# and 4# microspecies of Fusantan oxysporumf. sp. cubense) at the same time; the bacterial agent prepared by taking the Bacillus sp. Bsp.6 CGMCC No.6459 as an active ingredient can prevent and control the banana wilt; the field control effect achieves about 95%; and the Bacillus sp. Bsp.6 CGMCC No.6459 disclosed by the invention and the bacterial agent have significant control effects on the banana wilt. Therefore, rapid development of banana plantation is facilitated to a large extent.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bacterium for preventing and controlling banana wilt and its application. Background technique [0002] Fenban is a hybrid cultivar of bananas. It is loved by consumers because of its small fruit size, beautiful appearance, golden skin, thin white flesh, tender and sweet taste, fragrance, and rich nutrition. In the past ten years, the cultivation of pink bananas has developed rapidly. At present, the planting areas of bananas in Guangxi, Guangdong and Hainan are about 5,000, 500 and 200 hectares respectively. Due to the hazards of Fusarium wilt, the large-scale planting of pink banana is not only severely restricted, but also requires crop rotation. Banana wilt (also known as Panama disease) caused by Fusarium oxysporumf.sp.cubense (E.F.Simth) Snyder et Hansen] is a devastating soil-borne fungal disease, since 1910 Panama banana wilt So far, the outbreak has caused heavy losses ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G13/00C12R1/07
Inventor 付业勤王必尊龙自梅金志强
Owner 海南业勤香蕉产业技术开发有限公司
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