Micro-fluidic serum extracting chip
A microfluidic and serum technology, applied in the direction of laboratory equipment, test sample preparation, chemical instruments and methods, etc., can solve the problems of blood sample loss, complex mechanical structure, interference of spectral analysis, etc., and achieve simple operation, separation high efficiency effect
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[0015] Example 1 A polymethyl methacrylate serum extraction chip
[0016] Such as figure 1 As shown, the serum extraction chip provided by the present invention is composed of a sample injection pool A, a microchannel B, a serum separation chamber C, and a sample collection pool D. The serum separation chamber is filled with two glass beads (particle size 20μm and 100μm respectively). The chip material is polymethyl methacrylate, and each microstructure is precisely processed by a CNC numerical control machine. The width and depth of the microchannel are 80μm, and the chip is finally sealed by hot pressing.
[0017] Serum separation steps are as follows: add whole blood from the sample pool, the blood sample flows through the serum separation chamber under the action of capillary force or a certain negative pressure, blood cells are trapped at the front end, and the serum flows out from the pores between the beads, and finally enters Sample collection tank. Using the serum extra...
Example Embodiment
[0018] Example 2 A polydimethylsiloxane serum extraction chip
[0019] Such as figure 1 As shown, the serum extraction chip provided by the present invention is composed of a sample injection pool A, a microchannel B, a serum separation chamber C, and a sample collection pool D. The serum separation chamber is filled with two glass beads (particle size 15μm and 120μm respectively). The chip material is polydimethylsiloxane, and the microstructure is processed by a photolithographic mold-based casting polymerization method. The width and concentration of the microchannel are 100μm, and finally, irreversible sealing is adopted.
[0020] Serum separation steps are as follows: add whole blood from the sample pool, the blood sample flows through the serum separation chamber under the action of capillary force or a certain negative pressure, blood cells are trapped at the front end, and the serum flows out from the pores between the beads, and finally enters Sample collection tank. Us...
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