Hybrid overall crystal gel medium and preparation method thereof

A crystal-colloid medium and mixed technology, applied in chemical instruments and methods, other chemical processes, anion exchange, etc., can solve the problems of single and lack of functional groups, achieve good channel connectivity, high porosity, and improve separation performance effect

Active Publication Date: 2013-08-21
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anion-exchange crystal colloid media in the existing literature have a single functional group, and there is a lack of hybrid overall crystal colloid media with certain hydrophobic functions and ion exchange functions.

Method used

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  • Hybrid overall crystal gel medium and preparation method thereof
  • Hybrid overall crystal gel medium and preparation method thereof
  • Hybrid overall crystal gel medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take a polyacrylamide-based monolithic gel matrix with a diameter of 10 mm and a height of 69 mm, and use 27 mL of K with a concentration of 0.056 M 5 [Cu(HIO 6 ) 2 ] aqueous solution as the catalyst, using 27mL of 0.25M N,N,N-trimethylvinylbenzyl ammonium chloride aqueous solution as the reaction solution, grafting reaction at 40°C for 4h, to obtain a super-macroporous hybrid monolithic gel Separation medium (i.e. a hybrid monolithic colloidal medium with hydrophobic benzyl-anion-exchange tertiary amino groups), the effective porosity is 87%, the maximum porosity is 94%, and the pore size is about 1-270 μm. The scanning electron microscope picture of its microstructure is shown in figure 1 ;Water phase permeability 6×10 -12 m 2 ;The dynamic adsorption capacity for bovine serum albumin (breakthrough concentration / sample concentration>0.9, the same below) at a flow rate of 2cm / min reaches 0.8mg / mL, and the static adsorption capacity for bovine serum albumin reaches 2....

Embodiment 2

[0021] Take a polyacrylamide-based monolithic gel matrix with a diameter of 10 mm and a height of 76 mm, and use 17.9 mL of K with a concentration of 0.056 M 5 [Cu(HIO 6 ) 2 ] aqueous solution as a catalyst, using 16 mL of N,N,N-trimethylvinylbenzyl ammonium chloride aqueous solution with a concentration of 1M as a reaction solution, grafting reaction at 50°C for 2 hours, to obtain a hydrophobic benzyl-anion-exchange tertiary Amino mixed overall ultra-large pore crystal gel separation medium, with an effective porosity of 85%, a maximum porosity of 94%, and a water phase permeability of 2×10 -12 m 2 The pore size is about 1-300 μm, the dynamic adsorption capacity for bovine serum albumin reaches 2.3 mg / mL at a flow rate of 1 cm / min, and the static adsorption capacity for bovine serum albumin reaches 3.5 mg / mL. The medium has elasticity, good mechanical strength, and no obvious deformation under high flow rate (such as 10-15cm / min, 20mM pH7.2 phosphate buffer solution); it c...

Embodiment 3

[0023] Take a polyhydroxyethyl methacrylate monolithic gel matrix with a diameter of 10 mm and a height of 80 mm, and use 19 mL of K with a concentration of 0.037 M 5 [Cu(HIO 6 ) 2 ] aqueous solution as a catalyst, using 6.3mL of 0.5M N,N,N-trimethylvinylbenzyl ammonium chloride aqueous solution as a reaction solution, grafting reaction at 55°C for 0.5h, to obtain a hydrophobic benzyl- Anion-exchange tertiary amino group hybrid overall ultra-large-porous crystal gel separation medium, with an effective porosity of 86%, a maximum porosity of 96%, a pore size of about 20-210 μm, and a water phase permeability of 3×10 -12 m 2 ; At a flow rate of 2cm / min, the dynamic adsorption capacity for bovine serum albumin reaches 1.2mg / mL, and the static adsorption capacity for bovine serum albumin reaches 2.8mg / mL. The medium has elasticity, good mechanical strength, and no obvious deformation under high flow rate (such as 10-15cm / min, 20mM pH7.2 phosphate buffer solution); it can recove...

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Abstract

The invention discloses a hybrid overall crystal gel separation medium with an ultra-large hole having hydrophobic benzyl-anion exchange tertiary amine and a preparation method of the hybrid overall crystal gel separation medium. The aperture of the crystal gel medium is 1-300 microns, the porosity is 85-96 percent, and the aqueous phase permeability is 2*10<-12>-6*10<-12>m<2>; and the crystal gel medium is provided with a functional group of the hydrophobic benzyl-anion exchange tertiary amine shown in a formula (I). A crystal gel medium polymer chain provided by the hybrid overall crystal gel separation medium simultaneously contains an amino-type ion exchange group and benzyl having a certain hydrophobic function, so that multi-point absorption of the hybrid overall crystal gel medium and biological macromolecules of proteins and the like is facilitated, the separation performance is improved, and the medium has excellent basic functions of other crystal gel mediums, like high porosity and good pore canal connectivity, and a certain mechanical intensity function, like that the medium is quickly recovered into an original shape after the medium is dried and subjected to water absorption again; and the medium has wide application prospect in the biochemical separation field; and n is a positive integer in the formula (I).

Description

(1) Technical field [0001] The invention relates to a super-macroporous mixed monolithic gel separation medium and a preparation method thereof, in particular to a hybrid monolithic crystal gel medium with hydrophobic benzyl-anion-exchange tertiary amino groups and a preparation method thereof. (2) Background technology [0002] The crystal-gel separation medium has ultra-large pores ranging in size from a few microns to hundreds of microns, allowing complex fluids such as complex fermentation broth, culture fluid, or transformation fluid containing microbial cell fragments to pass directly through the bed, and can achieve rapid biomacromolecular substances Separation has important application prospects in the downstream field of biology. It is of great significance to study and develop new ion-exchange gel media with different functional groups. [0003] At present, there are few reports on mixed crystal-gel separation media at home and abroad, and there are few reports on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/30
CPCB01J41/14B01J20/28054B01J20/28085
Inventor 贠军贤林东强关怡新姚善泾沈绍传
Owner ZHEJIANG UNIV OF TECH
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