Method for preparing xylooligosaccharide by using mulberry branches
A technology of xylooligosaccharides and mulberry branches, applied in the fields of food engineering and biochemical industry, to achieve the effect of expanding raw material sources, stable physical and chemical properties, great social significance and economic value
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Embodiment 1
[0028] Take mulberry branches, wash them to remove impurities such as soil, peel them, dry them at 120° C., and crush them into small pieces smaller than 0.5 cm to obtain mulberry branch powder.
[0029] Add mulberry powder to NaOH aqueous solution with a mass percentage concentration of 1%, the dosage ratio of the mulberry powder to NaOH aqueous solution is 1Kg:8L, activate at 80°C for 6h, filter, add ethanol twice the volume of NaOH aqueous solution to obtain Precipitation, precipitation and drying are xylan residues.
[0030] The xylan residue is mixed with xylanase, the dosage of xylanase is 800IU / g xylan, the pH is adjusted to 5.0, and the enzyme is hydrolyzed at 37°C for 10h. Heat at 80°C for 20 minutes to inactivate xylanase, add activated carbon and mix at 80°C for 20 minutes, remove the activated carbon by filtration; desalt with a column filled with cation exchange resin BK001, filter with filter paper to remove solid impurities, and freeze-dry to obtain xylooligosac...
Embodiment 2
[0033] Take mulberry branches, wash them to remove impurities such as soil, peel them, dry them at 120° C., and crush them into small pieces smaller than 0.5 cm to obtain mulberry branch powder.
[0034] Add the mulberry branch powder to the NaOH aqueous solution with a mass percentage concentration of 10%, the dosage ratio of the mulberry branch powder and the NaOH aqueous solution is 1Kg: 6L, activate it at 90°C for 3h, remove the residue by filtration, and add 3 times the volume of the NaOH aqueous solution Ethanol is precipitated, and the precipitate is dried to become xylan residue.
[0035] The xylan residue is mixed with xylanase, the dosage of xylanase is 800IU / g xylan, the pH is adjusted to 4.5, and the enzyme is hydrolyzed at 50°C for 5h. Heat at 80°C for 20 minutes to inactivate xylanase, add activated carbon to mix and decolorize at 80°C for 20 minutes, and filter to remove the activated carbon. Use a column equipped with cation exchange resin BK001 for desalting,...
Embodiment 3
[0037] Take mulberry branches, wash to remove soil and other impurities, peel them, dry them at 100°C, and crush them into small pieces smaller than 0.5 cm to obtain mulberry branch powder.
[0038] Add the mulberry branch powder to the KOH aqueous solution with a mass percent concentration of 5%, the dosage ratio of the mulberry branch powder to the KOH aqueous solution is 1Kg:10L, activate it in an ultrasonic field at 90°C for 4 hours, filter to remove the residue, and add the KOH aqueous solution Two times the volume of ethanol was precipitated, and the precipitate was dried to obtain xylan residue.
[0039] The xylan residue is mixed with xylanase, the dosage of xylanase is 1000 IU / g xylan, the pH is adjusted to 5.0, and the enzyme is hydrolyzed at 40° C. for 8 hours. Heat at 80°C for 20 minutes to inactivate xylanase, decolorize and remove impurities with anion exchange resin D-268, desalt with cation exchange resin BK001, filter through filter paper to remove solid impur...
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