The method of separating rock algae derived and konbard polysaccharides from the live harvest of seaweed
A technology for fucoidan and laminarin, applied in the field of separating fucoidan and laminarin from live harvested seaweed, can solve the problems of not utilizing exudation, complex chemical and mechanical extraction steps, etc.
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Embodiment 1
[0039] Freshly harvested live northern kelp was collected and cut into approximately 10cm pieces. No water was added to the harvested or cut seaweed, which was maintained at ambient temperature. The freshly harvested live seaweed (containing only the amount of seawater mixed with the seaweed at the time of harvest) was kept exuding at a temperature below 30°C for about 3 days, after which about 60 liters of exudate per ton of seaweed was collected.
[0040] Then 950 liters of exudate were set aside, of which 1 liter was used for alcohol precipitation to determine the yield of original fucoidan in the exudate. The exudate was found to have a viscosity of 11.7 cps (using a Brookfield Viscometer (Brookfield Viscometer) with LV interface, spindle number 61, 60 rpm, room temperature), a pH of 5.75, a TBU (Turbidity Unit) of 60 NTU, and 4.4% dry Substance (determined after overnight drying at 105°C). Add the exudate to isopropanol (1 part exudate to 2 parts isopropanol), stir at r...
Embodiment 2
[0043] Freshly harvested live northern kelp is cut into pieces about 1-20cm in size on board. The exudate collected on the bottom of the boat is pumped into a container. No water is added to the harvested or cut seaweed, and the seaweed is maintained at a temperature below 30°C. Freshly harvested live seaweed (containing only the amount of seawater that the seaweed had incorporated at the time of harvest) was exuded for about 7 days at a temperature below 30°C, after which approximately 1,650 liters of exudate were obtained. To this exudate was added 0.3% formalin as a preservative. Alcohol precipitation (using isopropanol, 2 parts alcohol to 1 part exudate) was performed on a portion of the exudate (100 ml) to obtain a fucoidan content of 2.6 g per liter of exudate. The dry matter of the exudate was 5.7% after overnight drying at 105°C.
[0044] After determining the fucoidan yield in the exudate by alcohol precipitation as described above, the remaining exudate (approxima...
Embodiment 3
[0046] Seaweed freshly harvested live northern kelp cut into approximately 10cm pieces. No water was added to the seaweed, which was maintained at ambient temperature. Freshly harvested live seaweed was allowed to exudate for approximately 3 days at room temperature, after which approximately 9,000 liters of exudate were collected. The 9,000 liters of exudate were filtered through a plate and frame filter press to remove large solid debris from the seaweed, and the volume was reduced to 4,000 liters by heat and vacuum.
[0047] 10 ml of the reduced volume filtrate was then analyzed, which had a pH of 6, contained 3-4% dry matter, and had Brix units of 4-5. 200 ml of the reduced volume filtrate was subjected to alcohol precipitation (using isopropanol) as described in Example 1 to remove fucoidan from the filtrate. After alcohol precipitation and removal of fucoidan, the filtrate was left overnight. Laminarin was precipitated from the filtrate and recovered by filtration. T...
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