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A method and reaction system for simultaneously producing biogas and fatty acid

A fatty acid and biogas technology, applied in the method and its reaction system, simultaneously producing biofuels and important chemical products

Inactive Publication Date: 2014-10-29
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of selectively enriching hydrogenophilic methanogens while inhibiting the activity of acetophilic methanogens in the mixed flora, the object of the present invention is to provide a method to control the composition of the mixed flora by environmental conditions to simultaneously produce biogas and fatty acids Methods

Method used

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  • A method and reaction system for simultaneously producing biogas and fatty acid

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Effect test

Embodiment 1

[0032] Example 1 Reaction system for simultaneous production of biogas and fatty acid

[0033] Such as figure 1 As shown, a reaction system for simultaneous production of biogas and fatty acid includes: a reactor 1, and an organic substrate supply device 2 connected to the reactor 1, a medium supply device 3, a liquid phase metabolite recovery device 4, Gas phase metabolite recovery device 5, pH value detection device 6 and pH value control device. Wherein, the reactor 1 is provided with the organic substrate supply device 2, the liquid phase metabolite recovery device 4, the gas phase metabolite recovery device 5, the pH value detection device 6, and the pH value control device 7. Water inlet 12, water outlet 14, gas outlet 15, pH meter insertion port 16, and acid liquid sample inlet 17 and lye sample inlet 18; Described culture medium supply device 3 shares with described organic substrate supply device 2 The water inlet 12.

[0034] In this embodiment, the pH value detec...

Embodiment 2

[0040] The device used adopts the device in embodiment 1, such as figure 1shown. The material of the reactor is glass. The shell has an inner diameter of 12cm and a height of 17cm. A water inlet 12, a lower sampling port 13, an upper sampling port 13 and a water outlet 14 are respectively arranged at 2 cm, 6 cm, 11 cm and 12 cm from the lower end.

[0041] The fermentation method is as follows:

[0042] Add the inoculum and medium to the anaerobic fermentation reactor. The inoculum was anaerobic sludge from the treatment of citric acid wastewater by Anhui Fengyuan Biochemical Co., Ltd., which was a mixed flora containing Thermoanaerobacter tengcongensis (high-temperature-resistant bacteria) and Methanothermobacter thermautotrophicus (high-temperature-resistant archaea). The influent of the reactor is glucose with a concentration of 10g / L, and the HRT of the reactor is 20 days. The composition of the medium is (unit: mg / L) NH 4 Cl, 500; KH 2 PO 4 , 200; Na 2 SO 4 , 40...

Embodiment 3

[0049] Example 3 In the batch mode, the reactor system for the simultaneous production of biogas and fatty acid by controlling the composition of the mixed flora through environmental conditions adopts the same reaction system as in Example 1, and adopts the same reaction system as in Example 2 from Fengyuan Biochemical Co., Ltd. Anaerobic sludge from the treatment of citric acid wastewater was used as the inoculation source. The composition of the medium is (unit: mg / L) NH 4 Cl, 500; KH 2 PO 4 , 200; Na 2 SO 4 , 40; KCl, 50; CaCl 2 , 10; MgCl 2 ·6H 2 O, 70; MnCl 2 4H 2 O, 0.8; CoCl 2 2H 2 O, 1.2; FeSO 4 ·7H 2 O, 3.2; AlCl 3 , 0.5; NaMO 4 2H2O, 0.1; H 3 BO 3 , 0.2; NiCl 2 ·6H 2 O, 0.5; CuCl 2 2H 2 O, 1.1; ZnSO 4 2H 2 O, 3.2; EDTA (Na + type), 3.0. The pH value is maintained between 6.5-7.5 and the temperature is 70°C. Run in batch mode for 10 days, add glucose three times a day, its concentration is 6.0g / L. The concentration of acetic acid reaches 35g...

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Abstract

The invention provides a method for simultaneously producing biogas and fatty acid. The method comprises the following steps of placing anaerobic microorganisms and culture mediums into a fermentation system, and controlling the rotation speed, the pH value and the temperature of the fermentation system; placing an organic substrate into the fermentation system according to a sequencing batch mode, and gradually increasing the temperature of the reaction system until the temperature of the fermentation system reaches a critical temperature; maintaining the temperature constant, and continuing adding the organic substrate into the fermentation system according to a sequencing batch mode; and adding the organic substrate into the fermentation system according to a continuous flow mode. The invention also provides a reaction system for simultaneously producing biogas and fatty acid. By controlling the composition of a mixed flora through an environment condition, the gas metabolite and the liquid metabolite of the fermentation system can be simplified, the gas product, i.e. biogas of a reactor contains methane and carbon dioxide, and the liquid product is acetic acid, so that the simultaneous production of biofuel and important chemical products through the anaerobic fermentation of the mixed flora can be successfully realized.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for simultaneously producing biofuels and important chemical products and a reaction system thereof. Background technique [0002] Energy is one of the important foundations for maintaining human life and promoting social and economic development. Replacing fossil energy with renewable energy is one of the important ways to solve global problems such as fossil energy depletion and environmental degradation, and it is also a hot spot of current attention and research. Mixed flora anaerobic fermentation technology has the advantages of being able to utilize a variety of organic substrates, simple operation, and low operating costs. It can also produce a variety of important biofuels and industrial chemicals. Therefore, the anaerobic fermentation technology of mixed flora is one of the potential paths to solve the current energy and environmental problems. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/54C12P5/02C12M1/38C12M1/36C12M1/02C12R1/01
CPCY02E50/343Y02E50/30
Inventor 曾建雄张放张岩陈曼褚培娜丁静申晓菲
Owner UNIV OF SCI & TECH OF CHINA
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