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Method for extracting protein and chitin by fermenting shrimp heads and shrimp shells

A chitin and protein technology, applied in protein food ingredients, fish protein ingredients, food science and other directions, can solve the problems of loss of nutrients, pollution, low treatment efficiency, etc., to eliminate fishy odor, reduce environmental pollution, reduce effect of dosage

Active Publication Date: 2015-01-21
上海红马饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the problems of severe pollution, loss of nutrients and low processing efficiency in the existing method for extracting protein and chitin from aquatic products, and provides a method of fermenting shrimp head and chitin The method of extracting protein and chitin from shrimp shell

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] In this example, Lactobacillus helveticus is used to ferment and extract protein and chitin from shrimp heads and shrimp shells. The specific steps are as follows:

[0036] (1) Get 50g of fresh shrimp heads and shrimp shells, crush the shrimp heads and shrimp shells to about 10 mesh, add 25mL of water (50% of the total mass of the shrimp heads and shrimp shells), and measure the pH to 6.4;

[0037] (2) Add 7.5g glucose (15% of the total mass of the shrimp head and the shrimp shell), and 0.5g of yeast powder (1% of the total mass of the shrimp head and the shrimp shell);

[0038] (3) Lactobacillus helveticus is inoculated, and the inoculation amount is 10% of the total mass of the shrimp head and the shrimp shell;

[0039] (4) Insulated and fermented at 37°C for 3 days;

[0040] (5) After the fermentation, the fermentation broth and fermentation residue were centrifuged; the fermentation broth was neutralized by sodium carbonate, concentrated, and spray-dried to obtain ...

Embodiment 2

[0043] In this example, Lactobacillus helveticus is used to ferment and extract protein and chitin from shrimp heads and shrimp shells. The specific steps are as follows:

[0044] (1) Take 100g of fresh shrimp heads and shrimp shells, crush the shrimp heads and shrimp shells to about 10 meshes, and measure the pH of the joints to 6.3;

[0045] (2) Add 20g of glucose (20% of the total mass of the shrimp head and the shrimp shell), 2g of yeast powder (2% of the total mass of the shrimp head and the shrimp shell);

[0046] (3) Lactobacillus helveticus is inoculated, and the inoculation amount is 5% of the total mass of the shrimp head and the shrimp shell;

[0047] (4) Insulated and fermented at 37°C for 3 days;

[0048] (5) After the fermentation is finished, centrifuge the fermented liquid and the fermentation residue; the fermented liquid is neutralized by sodium carbonate, concentrated, and spray-dried to obtain 14.9 g of powdered substance, which is the protein obtained in ...

Embodiment 3

[0051] In this embodiment, protein and chitin are produced from shrimp heads and shrimp shells by fermentation of mixed strains, and the specific steps are as follows:

[0052] (1) Get 50g of dried shrimp heads and shrimp shells, crush the shrimp heads and shrimp shells to about 10 meshes, add 25mL of water (50% of the total mass of the shrimp heads and shrimp shells), and measure the pH to 6.4;

[0053] (2) add 7.5g glucose again (15% of mass of shrimp head and shrimp shell), yeast powder of 1g (2% of total mass of shrimp head and shell);

[0054] (3) Inoculation of Lactobacillus helveticus: Lactobacillus bulgaricus=1: 1, the inoculation amount is 10% of the total mass of shrimp head and shrimp shell;

[0055] (4) Insulated and fermented at 42°C for 3 days;

[0056] (5) After the fermentation finishes, centrifuge the fermented liquid and the fermented residue; the fermented liquid is extracted with ethanol to produce astaxanthin, and the extracted aqueous phase is neutralize...

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Abstract

The invention discloses a method for extracting protein and chitin by fermenting shrimp heads and shrimp shells. The method comprises the following steps of: (1) adding a carbon source and a nitrogen source in the shrimp heads and shrimp shells which are crushed or homogenized to be 10-50 meshes, and then uniformly mixing; (2) inoculating lactobacillus helveticus or a mixed bacterial strain; (3) performing heat-insulating fermentation; (4) separating the fermentation broth from residue after the fermentation is concluded; (5) drying the fermentation broth to obtain protein powder; and (6) soaking the fermentation residue obtained in the step (4), and decolouring and fermenting to obtain chitin. With the adoption of the method disclosed by the invention, the protein powder without fishiness and rich in calcium lactate, and chitin can be obtained; and the method is rapid, simple, and capable of reducing production cost, reducing environmental pollution, and adequately utilizing rich nutritional ingredients in the shrimp heads and the shrimp shells.

Description

technical field [0001] The invention specifically relates to a method for extracting protein and chitin by fermenting shrimp heads and shrimp shells. Background technique [0002] For shrimps, shrimp heads and shells are rich in nutrients. For example, the shrimp heads and shells of shrimps account for about 30% of the shrimp body weight. In addition to minerals such as chitin and calcium carbonate, they also contain 7-15% protein, 2-4% oil, rich in vitamin E, lutein, lecithin and other ingredients, with high nutritional value. [0003] At present, the utilization of shrimp heads and shrimp shells is mainly in two ways: one is to produce chitin, and the other is to use self-cathepsin or exogenous protease to hydrolyze the protein therein and liquefy it to separate water. However, direct production of chitin from shrimp heads and shells requires high-concentration acid and alkali fermentation raw materials, which not only seriously pollutes, but also removes protein, fat, ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/04C08B37/08
Inventor 刘国庆赵肖胡卫华钱晓勇
Owner 上海红马饲料有限公司
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