Device for detecting biological chips based on resonance light scattering

Abstract

The invention discloses a device for detecting biological chips based on resonance light scattering, which comprises a light source driver, an optical fiber, an excitation light source, a coupling lens group, a biological chip objective table, a photoelectric sensor, an upper computer, a motion platform and a multi-axle motor drive, wherein the light source driver controls the excitation light source to generate excitation light; the excitation light is outputted to the coupling lens group through the optical fiber, subjected to beam shaping, and transmitted to the biological chip objective table, and the excitation light is transmitted from the side edge of a biological chip under the condition that an angle of incidence is greater than a critical angle; a sample after being excited by the excitation light produces resonance light scattering, and radiates resonance scattering light with a specific wavelength outwards; the resonance scattering light is transmitted to the inside of the photoelectric sensor; the upper computer receives converted electric signals and carries out acquisition and analysis on the converted electric signals through software of the upper computer, thereby obtaining scanning images; and the software of the upper computer controls the multi-axle motor drive to operate and drives the motion platform to move, in such a way, the biological chip objective table is driven to move, so that the whole point sample area of the biological chip is scanned. The device disclosed by the invention is low in background noises and high in detection sensitivity.

Description

technical field [0001] The invention relates to the technical field of biochip detection, in particular to a device for detecting biochips based on resonance light scattering. Background technique [0002] Microarray is a high-tech that has developed rapidly in the field of life sciences in recent years. It mainly refers to the microbial chemical system constructed on the surface of a solid chip with only a few square centimeters through micro-processing technology to realize DNA Rapid, accurate and high-throughput detection of biological samples such as proteins, cells, etc. The main principle is to use chemical or physical methods to immobilize a large number of probes on the surface of the support, and then according to the affinity between biomolecules, Such as the base pairing of nucleic acid molecules, the combination of antigen and antibody, etc., and then detect and analyze the reaction signal, the relevant information of the sample can be obtained. Because this tec...

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Problems solved by technology

[0004] In order to solve the problems of excessive background noise and low sensitivity in detecting biochips in existing biochip scanners, the present invention provides a device for detecting biochips based on resonance light scattering

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