Gene chip, detection reagent and kit for detection of NLRP7 gene

Abstract

The invention discloses an SNP (single nucleotide polymorphism) parting chip, a detection reagent and a kit for the detection of NLRP 7 gene related to hydatid mole, which builds a gene chip detection system for screening NLRP7 gene polymorphism high risk groups related to hydatid mole. The gene chip comprises a solid phase carrier and an oligonucleotide probe synthetized on the carrier, the detection reagent comprises a gene chip and 18 pairs of PCR (polymerase chain reaction) primers for amplifying all SNPs in a sample, and the kit comprises the detection reagent, a negative check sample and a positive check sample. The chip, the detection reagent and the kit can be used for detecting all SNPs locus related to the NLRP7 gene in the clinical sample rapidly and accurately. The detection system has large flux and high sensitivity, has significance for clinical diagnosis of hydatid mole as well as early screening and early prevention of high risk groups, and can be widely used in effective clinical screening of the hydatid mole high risk groups.

Description

technical field [0001] The invention relates to a gene chip, its detection reagent and kit, in particular to a gene chip, detection reagent and kit for detecting NLRP7 gene. Background technique [0002] Hydatidiform Mole (HM), the most common gynecological disorder, is an abnormal human pregnancy characterized by villous interstitial edema, trophoblast hyperplasia and lack of embryonic development or abnormal embryonic development. Moles are most commonly sporadic rather than recurrent, occurring at a rate of 1 per 1000-1500 pregnancies in South America, but higher rates in Latin America, the Middle East, and the Far East. my country and East Asia are high-incidence areas of hydatidiform mole, and the incidence rate is 7-10 times that of North America and European countries. In China, Zhejiang Province is the highest, with 1 time per 120-700 pregnancies; the pregnancy outcome of hydatidiform mole is worrying. According to different groups, 1-6% of women who have had a mole ...

AI Technical Summary

Problems solved by technology

The mutation rate of NLRP7 gene in Pakistani RHM patients (11 cases) was 81%, while that of Indian RHM patients (13 cases) was 84%. Mutations of subsons and promoters, or large deletions, rearrangements and duplications sometimes cannot be found by conventional DNA sequencing methods; 2. Only 20% of Chinese patients with recurrent mole have homozygous mutations, which is significantly lower than 88% of RHM patients in Pakistan and 72% of RHM patients in India, the Chinese race has a lower homology rate, which may be related to the large country, large population, and more opportunities for population migration

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