Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extracting-separating method of polypodium glycyrrhiza A

A technique for the separation of myriapodendron and its application in steroids, organic chemistry, etc., to achieve the effects of improving extraction rate, reducing sample loss, and high efficiency

Inactive Publication Date: 2013-08-07
NANJING ZELANG AGRI DEV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no relevant literature and patent reports on the extraction and purification of myriapodinaside A by high-speed countercurrent chromatography in China.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The rhizome raw material of sweet-rooted fern was crushed, passed through a 40-mesh sieve, weighed 3kg, added 30g of pectinase, mixed evenly, and enzymatically hydrolyzed for 3 days, and the enzymolyzed raw material was cavitated and suspended for 50min with 24L of 70% ethanol solution, and carried out negative Suspended solid-liquid extraction by pressure cavitation once to obtain extract, concentrated to extract, add 800ml water to disperse, add 800ml water-saturated n-butanol to extract twice, combine extracts, add 1600ml acetone to precipitate, filter, and concentrate the filtrate Dry to obtain the crude product; use petroleum ether-ethyl acetate-ethanol-water (5:2:3:3) as a two-phase solvent system, mix well, put it in a separatory funnel and let it stand for stratification, take the upper phase as the stationary phase, The lower phase is the mobile phase, and the crude powder is dissolved in the lower phase, then the upper phase is used to pump the chromatographic ...

Embodiment 2

[0021] The raw material of the rhizome of sweet-rooted fern is crushed, passed through a 60-mesh sieve, weighed 3kg, added 30g of amylase, mixed evenly, and enzymatically hydrolyzed for 2 days, and the enzymolyzed raw material was cavitated and suspended for 40min with 30L of 75% ethanol solution, and carried out under negative pressure Cavitation suspended solid-liquid extraction twice to obtain the extract, concentrate to the extract, add 900ml water to disperse, add 1000ml water-saturated n-butanol to extract twice, combine the extracts, add 1200ml acetone to precipitate, filter, and the filtrate is concentrated and dried The crude product was obtained; petroleum ether-ethyl acetate-ethanol-water (6:3:5:4) was used as a two-phase solvent system, mixed evenly and placed in a separatory funnel to stand for stratification, the upper phase was taken as the stationary phase, and the lower phase was used as the stationary phase. The phase is the mobile phase, the crude powder is d...

Embodiment 3

[0023] The rhizome raw material of sweet-rooted fern was crushed, passed through a 20-mesh sieve, weighed 3kg, added 30g of cellulase, mixed evenly, and enzymatically hydrolyzed for 2 days, and the enzymolyzed raw material was cavitated and suspended for 50min with 15L of 85% ethanol solution, and carried out negative Suspended solid-liquid extraction by pressure cavitation twice to obtain extract, concentrated to extract, add 800ml water to disperse, add 500ml water-saturated n-butanol to extract 4 times, combine extracts, add 1200ml acetone to precipitate, filter, and concentrate the filtrate Dry to obtain the crude product; use petroleum ether-ethyl acetate-ethanol-water (8:3:3:2) as a two-phase solvent system, mix well, put it in a separatory funnel and let it stand for stratification, take the upper phase as the stationary phase, The lower phase is the mobile phase, and the crude powder is dissolved in the lower phase, then the upper phase is used to pump the chromatograph...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an extracting-separating method of polypodium glycyrrhiza A. The extracting-separating method of the polypodium glycyrrhiza comprises the following steps of: crushing stems and roots of polypodium glycyrrhiza; adding biological enzyme for carrying out enzymolysis for 1-3 days; adding 70%-95% alcohol solution 5-7 times the amount of the materials for mixing; carrying out negative-pressure cavitation suspension solid-liquid extraction to obtain an extracting solution; concentrating until extract is obtained; adding a proper amount of water for dispersing; adding water-saturated n-butyl alcohol for extracting for 2-4 times, combining the extracting solution, adding acetone for settling and filtering; concentrating and drying the filtrate to obtain a crude product; and separating and purifying the crude product by adopting a high-speed counter current chromatography to obtain the polypodium glycyrrhiza A. the extracting-separating method is large in preparation amount, less in sample loess and high in product content.

Description

technical field [0001] The invention belongs to the field of separation of natural products, and in particular relates to an extraction and separation method of myriapodinaside A. Background technique [0002] Polypodoside A is a steroidal saponin with the molecular formula C 45 h 72 o 17 , molecular weight 885.05. Scleroside A can be used as a sweetener, and its sweetness is 600 times that of 6% (w / v) sucrose solution. Myriapodiaceae mainly comes from the sweet root Myriapodiaceae of Polypodiaceae Polypodium glycyrrhiza Rhizome of DC. Eaton. At present, the large-scale development of natural sweeteners that are non-toxic, safe to use, low in calories, high in quality and have health effects to replace sucrose and synthetic sweeteners is a major issue that scientific and technological workers at home and abroad urgently need to solve. [0003] At present, there are no relevant literature and patent reports on the extraction and purification of myriapodinaside A by hig...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07J17/00
Inventor 苏刘花万冬梅
Owner NANJING ZELANG AGRI DEV
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com