Photon encoding microsphere-based multielement biomarker assay kit-type chip

A technology of biomarkers and encoded microspheres, which is applied in the field of multi-biomarker detection cassette chips based on photon encoded microspheres, can solve the problem that microspheres reduce the accuracy and repeatability of experimental data, and affect the accuracy and repeatability of methods. , uncontrollable experimental errors, etc., to achieve the effect of reduced accuracy, less sample requirements, and concise plan

Inactive Publication Date: 2013-08-28
南京环朋医疗技术有限公司
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  • Abstract
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Problems solved by technology

In the multiple detection repeated experiments based on photonic crystal-encoded microspheres, the difficulty of handling the microspheres has become an obstacle that limits its further application
Because the size of the microspheres is too small, it is difficult to distinguish with the naked eye, so in the non-chip detection mode, the microspheres are often damaged artificially, or the microspheres are lost
Since each experiment...

Method used

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  • Photon encoding microsphere-based multielement biomarker assay kit-type chip
  • Photon encoding microsphere-based multielement biomarker assay kit-type chip

Examples

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Embodiment 1

[0029] Example 1: Immobilizing nucleic acid probes on photonic crystal-encoded silica microspheres for analysis of gene expression related to tumor multidrug resistance

[0030] a. Gene sequence screening and nucleic acid probe design: Search for the nucleic acid sequence of the gene to be tested through Gene Bank, and screen the best fragment to be amplified and detected. Hybridization probes for this fragment were designed by eg Primer Express.

[0031] b. Immobilization of nucleic acid probes: the washed microspheres are hydrophilically treated and epoxy silanized, and then the probe molecules modified at one end by amino groups are fixed to the surface of photonic crystal encoded microspheres through covalent bonding;

[0032] c. Encapsulation of the box-type detection chip: fill the template with PDMS, cure it at 70 degrees for 45 minutes and take it out to form the main part of the chip. Then carry out surface hydrophilic treatment with the glass slide at the same time,...

Embodiment 2

[0036] Example 2: Immobilizing protein molecules on photonic crystal-encoded silicon-hydrogel composite microspheres for quantitative analysis of the same protein molecules in serum

[0037] a. Immobilization of protein molecules: the washed microspheres are hydrophilically treated and epoxy silanized, and then the protein molecules are fixed to the surface of the photonic crystal encoded microspheres through the oxidation-reduction reaction of amino groups and epoxy groups;

[0038] b. Encapsulation of the box-type detection chip: fill the template with PDMS, cure at 70°C for 45 minutes and take it out to form the main part of the chip. Then carry out surface hydrophilic treatment with the glass slide at the same time, and then put the treated microspheres into the reaction chamber, and thermally bond the glass slide and the PDMS chip body;

[0039] c. Reaction: push the mixed solution of the serum sample to be tested and a slightly excess fluorescently labeled antibody throu...

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Abstract

The invention discloses a photon encoding microsphere-based multielement biomarker assay kit-type chip. The photon encoding microsphere-based multielement biomarker assay kit-type chip comprises a chip main body at an upper part and a substrate at a lower part. The interior of the chip main body is etched with multiple independent detection units. Each one of the detection units comprises an exhaust vent, a reaction cabin, a channel and a sampling hole. The sampling hole is communicated with the reaction cabin by the channel. The exhaust vent communicated with environment outside the chip main body is arranged in the reaction cabin. Microspheres as detection carriers are enclosed in the reaction cabin and biological molecular probes are fixed on surfaces of the microspheres. According to the invention, a photonic crystal liquid phase chip technology as a base and a self-developed PDMS kit-type chip are combined so that operation difficulty of a photonic crystal liquid phase chip analysis technology is effectively reduced and an operation process is standardized and later-stage automatic detection development is promoted. The detection method adopting the photon encoding microsphere-based multielement biomarker assay kit-type chip is simple and efficient and can replace the existing clinical analysis technology.

Description

technical field [0001] The invention relates to a multi-component biomarker detection cassette chip based on photon-coded microspheres and a preparation method thereof. It can be used to detect biological macromolecules such as proteins and nucleic acids, and can be widely used in clinical testing, inspection and quarantine, environmental monitoring, drug screening, microbial identification, and functional analysis of nucleic acids and proteins. Background technique [0002] Multiplex detection technology has attracted extensive attention due to its high-throughput and low-time-consuming methodological characteristics. Its initial form is a gene chip, which is mainly used in genomics research. Until the end of the last century, the emergence of liquid-phase chip technology made multiple detection technologies emerge in proteomics research and clinical practical applications, and were mostly used in the screening of new markers, the analysis of large samples, and the typing ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 赵祥伟杨子学王晓霞顾忠泽陈宝安
Owner 南京环朋医疗技术有限公司
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