Micro-fluidic serum extracting chip

A microfluidic and serum technology, applied in the direction of laboratory equipment, test sample preparation, chemical instruments and methods, etc., can solve the problems of blood sample loss, complex mechanical structure, interference of spectral analysis, etc., and achieve simple operation, separation high efficiency effect

Inactive Publication Date: 2013-09-11
苏州扬清芯片科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, blood testing is the most widely used sample testing in biomedicine, clinical diagnosis, health and quarantine diagnosis, but the presence of red blood cells has a great interference on the spectral analysis in blood testing, so how to quickly and easily separate serum from whole blood It has always been a hot and difficult issue in blood testing research
[0003] At present, most hospitals and laboratories still use large centrifuges to separate serum and blood cells. This method requires blood samples in milliliters to be drawn from patients, but the samples actually use

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  • Micro-fluidic serum extracting chip

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Example Embodiment

[0015] Example 1 A polymethyl methacrylate serum extraction chip

[0016] Such as figure 1 As shown, the serum extraction chip provided by the present invention is composed of a sample injection pool A, a microchannel B, a serum separation chamber C, and a sample collection pool D. The serum separation chamber is filled with two glass beads (particle size 20μm and 100μm respectively). The chip material is polymethyl methacrylate, and each microstructure is precisely processed by a CNC numerical control machine. The width and depth of the microchannel are 80μm, and the chip is finally sealed by hot pressing.

[0017] Serum separation steps are as follows: add whole blood from the sample pool, the blood sample flows through the serum separation chamber under the action of capillary force or a certain negative pressure, blood cells are trapped at the front end, and the serum flows out from the pores between the beads, and finally enters Sample collection tank. Using the serum extra...

Example Embodiment

[0018] Example 2 A polydimethylsiloxane serum extraction chip

[0019] Such as figure 1 As shown, the serum extraction chip provided by the present invention is composed of a sample injection pool A, a microchannel B, a serum separation chamber C, and a sample collection pool D. The serum separation chamber is filled with two glass beads (particle size 15μm and 120μm respectively). The chip material is polydimethylsiloxane, and the microstructure is processed by a photolithographic mold-based casting polymerization method. The width and concentration of the microchannel are 100μm, and finally, irreversible sealing is adopted.

[0020] Serum separation steps are as follows: add whole blood from the sample pool, the blood sample flows through the serum separation chamber under the action of capillary force or a certain negative pressure, blood cells are trapped at the front end, and the serum flows out from the pores between the beads, and finally enters Sample collection tank. Us...

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Abstract

The invention aims to provide a micro-fluidic serum extracting chip. The chip is formed by a blood sample introduction pool, a micro channel, a serum separation chamber and a sample collecting pool, wherein the interior of the serum separation chamber is filled with two kinds of micro balls with different hole diameters. The operating principle of the chip is as follows: the whole blood is added from a sample introduction port, the blood sample flows through the serum separation chamber along the micro channel under the action of surface tension or certain negative pressure, hemocytes are intercepted by a micro ball structure, and the serum flows out from ventages among the micro balls, and finally enters the sample collecting pool. The chip provided by the invention can be used for rapidly extracting the serum on line, the operation is simple and convenient, the separation efficiency is high, and the further integration with other operation units is easy to realize.

Description

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Claims

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Application Information

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Owner 苏州扬清芯片科技有限公司
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