Light organism reaction device and application

A technology of photobioreaction and reactor, which is applied in photobioreactor, biochemical cleaning device, enzymology/microbiology device, etc., to achieve the effect of high biological cell density, simple operation and low shear force

Inactive Publication Date: 2013-06-05
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Aiming at the problems in the prior art, the object of the present invention is to provide a new type of photobiological reaction device, which integrates the advantages of the existing closed

Method used

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  • Light organism reaction device and application
  • Light organism reaction device and application

Examples

Experimental program
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Example Embodiment

[0036] Example 1

[0037] Use as figure 1 In the photobioreactor shown, the spherical diameter of the turbulent multistage spherical reactor is 50mm, the effective volume of the whole system is 2 liters, the gas distributor diameter is 10mm glass sand sintered porous plate, and the hole diameter is 80 microns. The entire system is made of hard borosilicate glass. 2-4 30W fluorescent tubes are evenly distributed around the reactor. Cultivate Isochrysis galbana, control the constant temperature water bath at 25-30℃, and pass in 3wt% CO 2 Air mixed gas, gas flow rate is 200ml / min, inoculation density is 0.15g / L dry weight, and the final density of algae cells reaches 2.5g / L after 5 days of cultivation.

Example Embodiment

[0038] Example 2

[0039] Adopt and figure 1 The device shown has a photobioreactor with the same configuration, in which the spherical diameter of the turbulent multistage spherical reactor is 35mm, the effective volume of the whole device is 1.1 liters, the gas distributor diameter is 6mm porous nozzle, and the hole diameter is 100 microns. The entire system is made of hard borosilicate glass. 2-4 30W fluorescent tubes are evenly distributed around the reactor. Cultivate Isochrysis galbana, control a constant temperature water bath at 25-30℃, and pass in 3wt% CO 2 Air mixed gas, gas flow rate is 200ml / min, inoculation density is 0.15g / l dry weight, after 3 days of cultivation, after the algae cell density reaches 1.5g / L solution in the sedimentation tank is released, add disinfected seawater to dilute and continue cultivation, After culturing for another 3 days, release the solution with a density of 2.0 g / L of algae cells in the sedimentation tank, and then add disinfected se...

Example Embodiment

[0040] Example 3

[0041] Use as attached figure 2 Shown is a photobiological reaction system composed of two sets of unit photobiological reaction devices connected in series. The turbulent multi-stage spherical reactor has a spherical diameter of 50mm, the effective volume of the entire system is 5.0 liters, and the gas distributor has a porous nozzle with a diameter of 6mm. The pore size is 100 microns. 2-4 pieces of 30W fluorescent tubes are evenly distributed around the reactor and the collected sunlight of a certain intensity is mixed into mixed light for irradiation at a certain distance, and the input contains 3wt% CO 2 Air mixed gas, gas flow rate is 300ml / min, seeding density is 0.13g / L dry weight, and the final density of algae cells reaches 2.8g / L after 7 days of cultivation.

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Abstract

The invention provides a light organism reaction device which can be used for culturing cell suspension of microalgae and other photosynthetic organisms. The light organism reaction device comprises a turbulence type multilevel spherical reactor, a reactor constant-temperature sleeving, a circulation diversion pipe, a circulation diversion pipe constant-temperature sleeving, a settling tank, a gap inlet, a gas distributor, a feeding opening, a light source, a constant-temperature waterbath, a gas flowmeter and the like. The light organism reaction device is a semicontinuous circulation gas lifting type light organism reactor, the cell suspension rises in the reactor in a turbulent mode in the flashing environment, and constant temperature cultivation can be achieved. The light organism reaction device has the advantages of being moderate in cultivation, good in liquid mixing, high in gas-liquid mass transfer intensity, low in shear force, quick in growth, intensive in biological cell density, capable of being harvested continuously and the like, so that the light organism reaction device has a high economic value and a high industrial application prospect.

Description

technical field [0001] The invention relates to a novel photobiological reaction device, which is suitable for the cultivation of microalgae and other photosynthetic organism cell suspensions. Background technique [0002] Microalgae is a kind of biological resource with great application value. The artificial cultivation of microalgae by photobioreactor is an important way to solve the large-scale cultivation and amplification of microalgae. [0003] At present, there are mainly two types of closed photobioreactors: pipeline type and crate type. In the two types of photoreactors, the circulation of the culture medium adopts two methods: pump and air lift, because the shearing effect of the air lift cycle on the cells is relatively large. Small, while providing algae fluid flow, it also facilitates gas exchange. [0004] Chinese patent CN1472305A reports an integrated airlift photobioreactor, which includes a tank, a riser, and a gas distributor, wherein the cylindrical ris...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M3/00
CPCC12M33/22C12M23/06C12M31/02C12M21/02C12M23/02C12M41/22
Inventor 张今令徐云鹏刘中民孙新德白长敏黄为董兴隆
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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