Preparation method and application of Maca alkaloid

A technology of maca alkaloids and lye, which is applied in the direction of drug combination, antineoplastic drugs, organic chemistry, etc., can solve the problems of inapplicability to large-scale industrial production, low recovery rate of alkaloids, large amount of solvent consumption, etc. The effect of large size, short process cycle and simple operation

Active Publication Date: 2013-09-04
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned patents can obtain high-purity alkaloids, the monomer components are obtained by repeated chromatography. This method is not only complicated in proces

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of maca alkaloid

[0023] Take 1 kg of maca powder, add 6L of 20% ethanol solution, mix well, ultrasonically extract for 35 minutes, filter, and ultrasonically extract the residue twice according to the above method, combine the filtrates and recover under reduced pressure until there is no alcohol smell, to obtain an extract. Dissolve the above extract with 1% HCl, filter, and adjust the pH value of the filtrate to 9 with sodium hydroxide, then extract with chloroform, concentrate the extract under reduced pressure, and dry to obtain a crude extract of maca alkaloids.

[0024] Mix n-hexane: ethyl acetate: methanol: water in a volume ratio of 5:1:5:1, let stand overnight in a separatory funnel, separate the upper and lower phases, and degas the upper and lower phases ultrasonically for 20 minutes for later use. Take 200mg of maca alkaloid crude extract and dissolve it in 20ml of the lower phase, shake it to dissolve completely, and prepare ...

Embodiment 2

[0026] Embodiment 2: the preparation of maca alkaloid

[0027] Take 5kg of maca powder, add 50L of 60% methanol solution, mix well, extract by microwave for 20min, filter, extract the residue once more according to the above method, combine the filtrate and recover it under reduced pressure until it has no alcohol smell, and obtain the extract. Dissolve the above extract with 2.5% acetic acid, filter, adjust the pH value of the filtrate to 10 with ammonia water, then extract with ethyl acetate, concentrate the extract under reduced pressure, and dry to obtain the crude extract of maca alkaloids.

[0028]Mix n-hexane: ethyl acetate: methanol: water in a volume ratio of 5:5:7:5, let stand overnight in a separatory funnel, separate the upper and lower phases, add 10mM triethylamine to the upper phase, and add 10mM HCl, and ultrasonically degassed for 15min for later use. Take 300mg of maca alkaloid crude extract and dissolve it in 20ml of the lower phase, shake it to dissolve co...

Embodiment 3

[0030] Embodiment 3: the preparation of maca alkaloid

[0031] Take 3kg of maca powder, add 45L of 50% methanol, mix well, extract for 15 minutes, filter, extract the residue twice according to the above method, combine the filtrate and recover it under reduced pressure until it has no alcohol smell, and obtain the extract. Dissolve the above extract with 3% HCl, filter, and adjust the pH value of the filtrate to 11 with sodium carbonate, then extract with ethyl acetate, concentrate the extract under reduced pressure, and dry to obtain a crude extract of maca alkaloids.

[0032] Mix n-hexane: ethyl acetate: ethanol: water in a volume ratio of 6:3:5:7, let stand overnight in a separatory funnel, separate the upper and lower phases, and degas the upper and lower phases ultrasonically for 30 minutes for later use. Take 400mg of maca alkaloid crude extract and dissolve in 20ml equal volume of the upper and lower phases, shake to make it completely dissolved, and prepare for high-s...

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Abstract

The invention discloses a preparation method and an application of Maca alkaloid. The method comprises the steps in sequence as follows: (1), Maca is smashed, a certain volume of an acid alcohol solution is added, supersonic extraction is performed for 1-3 times, the mixture is filtered, and an extracting solution is combined and concentrated, so that extractum is obtained; (2), the extractum is dissolved with acid water and filtered, the pH value is adjusted to be 9-12, the mixture is leached with chloroform or ethyl acetate, and an extraction liquid is subjected to pressure reduction concentration and dried, so that a Maca alkaloid crude extract is obtained; and (3), the Maca alkaloid crude extract is subjected to column chromatography with high-speed countercurrent and alkaline alumina, an eluent comprising a target compound is combined, and a solvent is recycled, so that a Maca alkaloid compound is obtained. According to the method, the technological operation is simple, the preparation amount is large, the sample loss is small, the separation effect is good, the energy consumption is low, the pollution is small the method is suitable for large-scale preparation, and a Maca alkaloid monomer obtained through separation is high in purity, has a better antineoplastic activity, is suitable for preparing antineoplastic drugs and neoplasm prevention health-care food, and has a better application and development prospect.

Description

technical field [0001] The invention belongs to the extraction of effective plant components in the technical field of separation and purification of natural products, and in particular relates to a preparation method and application of maca alkaloids. Background technique [0002] Maca (Lepidium meyenii Walp.) is a herbaceous plant belonging to the genus Brassicaceae. It is native to the Andes Mountains at an altitude of 3500m-4450m in Peru. Growth, known as "Peruvian ginseng" in the world. High nutritional value, reasonable nutritional structure and a variety of active secondary metabolites make maca have a variety of nutritional health functions and pharmacological effects, which can be used to enhance energy, improve fertility, improve sexual function, treat menopausal syndrome, rheumatism, Depression, anemia, etc., as well as anti-cancer and anti-leukemia effects. [0003] Studies have shown that alkaloids are one of the main active ingredients of Maca. Maca alkaloids...

Claims

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Application Information

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IPC IPC(8): C07D233/58A61P35/00
Inventor 赵兵王丽卫张利军
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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