Straw-decomposition composite microbial preparation and preparation method thereof
A technology of composite microorganisms and rice straw, which is applied in the field of rice straw decomposing composite microbial preparations and its preparation, to achieve the effects of reducing investment, facilitating migration, and improving soil physical and chemical properties
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Embodiment 1
[0039] Example 1: A kind of rice straw decomposing compound microbial preparation, it is formulated by following mass percent raw material:
[0040] bacteria mass percentage bacteria mass percentage Achromobacter xylosoxidans 12% Bacillus megaterium 8% Bacillus welcheei 14% staphylococcus 10% Bacillus amyloliquefaciens 12% Aspergillus niger 44%
[0041] The Aspergillus niger, Achromobacter xyloseoxidans, Bacillus welschii, Bacillus amyloliquefaciens, Bacillus megaterium and Staphylococcus in the table are all preserved in the General Microorganism Center of China Committee for Culture Collection of Microorganisms, referred to as CGMCC.
[0042] A preparation method of rice straw decomposing compound microbial preparation, the steps are:
[0043] 1. Separate expansion culture of each strain:
[0044] 1) Cultivation of Aspergillus niger: Inoculate Aspergillus niger on potato medium (refer to microbiology experiment textbook) at a...
Embodiment 2
[0053] A kind of rice straw decomposing compound microbial preparation, it is formulated by following mass percent raw material:
[0054] bacteria mass percentage bacteria mass percentage Achromobacter xylosoxidans 20% Bacillus megaterium 15% Bacillus welcheei 10% staphylococcus 5% Bacillus amyloliquefaciens 20% Aspergillus niger 30%
[0055] The Aspergillus niger, Achromobacter xyloseoxidans, Bacillus welschii, Bacillus amyloliquefaciens, Bacillus megaterium and Staphylococcus in the table are all preserved in the General Microorganism Center of China Committee for Culture Collection of Microorganisms, referred to as CGMCC.
[0056] Its preparation steps are identical with embodiment 1.
test example 1
[0057] Test case 1: The rice straw decomposing compound microbial preparation test was carried out in Yangxi Village, Wenzhen Town, Jinxian County, Jiangxi Province in the late season of 2009. The pH value of the tested soil is 6.5, and the organic matter content is 42.3 g·kg -1 , total nitrogen 1.87 g·kg -1 , alkaline hydrolyzed nitrogen 151 mg·kg -1 , available phosphorus 6.0mg·kg -1 , available potassium 178 mg·kg -1 . The previous stubble was early rice, and after the early rice was harvested, it was machine-cut and returned to the field in full. The rice straw was cut into 10 cm length by the harvester, and then transplanted by rotary tillage.
[0058] Four treatments were set up in the experiment: (1) Control without rice straw returning to the field, that is, all the early rice straw after mechanical harvesting was removed from the test field; (2) Full amount of rice straw was returned to the field; (3) Full amount of rice straw was returned to the field + rice st...
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