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Preparation method of water-soluble rare earth up-converting luminescence nano particle modified by amino acid

A rare earth upconversion and nanoparticle technology, applied in luminescent materials, chemical instruments and methods, etc., can solve the problems of reduced luminous intensity of upconversion nanoparticles, limited application, easy aggregation, etc., to avoid complex organic synthesis, easy to operate and Easy to master and prepare

Inactive Publication Date: 2013-08-07
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the present invention: the prior art has the following deficiencies: the oil-soluble rare earth up-conversion nanoparticles prepared in the organic phase have high luminous intensity, and the shape and size of the particles are uniform, but they are easy to aggregate in aqueous solution, which limits their various properties in aqueous solution. Although there have been reports on the preparation methods of water-soluble up-conversion nanoparticles, the luminous intensity of the up-conversion nanoparticles prepared by these methods is often greatly reduced in aqueous solution, the dispersibility is still not ideal, and the surface of the up-conversion nanoparticles lacks Active functional groups available for coupling, not directly applicable to various biological applications

Method used

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  • Preparation method of water-soluble rare earth up-converting luminescence nano particle modified by amino acid
  • Preparation method of water-soluble rare earth up-converting luminescence nano particle modified by amino acid
  • Preparation method of water-soluble rare earth up-converting luminescence nano particle modified by amino acid

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Embodiment 1

[0020] Example 1: Lysine-modified rare earth upconversion (NaYF 4 : Yb / Er) nanoparticles preparation

[0021] Oleic acid modified NaYF 4 : Yb / Er upconversion nanoparticles (NaYF 4 : Yb / Er) preparation: add 3mL oleic acid and 17mL octadecene respectively in a 100mL three-necked flask, then add 0.78mmolYCl 3 ·6H 2 O, 0.20 mmol YbCl 3 ·6H 2 O, 0.02 mmol ErCl 3 ·6H 2 O, in N 2 Stir for 30min under protection, heat up to 160°C, the solution turns into a yellow, clear and transparent solution, then cool down to room temperature, add NH 4 Methanol solution of F (0.148gNH 4 F and 0.1g NaOH dissolved in 10mL methanol) 10mL, heated to 80°C and kept for 30min, then raised to 300°C, reacted for 1h, cooled to room temperature naturally, discarded the upper liquid, added ethanol and ethanol / water to the precipitated part (nanocrystal) (1:1, v / v) washing, repeated 3 times, the obtained solid was dried in an oven at 70°C, and stored in a desiccator for future use.

[0022] Oleic ac...

Embodiment 2

[0025] Embodiment 2: Glycine-modified rare earth up-conversion nanoparticles (NaYF 4 : Yb / Er) preparation

[0026] Oleic acid modified NaYF 4 : The preparation of Yb / Er up-conversion nanoparticles and the removal of surface oleic acid molecules are the same as in Example 1

[0027] Glycine-modified NaYF 4 : Preparation of Yb / Er up-conversion nanoparticles: the obtained NaYF with oleic acid removed 4 : 0.1 g of Yb / Er up-converting nanoparticles was redispersed in 10 mL of water, 1 mL of 100 μM glycine aqueous solution was added, and stirred for 3 hours. The obtained water-soluble nanocrystals were washed with ethanol and water, dried in an oven at 70° C., and stored in a desiccator for future use. Figure 5 is glycine-modified NaYF 4 : Comparison of the solubility of Yb / Er upconverting nanoparticles in cyclohexane and aqueous solution. Glycine-modified nanoparticles precipitated in cyclohexane but were clear in aqueous solution, indicating that glycine-modified NaYF 4 : Y...

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Abstract

The invention relates to a preparation method of a water-soluble rare earth up-converting luminescence nano particle modified by amino acid. The method is characterized by comprising the following steps: removing oleic acid on the surface of the NaYF4:Yb / Er up-converting nano particle by using a solvent extraction method in the acidic environment with pH 3-5 on the basis of the NaYF4:Yb / Er up-converting nano particle which is modified by oleic acid and has high light intensity; and functionalizing the rare earth up-converting particle without oleic acid by using amino acid to obtain the water-soluble rare earth up-converting luminuscence nano particle. The prepared rare earth up-converting nano particle not only has the high light intensity of the NaYF4:Yb / Er up-converting nano particle modified by oleic acid, but also is completely water-soluble, and the surface of the rare earth up-converting nano particle has amino groups and carboxyl groups which can be used for being crosslinked with biomolecules. The preparation method is simple and easy to operate and has a good application prospect.

Description

technical field [0001] The invention relates to a preparation method of water-soluble rare earth up-conversion luminescent nanoparticles. The prepared up-conversion luminescent nanoparticles can be used for biological marking, sensing and imaging, and belong to the field of preparation of rare earth up-conversion luminescent nano-materials. Background technique [0002] Up-conversion luminescence is the luminescence that emits short-wavelength light after being excited by absorbing multiple photons of long-wavelength radiation. Compared with commonly used luminescent markers such as fluorescent dye molecules and quantum dots, rare earth up-conversion luminescent materials have the advantages of large Stokes shift, high luminous intensity, and low toxicity to organisms. The excitation light source is a near-infrared light source (usually 980nm) , to avoid the interference of various background fluorescence of biological samples caused by ultraviolet light excitation. In addit...

Claims

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Application Information

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IPC IPC(8): C09K11/85
Inventor 陈扬刘保霞
Owner SOUTHEAST UNIV
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