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Complex microbial inoculants of bifidobacterium longum and bifidobacterium animalis and preparation method of complex microbial inoculants

A technology of animal bifidobacteria and longum bifidobacteria, applied in the field of microorganisms, can solve problems such as damage to human health, achieve strong resistance to adverse environments, easy cultivation and preservation, and prevent the occurrence of intestinal cancer and tumors. effect of growth

Inactive Publication Date: 2013-09-04
哈尔滨美华生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be said that without the intestinal probiotic film, human health will be seriously damaged

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The percentages involved in the present invention are all percentages by weight.

[0021] 1. Collect samples from the feces of centenarians in Bama, Guangxi, sterilize the sampling tools and various sampling containers, and collect samples from the feces of many people.

[0022] 2. Serially dilute the collected samples: Mix a certain sample collected evenly under sterile conditions, weigh 10 grams of the sample, dissolve it in 100 mL of sterile water, which contains 100 glass beads, shake vigorously for 10 minutes, Stand still for one minute, draw 1mL supernatant with a 1mL sterile pipette, transfer to a 9mL sterile water test tube, shake thoroughly, then take 1mL sample from this diluted sample, transfer it to a 9mL sterile test tube, and shake thoroughly , so that the dilution is performed in turn until the concentration is 10 -5 .

[0023] 3. Separation method: use Hungate anaerobic rolling tube technology to separate, take out 0.1mL sample from the above diluted s...

Embodiment 2

[0037] A preparation method of a composite microbial agent of Bifidobacterium longum and Bifidobacterium animalis,

[0038] The preparation method of Bifidobacterium longum subspecies: medium formula: 2.0% isomaltose oligosaccharide, 1.0% yeast extract, 1.0% soybean peptone, 1.0% tryptone, 1.0% lactose and 0.05% cysteine, pH6. 5. Sterilize at 121°C for 20 minutes.

[0039] Cool to 37° C., and inoculate Bifidobacterium longum subsp. longum BAMA-B05 / BAu-B1024 strains. The culture temperature was 37°C, and the culture time was 24 hours.

[0040] After the cultivation, centrifuge at 5000rpm, then add the freeze-dried protective agent, mix well, and freeze-dry to obtain the freeze-dried bacteria powder, the number of live bacteria of the freeze-dried bacteria powder is ≥1.0×10 10 cfu / g.

[0041] The preparation method of Bifidobacterium animalis milk subspecies: medium formula: 2.0% isomaltooligosaccharide, 1.0% yeast extract, 1.0% soybean peptone, 1.0% tryptone, 1.0% lactose an...

Embodiment 3

[0046] A preparation method of a compound bacterial agent of Bifidobacterium longum and Bifidobacterium animalis, medium formula: 3.0% isomaltooligosaccharide, 1.5% yeast extract, 1.0% soybean peptone, 1.5% tryptone, 2.0% lactose and Cysteine ​​0.05%, pH 6.5, sterilized at 121°C for 20min.

[0047] Cool to 37°C, insert Bifidobacterium longum subsp.longum BAMA-B05 / BAu-B1024 and Bifidobacterium animalis subsp.lactis BAMA-B06 / BAu-B0111 strains. The culture temperature was 37°C, and the culture time was 24 hours. After the cultivation, centrifuge at 5000rpm, then add the freeze-dried protective agent, mix well, and freeze-dry to obtain the freeze-dried bacteria powder, the number of live bacteria of the freeze-dried bacteria powder is ≥1.0×10 10 cfu / g.

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Abstract

The invention relates to complex microbial inoculants of bifidobacterium longum and bifidobacterium animalis and a preparation method of the complex microbial inoculants. A bifidobacterium longum subsp.longum is named BAMA-B05 / BAu-B1024 and stored with a number of CGMCC (China General Microbiological Culture Collection Center) No.7290. A bifidobacterium animalis subsp.lactis is named BAMA-B06 / Bau-B0111 and stored with the number of CGMCC No.7289. Bama lactic acid bacteria complex microbial inoculants are the compound of the BAMA-B05 / BAu-B1024 and the BAMA-B06 / Bau-B0111 which are at the bacteria number ratio of 1:1. The strain is hard to die, short in growth cycle, fast in reproduction rate and easy to culture and store. The complex microbial inoculants can improve the intestinal microecological balance, enhance the immunity of a host, activate the immune cells, and relieve and treat the constipation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a composite bacterial agent of Bifidobacterium longum and Bifidobacterium animal and a preparation method thereof. Background technique [0002] Bama is a Yao Autonomous County in the Guangxi Zhuang Autonomous Region of my country. It is located in the northwest of Guangxi. There are 12 ethnic groups living here, including Yao, Zhuang, and Han, with a total population of more than 240,000. adjoining. Bama is the region with the highest distribution rate of centenarians, and is known as the "Hometown of Longevity in the World". On November 1, 1991, at the 13th meeting of the International Association of Natural Medicine held in Tokyo, Japan, it was named the fifth hometown of longevity in the world. In November 2003, the International Association of Natural Medicine awarded Bama "the hometown of longevity in the world". Certificate. According to the second to ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/00C12R1/01
Inventor 梁金钟肖文娟
Owner 哈尔滨美华生物技术股份有限公司
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