Composite bacillus cottonseed meal fermenting agent and preparation method thereof
A compound Bacillus and cottonseed fermentation technology, applied in the field of microbial fermentation, can solve the problems of low viable count of fermentative inoculants, difficult to stabilize fermentation effect, short storage time, etc., so as to improve animal immunity and eliminate anti-nutrients. Factors, the effect of simple production process
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Embodiment 1
[0023] 1. Prepare various liquid strains:
[0024] Liquid culture medium (4 parts): add glucose 30g, bean cake powder 15g, (NH 4 ) 2 SO 4 4g, KH 2 PO 4 4g, MgSO 4 ·7H 2 O 1.5g, NaCl 5g, and CaCO 3 2g, initial pH7.2;
[0025] Take 1 strain of Bacillus subtilis and inoculate a ring of slanted strain cells into a liquid strain culture medium, and culture it on a shaking table at 37°C and 200r / min for 60h, so that the spore formation rate of the cells in the seed liquid can reach 94.7% , 80°C constant temperature water bath for 12 minutes;
[0026] Take 1 strain of Bacillus lateralis and inoculate a ring of slanted strain cells into the liquid strain culture medium, and culture it on a shaking table at 37°C and 200r / min for 60 hours, so that the spore formation rate of the cells in the seed liquid can reach 92.6% , 80°C constant temperature water bath for 12 minutes;
[0027] Take 1 strain of Bacillus natto and inoculate a ring of slanted strain cells into the liquid ...
Embodiment 2
[0040] 1. Prepare various liquid strains:
[0041] Liquid culture medium (4 parts): 35g of glucose, 12g of bean cake powder, (NH 4 ) 2 SO 4 6g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 2.5g, NaCl3g, and CaCO 3 4g, initial pH7.5;
[0042] Take 1 strain of Bacillus subtilis and inoculate a ring of slanted strain cells into a liquid strain culture medium, and culture it on a shaking table at 40°C and 180r / min for 48 hours, so that the spore formation rate of the cells in the seed liquid can reach 92.7% , 80°C constant temperature water bath for 10 minutes;
[0043] Take 1 strain of Bacillus lateralis and inoculate a ring of slant strain cells into the liquid strain culture medium, and culture it on a shaking table at 40°C and 180r / min for 48 hours, so that the spore formation rate of the cells in the seed liquid can reach 91.3%. , 80°C constant temperature water bath for 10 minutes;
[0044] Take 1 strain of Bacillus natto and inoculate a ring of slanted strain cells into the li...
Embodiment 3
[0057] 1. Prepare various liquid strains:
[0058] Liquid culture medium (4 parts): add glucose 40g, bean cake powder 10g, (NH 4 ) 2 SO 4 2g, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 1g, NaCl4g, and CaCO 3 3g, initial pH7.4;
[0059] Take 1 strain of Bacillus subtilis, Bacillus lateralis, Bacillus natto, and Bacillus coagulans to inoculate a ring of slant strain cells respectively in the liquid culture medium, shake at 39°C and 160r / min Bed shaking culture for 52 hours, so that the sporulation rate of the cells in the seed liquid reached 95%, and treated in a constant temperature water bath at 80°C for 15 minutes, and each liquid strain was prepared respectively;
[0060] (2) Fermentation:
[0061]The above-mentioned Bacillus coagulans liquid strains were inoculated in the Bacillus coagulans fermentation medium, the culture temperature was 37°C, the inoculum size was 4%, the stirring speed was 170r / min, and the fermentation culture time was 50h.
[0062] Bacillus coagulans fe...
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