Extracting-separating method of polypodium glycyrrhiza A

A technique for the separation of myriapodendron and its application in steroids, organic chemistry, etc., to achieve the effects of improving extraction rate, reducing sample loss, and high efficiency

Inactive Publication Date: 2013-08-07
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no relevant literature and patent reports on the extraction a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1:

[0019] The rhizome raw materials of Pteridophyta are crushed, pass through a 40-mesh sieve, weigh 3 kg, add 30 g of pectinase and mix evenly, enzymatically hydrolyze for 3 days, add 24L of 70% ethanol solution to the enzymatic hydrolysis raw materials and cavitate for 50 minutes. The solid-liquid extraction was performed once by cavitation to obtain the extract, which was concentrated to extract, dispersed with 800 ml of water, and extracted twice with 800 ml of water-saturated n-butanol. Combine the extracts, add 1600 ml of acetone to precipitate, filter, and concentrate the filtrate. Dry to obtain the crude product; use petroleum ether-ethyl acetate-ethanol-water (5:2:3:3) as a two-phase solvent system, mix well, place it in a separatory funnel and let it stand for stratification, and take the upper phase as the stationary phase, The lower phase is the mobile phase, the crude powder is dissolved in the lower phase, and then the upper phase is used to pump...

Example Embodiment

[0020] Example 2:

[0021] The rhizome raw material of Pteridophyta is pulverized, passed through a 60-mesh sieve, weighed 3kg, mixed with 30g of amylase, and enzymatically hydrolyzed for 2 days. The enzymolysis raw material was added with 30L of 75% ethanol solution for cavitation suspension for 40min, and the negative pressure was carried out. The cavitation suspension was extracted twice to obtain the extract, which was concentrated to the extract, dispersed with 900ml of water, and extracted twice with 1000ml of water-saturated n-butanol. The crude product was obtained; the petroleum ether-ethyl acetate-ethanol-water (6:3:5:4) was used as the two-phase solvent system, and the mixture was placed in a separatory funnel and left to stand for stratification. The upper phase was taken as the stationary phase, and the lower The phase is the mobile phase, the crude product powder is dissolved in the lower phase, and then the upper phase is used to pump the chromatographic column,...

Example Embodiment

[0022] Example 3:

[0023] The rhizome raw materials of Pteridophyta are crushed, pass through a 20-mesh sieve, weigh 3 kg, add 30 g of cellulase and mix evenly, enzymatically hydrolyze for 2 days, add 15 L of 85% ethanol solution to the enzymatic hydrolysis raw materials and suspend for 50 min. The suspension was extracted twice by cavitation to obtain the extract, which was concentrated to the extract, dispersed with 800 ml of water, and extracted 4 times with 500 ml of water-saturated n-butanol. The extracts were combined, precipitated by adding 1200 ml of acetone, filtered and concentrated. Dry to obtain the crude product; use petroleum ether-ethyl acetate-ethanol-water (8:3:3:2) as the two-phase solvent system, mix well, place it in a separatory funnel and let it stand for layers, take the upper phase as the stationary phase, The lower phase is the mobile phase, the crude powder is dissolved in the lower phase, and then the upper phase is used to pump the chromatographic ...

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Abstract

The invention relates to an extracting-separating method of polypodium glycyrrhiza A. The extracting-separating method of the polypodium glycyrrhiza comprises the following steps of: crushing stems and roots of polypodium glycyrrhiza; adding biological enzyme for carrying out enzymolysis for 1-3 days; adding 70%-95% alcohol solution 5-7 times the amount of the materials for mixing; carrying out negative-pressure cavitation suspension solid-liquid extraction to obtain an extracting solution; concentrating until extract is obtained; adding a proper amount of water for dispersing; adding water-saturated n-butyl alcohol for extracting for 2-4 times, combining the extracting solution, adding acetone for settling and filtering; concentrating and drying the filtrate to obtain a crude product; and separating and purifying the crude product by adopting a high-speed counter current chromatography to obtain the polypodium glycyrrhiza A. the extracting-separating method is large in preparation amount, less in sample loess and high in product content.

Description

technical field [0001] The invention belongs to the field of separation of natural products, and in particular relates to an extraction and separation method of myriapodinaside A. Background technique [0002] Polypodoside A is a steroidal saponin with the molecular formula C 45 h 72 o 17 , molecular weight 885.05. Scleroside A can be used as a sweetener, and its sweetness is 600 times that of 6% (w / v) sucrose solution. Myriapodiaceae mainly comes from the sweet root Myriapodiaceae of Polypodiaceae Polypodium glycyrrhiza Rhizome of DC. Eaton. At present, the large-scale development of natural sweeteners that are non-toxic, safe to use, low in calories, high in quality and have health effects to replace sucrose and synthetic sweeteners is a major issue that scientific and technological workers at home and abroad urgently need to solve. [0003] At present, there are no relevant literature and patent reports on the extraction and purification of myriapodinaside A by hig...

Claims

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Application Information

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IPC IPC(8): C07J17/00
Inventor 苏刘花万冬梅
Owner NANJING ZELANG AGRI DEV
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