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Cotesia plutellae kurdjumov attractant and preparation method thereof

A technology of velvet wasps and attractants, which is applied in the fields of attracting pests, botanical equipment and methods, and biocides, and can solve the problems of low conidia survival rate, easy inactivation of virus polyhedrons, and difficulties in industrial production, etc. problems, to achieve the effect of inhibiting survival and reproductive ability, enriching raw materials, and weakening survival and reproductive ability

Active Publication Date: 2014-04-30
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with Bt insecticides, Beauveria bassiana preparations have two advantages: one is that many isolates have a wider host range, which can infect a variety of insects; the other is that Beauveria bassiana infects hosts by infecting the cuticle, In addition to caterpillars, beetles and other insects with chewing mouthparts, aphids and leafhoppers can also be infected, but the amount of use is large and the production cost is high; Beauveria bassiana will produce budding spores through fermentation in liquid medium, and its wall is relatively thick. Thin, has the disadvantages of low spore survival rate and short storage period, and needs to be improved (Federici, B. A., Microbial pesticide [J].Pesticide Outlook, 1996, 38(6):22- 27.)
At present, nuclear polyhedrosis virus, cytoplasmic polyhedrosis virus and granular virus are widely studied and widely used. They have the advantages of pathogenicity, specificity and stress resistance, but there are also difficulties in industrial production. , Viral polyhedrons are easily inactivated under ultraviolet light and sunlight and many other problems (Zhou Lifei, Yang Dejun. Research Overview of Insect Virus Insecticides[J]. Plant Doctor, 2007, 20(3) :8-9.)

Method used

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  • Cotesia plutellae kurdjumov attractant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] One, the preparation of the formula that lures Plutella xylostella velvet wasp

[0030] 1.1 Preparation of 100μl / ml single component solution

[0031] Preparation of 100 μl / ml benzaldehyde solution: Add 100 μl of benzaldehyde standard compound to 900 μl of liquid paraffin oil, and mix well.

[0032] Preparation of 100 μl / ml farnesene solution: 100 μl of farnesene standard compound was added to 900 μl of liquid paraffin oil, and mixed evenly.

[0033] Preparation of 100 μl / ml benzyl nitrile solution: 100 μl of benzyl nitrile standard compound was added to 900 μl of liquid paraffin oil, and mixed evenly.

[0034] Preparation of 100 μl / ml cis-3-hexenol solution: 100 μl of cis-3-hexenol standard compound was added to 900 μl of liquid paraffin oil, and mixed evenly.

[0035] Preparation of 100 μl / ml trans-2-hexenal solution: 100 μl of trans-2-hexenal standard compound was added to 900 μl of liquid paraffin oil, and mixed evenly.

[0036] Preparation of 100 μl / ml cineole s...

Embodiment 2

[0045] Take 100 μl of the compound obtained in the above step 1.2 and drop evenly on the filter paper strip and put it into the flavor source bottle on one side, and use the filter paper dripped with 100 μl of liquid paraffin on the other side as a control, and use a "Y" type olfactometer to measure Approximate responses of Plutella xylostella xylostella to a mixture. The Plutella xylostella xylostella wasp with intact antennae and more active behavior was selected for testing. During biotesting, a single Plutella xylostella wasp was placed at the base of the "Y"-shaped tube from the nozzle of the release tube, and then the behavioral response of Plutella xylostella to the odor source within 10 min was observed and recorded. When the wasp climbed over 2 / 3 of a certain arm and lasted for more than 30 s, it was recorded that the parasitic wasp had made a choice for the odor source. If the wasp still did not make a choice after 10 min of introduction, then It was determined that...

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Abstract

The present invention discloses a cotesia plutellae kurdjumov attractant and a preparation method thereof, wherein liquid paraffin is adopted as a solvent, and 100 mul / ml benzaldehyde, farnesene, phenylacetonitrile, cis-3-hexenol, trans-2-hexenal, eucalyptol and nonanal are subjected to the same ratio mixing to obtain the attractant. The attractant is specific component screened from plant volatile substances, and has characteristics of high selectivity and strong targeting property. With the attractant, cotesia plutellae kurdjumov early enters a target prevention and control field, enters a search state, and do not flee so as to enhance host search and attack efficiency, increase parasitism rate, and provide important importance for cotesia plutellae kurdjumov pest controlling effect increase, vegetable field pest management improvement and the like.

Description

technical field [0001] The invention belongs to the technical field of biological control of vegetable pests, and in particular relates to an attractant for Plutella xylostella xylostella and a preparation method thereof. Background technique [0002] diamondback moth ( Plutella xylostella Linnaeus) belongs to Lepidoptera Plutellaidae and is an important pest of cruciferous vegetables worldwide. It has many annual generations, serious generation overlap, high reproduction coefficient, and wide host range. It is distributed in all provinces of my country. It is distributed in the middle and lower reaches of the Yangtze River and The occurrence and damage of diamondback moth are particularly rampant in the south of the country, and have become the main factor affecting the yield and quality of vegetables. According to incomplete statistics, the cost of controlling diamondback moth in the world reaches 4-5 billion U.S. dollars every year (Furlong, M.J., D.J. Wright, L.M. Dosda...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01P19/00A01N27/00A01N31/08A01N31/02A01N37/34
Inventor 杨广张游南尤民生魏辉
Owner FUJIAN AGRI & FORESTRY UNIV
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