Preparation method of agarose immune magnetic microspheres and applications thereof
A technology of magnetic microspheres and agarose, which is applied in the preparation of microspheres, anti-animal/human immunoglobulins, microcapsule preparations, etc., which can solve the problems of complex monoclonal antibody process, highly toxic coupling agent, and high cost , to achieve the effect of reducing pretreatment time, low corrosion, and easy operation
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specific Embodiment approach 1
[0028] Specific embodiment one: the preparation method of a kind of agarose immunomagnetic microsphere of the present embodiment is to carry out according to the following steps:
[0029] 1. Take 10-15g of FeCl3·6H 2 O and 30-50g of sodium oleate are dissolved in a mixed solvent, heated to 60-80°C, and kept for 4-6 hours. After the reaction is completed, take the upper organic solvent and wash it with distilled water three times, and remove the organic solvent by rotary evaporation to obtain ferric oleate. Precursor; wherein, the mixed solvent is formed by mixing ethanol, distilled water and n-hexane in a volume ratio of 60-80:40-60:120-140;
[0030] 2. At room temperature, mix 30-40g of oleic acid, 5-8g of the iron oleate precursor obtained in step 1, and 100-200g of octadecene, and then heat it to 320°C at a rate of 3-5°C per minute. ~340℃, keep warm for 20~50min, then naturally cool to room temperature, add 400~600mL of absolute ethanol, and centrifuge at 3000~5000rpm for ...
specific Embodiment approach 2
[0041] Embodiment 2: This embodiment differs from Embodiment 1 in that: in step 1, heat to 70° C. and keep warm for 4 hours. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0042] Embodiment 3: This embodiment is different from Embodiment 1 or 2 in that: in step 1, the mixed solvent is formed by mixing ethanol, distilled water and n-hexane in a volume ratio of 80:60:140. Others are the same as in the first or second embodiment.
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