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[0004] Although it has been reported in China in recent years that optimizing the bacterial community structure can further improve the leaching efficiency of chalcopyrite, due to the presence of a large amount of iron ions in the leaching system, a large amount of jarosite will inevitably be produced during the leaching process. The denseness of the precipitate is very good, and it will be tightly wrapped on the mineral surface, which prevents the contact between microorganisms and the mineral surface, and affects the leaching efficiency
Recent studies have pointed out that controlling the lower pH (<1.5) will reduce the formation of jarosite precipitation, but the optimum pH of common leaching bacteria, such as Thiobacillus ferrooxidans, is 1.8-2.5, and too high acid will reduce Bacterial concentration during leaching, thereby reducing leaching efficiency
Method used
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Embodiment 1
[0017] Example 1 strain screening
[0018] In this example, 100mL of the wet leaching heap of the Zijinshan Copper Mine in Shanghang County, Fujian Province was taken, and the large particles were first filtered with filter paper, and then the filtrate was added to a 250ml Erlenmeyer flask containing 1% chalcopyrite, at 30°C, 170r / h min, carry out enrichment culture for 4 days, repeat 3-4 times, use Starky-Na 2 S 2 o 3 ·5H 2 O medium for plate separation, after 2 weeks, colonies are formed, and single bacteria are picked and dropped into Starky-S 0 The culture medium was cultured, and the plate separation was repeated 4 times to obtain pure bacteria.
Embodiment 2
[0019] Example 2 strain identification
[0020] The entire genome of the pure culture was extracted using the Bacterial Whole Genome Rapid Extraction Kit, and PCR was performed with 16S rDNA universal primers to identify the strain as acidophilic Thiobacillus.
Embodiment 3
[0021] Physiology, biochemistry and resistance research of the bacterial strain of embodiment 3
[0022] The morphology of the above-mentioned ZJJN was observed by transmission electron microscopy as follows: figure 1 . Take 10°C, 20°C, 25°C, 30°C and 45°C for the culture of the strains screened to determine the optimum growth temperature is 30°C; take initial pH 0.5, 0.8, 1.0, 1.5, 2.0 and 2.5 at the optimum The culture is carried out at a temperature, and the optimum growth pH range is determined to be 0.5-1.5. Determine the optimal energy substrate (g / l) S for the strain ZJJN by the following combination 0 10.0g, Na 2 S 2 o 3 ·5H 2 O10.0; FeSO 4 ·7H 2 O10.0; FeCl 2 10.0; Peptone 10.0; Yeast Extract 10.0; Glucose 10.0; Fructose, 10.0;
[0023] Using several antibiotics with a concentration gradient of 10 and 100mg / l, it was found that the sensitivity order of ZJJN to several common antibiotics was tetracycline > streptomycin > erythromycin > chloramphenicol > kanam...
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Abstract
The invention discloses an extreme acidophilus thiobacillus, which is named as Acidithiobacillus sp. ZJJN with the preservation number of CCTCC NO: M 2012104. The extreme acidophilus thiobacillus has strong susceptibility on various antibiotics and high tolerance on several common metal bodies in a biological infusion process. In addition, the extreme acidophilus thiobacillus and ferrous oxide thiobacillus synergetically conduct copper pyrites infusion tests under extreme potential of hydrogen (pH), compared with a reference system, it is discovered that recovery rate of copper is greatly improved. A scanning electron microscope is adopted to observe the surfaces of infused copper pyrites, jarosite on the surfaces of the copper pyrites in a system added ZJJN is greatly reduced, and infusion efficiency is greatly improved. The extreme acidophilus thiobacillus can resist low pH environment, is suitable to an infusion system of the infused copper pyrites, and is hopeful for use in industrial infused copper pyrites.
Description
technical field [0001] The invention relates to a strain of acidophilic thiobacillus, in particular to a strain of acidophilic thiobacillus and application thereof. Background technique [0002] Bioleaching is an interdisciplinary subject in the field of biology and metallurgy, also known as biooxidation or biohydrometallurgy, that is, using microorganisms, air and water and other natural substances to leach lean ore, waste ore, tailings and smelting slag, etc., to recycle Certain precious non-ferrous metals and rare metals are a metallurgical method to prevent the loss of mineral resources and maximize the use of mineral deposits; compared with traditional smelting methods, bioleaching usually has less infrastructure investment, low operating costs, and less environmental pollution. Lower-grade minerals can be processed, so it is considered a green metallurgical technology. [0003] Primary sulfide copper ore chalcopyrite is the most stable and difficult copper mineral to ...
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